FlyBase Allele Report: Dmel\kcc[KK107965]

General Information

Symbol

Dmel\kcc^KK107965

Species

D. melanogaster

Name

FlyBase ID

FBal0235281

Feature type

allele

Associated gene

Dmel\kcc

Associated Insertion(s)

Carried in Construct

P{KK107965}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK107965}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK107965

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of epilepsy

CEA

(Rusan et al., 2014)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor rhythm | adult stage, with Hsap\RELA^AD.R12G04, Scer\GAL4^DBD.R18D09

(Eick et al., 2022)

abnormal locomotor rhythm | adult stage, with Scer\GAL4^Clk.-856

(Eick et al., 2022)

abnormal locomotor rhythm | adult stage, with Scer\GAL4^Dvir\Pdf.1.9

(Eick et al., 2022)

abnormal locomotor rhythm | adult stage, with Scer\GAL4^GMR18H11

(Eick et al., 2022)

abnormal locomotor rhythm | adult stage, with Scer\GAL4^NP6258

(Eick et al., 2022)

abnormal locomotor rhythm | adult stage, with Scer\GAL4^tim.PE

(Eick et al., 2022)

abnormal neuroanatomy, with Scer\GAL4^elav-C155

(Rusan et al., 2014)

abnormal neuroanatomy, with Scer\GAL4^shot-OK307

(Rusan et al., 2014)

abnormal neurophysiology, with Scer\GAL4^shot-OK307

(Rusan et al., 2014)

bang sensitive, with Scer\GAL4^repo.PU

(Rusan et al., 2014)

bang sensitive, with Scer\GAL4^shot-OK307

(Rusan et al., 2014)

decreased size | adult stage, with Scer\GAL4^Bx-MS1096

(Yarikipati et al., 2023)

lethal, with Scer\GAL4^Act5C.PI

(Rusan et al., 2014)

lethal, with Scer\GAL4^elav-C155

(Rusan et al., 2014)

paralytic, with Scer\GAL4^repo.PU

(Rusan et al., 2014)

paralytic, with Scer\GAL4^shot-OK307

(Rusan et al., 2014)

Phenotype Manifest In

abdominal nerve | third instar larval stage, with Scer\GAL4^elav-C155

(Rusan et al., 2014)

adult brain, with Scer\GAL4^shot-OK307

(Rusan et al., 2014)

adult pacemaker neuron, with Scer\GAL4^shot-OK307

(Rusan et al., 2014)

dendrite, with Scer\GAL4^shot-OK307

(Rusan et al., 2014)

giant fiber neuron, with Scer\GAL4^shot-OK307

(Rusan et al., 2014)

wing blade, with Scer\GAL4^Bx-MS1096

(Yarikipati et al., 2023)

Detailed Description

Statement

Reference

Expression of kcc^KK107965 under the control of any of the following drivers is largely ineffective in inducing bang-sensitive paralytic behavior: Scer\GAL4^Aph-4-c507 (ellipsoid body), Scer\GAL4^RapGAP1-OK6 (motor neuron) Scer\GAL4^Tab2-201Y, Scer\GAL4^Mef2.247, Scer\GAL4^ey-OK107 or Scer\GAL4^c747 (mushroom body).

Expression of kcc^KK107965 in glial cells under the control of Scer\GAL4^repo.PU results in substantial bang-sensitive paralytic behavior.

Expression of kcc^KK107965 in a subset of neuronal cells under the control of Scer\GAL4^OK307 results in substantial bang-sensitive paralytic behavior. The threshold to evoked seizure-like discharges in these flies is 1/4 of that of wild type. Brain volume is increased compared to controls. Giant fiber and lateral pace-making neurons are enlarged compared to controls. The enlarged giant fiber neurons expressing exhibit diffuse and elongated dendrites that lack distinctive spines.

Expression of kcc^KK107965 under the control of Scer\GAL4^elav-C155 causes nerve swelling. Third instar larval abdominal nerves display an increase in the average never cross-sectional area compared to controls.

(Rusan et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

kcc^KK107965/Scer\GAL4^Bx-MS1096 is a non-enhancer of decreased size | adult stage phenotype of Scer\GAL4^Bx-MS1096, Wnk^{D420A.UAS.cWa}

(Yarikipati et al., 2023)

Suppressor of

Statement

Reference

kcc^KK107965/Scer\GAL4^P2.4.Pdf is a suppressor of abnormal locomotor rhythm | adult stage phenotype of Ncc69^r2

(Schellinger et al., 2022)

NOT Suppressor of

Statement

Reference

kcc^KK107965/Scer\GAL4^Bx-MS1096 is a non-suppressor of decreased size | adult stage phenotype of Scer\GAL4^Bx-MS1096, Wnk^{D420A.UAS.cWa}

(Yarikipati et al., 2023)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

kcc^KK107965/Scer\GAL4^Bx-MS1096 is a non-enhancer of wing blade phenotype of Scer\GAL4^Bx-MS1096, Wnk^{D420A.UAS.cWa}

(Yarikipati et al., 2023)

NOT Suppressor of

Statement

Reference

kcc^KK107965/Scer\GAL4^Bx-MS1096 is a non-suppressor of wing blade phenotype of Scer\GAL4^Bx-MS1096, Wnk^{D420A.UAS.cWa}

(Yarikipati et al., 2023)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v101742

P{KK107965}VIE-260B

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

CG5594^KK107965

kcc^IR101742

(Yarikipati et al., 2023)

kcc^KK107965

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

Open Close