FlyBase Gene Report: Dmel\Ask1

General Information

Symbol

Dmel\Ask1

Species

D. melanogaster

Name

Apoptotic signal-regulating kinase 1

Annotation Symbol

CG4720

Feature Type

protein_coding_gene

FlyBase ID

FBgn0014006

Gene Model Status

Current

Stock Availability

12 publicly available

Enzyme Name (EC)

mitogen-activated protein kinase kinase kinase (2.7.11.25)

Gene Summary

Apoptotic signal-regulating kinase 1 (Ask1) encodes a serine/threonine kinase that belongs to the MAPK kinase kinase family. In response to various stresses, it phosphorylates MAPK kinases in JNK and p38 MAPK pathways, inducing cellular stress responses including cell death and immune responses. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

Pk92B, DASK1

Key Links

Genomic Location

Cytogenetic map

92B4-92B4

Sequence location

3R:19,879,719..19,886,030 [+]

Recombination map

3-67

RefSeq locus

NT_033777 REGION:19879719..19886030

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (9 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables MAP kinase kinase kinase activity

inferred from direct assay

(Kuranaga et al., 2002)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables ATP binding

inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR017441

(InterPro Project Members, 2004-)

enables MAP kinase kinase kinase activity

inferred from sequence or structural similarity with UniProtKB:Q99683

(Gene Ontology Curators, 2002-)

enables protein kinase activity

inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR008271

(InterPro Project Members, 2004-)

inferred from biological aspect of ancestor with PANTHER:PTN000172316

(GO Reference Genome Project, 2011-)

Biological Process (5 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

involved_in JNK cascade

inferred from direct assay

(Sekine et al., 2011, Kuranaga et al., 2002)

inferred from mutant phenotype

(Sekine et al., 2011, Ryabinina et al., 2006)

involved_in positive regulation of JNK cascade

inferred from direct assay

(Sekine et al., 2011)

involved_in regulation of adult chitin-containing cuticle pigmentation

inferred from mutant phenotype

(Sekine et al., 2011)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

involved_in JNK cascade

inferred from sequence or structural similarity with UniProtKB:Q99683

(Gene Ontology Curators, 2002-)

inferred from biological aspect of ancestor with PANTHER:PTN000172316

(GO Reference Genome Project, 2011-)

involved_in MAPK cascade

inferred from electronic annotation with InterPro:IPR043969

(InterPro Project Members, 2004-)

inferred from sequence or structural similarity with UniProtKB:Q99683

(Gene Ontology Curators, 2002-)

involved_in p38MAPK cascade

inferred from biological aspect of ancestor with PANTHER:PTN000172316

(GO Reference Genome Project, 2011-)

Cellular Component (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

part_of protein kinase complex

inferred from sequence or structural similarity with UniProtKB:Q99683

(Gene Ontology Curators, 2002-)

Gene Group (FlyBase)

STE11 MAP KINASE KINASE KINASES

Protein Family (UniProt)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

MAP kinase kinase kinase activity

(1) L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+) (2.7.11.25)

(2) L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+) (2.7.11.25)

Summaries

Gene Snapshot

Gene Group (FlyBase)

STE11 MAP KINASE KINASE KINASES -: STE11 MAP kinase kinase kinases (MAPKKKs) belong to the STE11 subfamily and are serine/threonine kinases that phosphorylate MAP kinase kinases in MAPK signalling cascades. MAPKKKs are activated by MAP kinase kinase kinase kinases (MAP4Ks). (Adapted from FBrf0225276.)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Ask1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VDS9)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.54

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Ask1-RB

FBtr0083819

NM_206518

5168

1196

Ask1-RC

FBtr0300256

NM_057741

4596

1363

Additional Transcript Data and Comments

Reported size (kB)

5.108 (longest cDNA)

(Wassarman et al., 1996)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Ask1-PB

FBpp0089256

136.5

1196

6.97

FBpp0289484

155.5

1363

6.81

Q9VDS9

NP_477089

AAF55711

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

650 (aa)

(Wassarman et al., 1996)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Ask1 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Pk92B_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.44

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

Reporter: Mi{Trojan-GAL4.1}Ask1^{MI02915-TG4.2}

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult brain | restricted

(Lee et al., 2018)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Ask1 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Ask1

View exonic expression by tissue for Dmel\Ask1

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

42366/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0014006

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0014006

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0014006

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG4720

Flygut - An atlas of the Drosophila adult midgut

FBgn0014006

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 6 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 15 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Ask1

Transgenic constructs containing regulatory region of Ask1

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(3R)ED6027

(Miller et al., 2020)

Inferred from location ( 5 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal

Ask1^MI02915

lethal (with Df(3R)ED6027)

Ask1^MI02915

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

Ask1^GD11408

viable

Ask1^{MI02915-GFSTF.2}

viable, with Scer\GAL4^pnr-MD237

Ask1^GD11408

viable, with Scer\GAL4^tin.CΔ4

Ask1^GD11408

Sterility

Allele

female semi-sterile, with Scer\GAL4^{VP16.nanos.UTR}, Scer\GAL4^VP16.otu, Scer\GAL4^nanos.PG

Other Phenotypes

Allele

abnormal body color, with Scer\GAL4^pnr-MD237

Ask1^{ΔN.UAS.Tag:FLAG}

abnormal body color | heat sensitive, with Scer\GAL4^hs.PU

Ask1^{ΔN.UAS.Tag:FLAG}

abnormal neuroanatomy | larval stage, with Scer\GAL4^tey-5053A

Ask1^GL00238

abnormal stress response | adult stage

Ask1^MI02915

Phenotype manifest in

Allele

adult cuticle, with Scer\GAL4^pnr-MD237

Ask1^{ΔN.UAS.Tag:FLAG}

adult thorax, with Scer\GAL4^pnr-MD237

Ask1^{ΔN.UAS.Tag:FLAG}

adult thorax | heat sensitive, with Scer\GAL4^hs.PU

Ask1^{ΔN.UAS.Tag:FLAG}

axon | adult stage

Ask1^MI02915

distal axon | larval stage, with Scer\GAL4^tey-5053A

Ask1^GL00238

embryo | maternal effect, with Scer\GAL4^{VP16.nanos.UTR}, Scer\GAL4^VP16.otu, Scer\GAL4^nanos.PG

Ask1^GL00238

embryonic/larval motor neuron | larval stage, with Scer\GAL4^tey-5053A

Ask1^GL00238

wing | heat sensitive, with Scer\GAL4^hs.PU

Ask1^{ΔN.UAS.Tag:FLAG}

wing nerve

Ask1^MI02915

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (59)

Hsap\MAP3K15

14 of 14

Yes

Hsap\MAP3K5

13 of 14

Yes

Hsap\MAP3K6

9 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Hsap\LOC407835

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (28)

Rnor\Map3k5

13 of 14

Yes

Rnor\Map3k6

11 of 14

Yes

Rnor\Map3k15

7 of 14

Yes

Rnor\Map3k1

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (33)

Mmus\Map3k15

14 of 14

Yes

Mmus\Map3k5

12 of 14

Yes

Mmus\Map3k6

9 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Gm10668

1 of 14

Mmus\Map3k1

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Map3k19

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (31)

Xtro\map3k5

6 of 13

Yes

Xtro\map3k5

6 of 13

Yes

Xtro\map3k15

5 of 13

Yes

5 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\LOC101734050

1 of 13

Yes

Xtro\map2k1

1 of 13

Xtro\map3k1

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (35)

Drer\map3k5

13 of 14

Yes

Drer\map3k15

13 of 14

Yes

Drer\si:ch211-1i11.3

11 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Drer\si:ch211-45c16.2

1 of 14

Drer\si:dkey-151g10.3

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (23)

Cele\nsy-1

13 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Cele\flr-4

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (23)

Agam\AgaP_AGAP000747

11 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (100)

Atha\MAP3KA

3 of 13

Yes

Atha\MAPKKK5

3 of 13

Yes

Atha\MAPKKK6

3 of 13

Yes

Atha\MAPKKK7

3 of 13

Yes

Atha\MEKK1

3 of 13

Yes

Atha\NP1

3 of 13

Yes

Atha\NP2

3 of 13

Yes

Atha\NP3

3 of 13

Yes

Atha\YDA

3 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Atha\AT2G18530

1 of 13

Yes

Atha\AT2G24360

1 of 13

Atha\AT2G34290

1 of 13

Yes

Atha\AT2G35050

1 of 13

Atha\AT2G40500

1 of 13

Yes

Atha\AT2G40560

1 of 13

Yes

Atha\AT2G40580

1 of 13

Yes

Atha\AT2G41910

1 of 13

Yes

Atha\AT2G41920

1 of 13

Yes

Atha\AT2G41930

1 of 13

Yes

Atha\AT2G42550

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Atha\AT3G45790

1 of 13

Yes

Atha\AT3G46140

1 of 13

Yes

Atha\AT3G46160

1 of 13

Yes

Atha\AT3G46930

1 of 13

Atha\AT3G53930

1 of 13

Atha\AT4G04632

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Atha\AT5G18700

1 of 13

Atha\AT5G27510

1 of 13

Yes

Atha\AT5G27790

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Atha\MAPKKK14

1 of 13

Yes

Atha\MAPKKK15

1 of 13

Yes

Atha\MAPKKK16

1 of 13

Yes

Atha\MAPKKK17

1 of 13

Yes

Atha\MAPKKK18

1 of 13

Yes

Atha\MAPKKK19

1 of 13

Yes

Atha\MAPKKK20

1 of 13

Yes

Atha\MAPKKK21

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (13)

Scer\STE11

4 of 13

Yes

Scer\BCK1

3 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Schizosaccharomyces pombe (Fission yeast) (6)

Spom\byr2

3 of 12

Yes

Spom\mkh1

3 of 12

Yes

Spom\ark1

1 of 12

Spom\byr1

1 of 12

Spom\cdc7

1 of 12

Yes

Spom\wis4

1 of 12

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Ask1. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (26)

Mekk1

3 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

grp

1 of 13

hpo

1 of 13

Madm

1 of 13

mbt

1 of 13

Mos

1 of 13

Nek2

1 of 13

niki

1 of 13

Pak3

1 of 13

Pak

1 of 13

Pka-C1

1 of 13

PKD

1 of 13

put

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

wit

1 of 13

wnd

1 of 13

Wnk

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

Ask1^HMS00464

ameliorates amyotrophic lateral sclerosis type 10

modeled by Hsap\TARDBP^{M337V.UAS.Tag:MYC}

(Kankel et al., 2020)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

MAP3K15; mitogen-activated protein kinase kinase kinase 15

14 of 14

300820

MAP3K5; mitogen-activated protein kinase kinase kinase 5

13 of 14

602448

MAP3K6; mitogen-activated protein kinase kinase kinase 6

9 of 14

604468

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Ask1 - AIF

experimental knowledge based

(Guruharsha et al., 2011)

Ask1 - Bcs1

experimental knowledge based

(Guruharsha et al., 2011)

Ask1 - CG10494

experimental knowledge based

(Guruharsha et al., 2011)

Ask1 - CG1513

experimental knowledge based

(Guruharsha et al., 2011)

Ask1 - CG33523

experimental knowledge based

(Guruharsha et al., 2011)

Ask1 - CG9205

experimental knowledge based

(Guruharsha et al., 2011)

Ask1 - GAPcenA

experimental knowledge based

(Guruharsha et al., 2011)

Ask1 - Nup358

experimental knowledge based

(Guruharsha et al., 2011)

Ask1 - RanGAP

experimental knowledge based

(Guruharsha et al., 2011)

Ask1 - Sk2

experimental knowledge based

(Guruharsha et al., 2011)

Ask1 - az2

experimental knowledge based

(Guruharsha et al., 2011)

Ask1 - cert

experimental knowledge based

(Guruharsha et al., 2011)

Ask1 - cliff

experimental knowledge based

(Guruharsha et al., 2011)

Ask1 - l(2)k01209

experimental knowledge based

(Guruharsha et al., 2011)

Ask1 - rdgB

experimental knowledge based

(Guruharsha et al., 2011)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Ask1

suppressible

Ddc

(Sekine et al., 2011)

Ask1

suppressible

(Sekine et al., 2011)

Ask1

suppressible

hep

(Sekine et al., 2011)

Ask1

suppressible

Hr38

(Sekine et al., 2011)

Ask1

suppressible

lic

(Sekine et al., 2011)

Ask1

suppressible

Mkk4

(Sekine et al., 2011)

Ask1

suppressible

p38a

(Sekine et al., 2011)

Ask1

suppressible

p38b

(Sekine et al., 2011)

Ask1

suppressible

p38c

(Sekine et al., 2011)

Ask1

suppressible

ple

(Sekine et al., 2011)

Ask1

suppressible

(Sekine et al., 2011)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Cha et al., 2005)

enhanceable

(Cha et al., 2005)

suppressible

(Kuranaga et al., 2002)

slim

enhanceable

Ask1

(Sekine et al., 2012)

slim

suppressible

Ask1

(Sekine et al., 2012)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

67355

DroID - A comprehensive database of gene and protein interactions.

FBgn0014006

MIST (genetic) - An integrated Molecular Interaction Database

42366

MIST (protein-protein) - An integrated Molecular Interaction Database

42366

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Oxidative Stress Induced Senescence

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q24458

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-67

Cytogenetic map

92B4-92B4

Sequence location

3R:19,879,719..19,886,030 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

92B4-92B4

Limits computationally determined from genome sequence between P{PZ}l(3)10585¹⁰⁵⁸⁵ and P{EP}Synd^EP409

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

92B-92B

(Kuranaga, 2000.2.9)

92B8-92B10

(Wassarman, 1995.6.15)

92B8-92B10

(determined by in situ hybridisation)

(Wassarman et al., 1996)

Experimentally Determined Recombination Data

Location

3-67

(FlyBase, 2016)

3-69.5

(Comeron et al., 2012)

(Wassarman, 1995.6.15)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (12)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

99489

w^*; P{UAS-Ask1.K618M}2/CyO

26048

w¹¹¹⁸; Mi{ET1}Ask1^MB06487

80892

y¹, v¹; P{TKO.GS04796}attP40/CyO

59799

y¹ w^*; Mi{PT-GFSTF.2}Ask1^{MI02915-GFSTF.2}

35331

y¹ sc^* v¹ sev²¹; P{TRiP.GL00238}attP2

32464

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00464}attP2

36163

y¹ w^*; Mi{MIC}Ask1^MI02915/TM3, Sb¹ Ser¹

NIG-Fly

GL00238

y¹ sc^* v¹ sev²¹; P{TRiP.GL00238}attP2

HMS00464

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00464}attP2

VDRC

v110228

P{KK102892}VIE-260B

v34891

w¹¹¹⁸; P{GD11408}v34891

v342635

P{hsFLP}1, y¹ w¹¹¹⁸; P{HD_CFD00806}attP40/CyO-GFP

Genomic Clones (10)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (45)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

RE60627

Other clones

FI02066

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0014006

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0014006

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, an increase in the proportion of G1 phase cells is seen.

(Bettencourt-Dias et al., 2004)

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in S2R⁺ cells: cells become retracted (unspread but flat). Kc167 cells are unaffected.

(Kiger et al., 2003)

Isolated from an eye-antennal disc cDNA library, using PCR with degenerate primers complementary to sub-domains VI and IX of the catalytic domain of Ser/Thr protein kinases.

(Wassarman et al., 1996)

A Pk92B cDNA has been cloned and sequenced.

(Wassarman et al., 1996)

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: Pk92B CG4720

(Gong, 1999.11)

Source for identity of: Ask1 Pk92B

(FlyBase, 2009-)

Nomenclature comments

Renamed from 'Pk92B' to 'Ask1' to give the gene a more informative symbol/name that reflects usage in the literature.

(FlyBase, 2009-)

Etymology

Synonyms and Secondary IDs (17)

Reported As

Symbol Synonym

ASK1

(Guo et al., 2026, Adell et al., 2025, Gan et al., 2021, Biteau et al., 2011, Milton et al., 2011, Sekine et al., 2011, Hay et al., 2004)

Ask

(Xu et al., 2022)

CG4720

(Ni et al., 2011.7.19, Ni et al., 2010.12.1, Ardehali et al., 2009, Keleman et al., 2009.8.5, Morrison et al., 2000)

D-ASK

(Ryabinina et al., 2006)

DASK

(Kuranaga, 2000.2.9)

DASK1

(Mosallanejad et al., 2014, Ríos-Barrera and Riesgo-Escovar, 2013, Sekine et al., 2012, Sekine et al., 2011, Takeda et al., 2009, Takeda et al., 2009, Cha et al., 2005, Kuranaga et al., 2003, Kuranaga et al., 2002)

DPk92B/ASK1

(Chen et al., 2002)

MEKK5/ASK1

(Morrison et al., 2000)

Pk92B

(Stephano et al., 2018, Song et al., 2017, Bartok et al., 2015, Gene Disruption Project members, 2015-, Zirin et al., 2015, Sopko et al., 2014, Wong et al., 2014, Kong et al., 2010, Lesch et al., 2010, Moshkin et al., 2009, Owusu-Ansah et al., 2008)

ask1

(Hsu et al., 2021, Joy et al., 2021)

dASK

(Domingues and Ryoo, 2008)

dASK1

(Mishra et al., 2021, Zhuang et al., 2006)

dAsk1

(Neisch et al., 2010)

pk92B

(Zhang et al., 2018, Ardehali et al., 2009)

Name Synonyms

Apoptotic signal-regulating kinase 1

(Neisch et al., 2010, Kuranaga et al., 2003)

Protein kinase at 92B

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 53 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

42366

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VDS9

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VDS9

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

42366/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0014006

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0014006

FlyMine - An integrated database for Drosophila genomics

FBgn0014006

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG4720

MARRVEL_MODEL - MARRVEL (model organism gene)

42366

Linkouts

BioGRID - A database of protein and genetic interactions.

67355

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0014006

DroID - A comprehensive database of gene and protein interactions.

FBgn0014006

DRSC - Results frm RNAi screens

FBgn0014006

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Pk92B_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0014006

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0014006

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG4720

Flygut - An atlas of the Drosophila adult midgut

FBgn0014006

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0014006

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC000430

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

MIST (genetic) - An integrated Molecular Interaction Database

42366

MIST (protein-protein) - An integrated Molecular Interaction Database

42366

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Oxidative Stress Induced Senescence

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q24458

References (129)

The following external sites may use different assemblies or annotations than FlyBase.

-0.44

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