FlyBase Gene Report: Dmel\Dsor1

General Information

Symbol

Dmel\Dsor1

Species

D. melanogaster

Name

Downstream of raf1

Annotation Symbol

CG15793

Feature Type

protein_coding_gene

FlyBase ID

FBgn0010269

Gene Model Status

Current

Stock Availability

23 publicly available

Enzyme Name (EC)

mitogen-activated protein kinase kinase (2.7.12.2)

Gene Summary

Downstream of raf1 (Dsor1) encodes a serine/threonine kinase that phosphorylates MAP kinase. It is activated by the product of Raf and acts downstream of receptor tyrosine kinases such as those encoded by tor, Egfr, and sev. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Pathway
Gene Group
UniProtKB
Interactive Fly

Also Known As

MEK, Dsor, MAPKK, D-mek, DRODSOR1

Key Links

Genomic Location

Cytogenetic map

8D2-8D3

Sequence location

X:9,247,342..9,250,037 [+]

Recombination map

1-27

RefSeq locus

NC_004354 REGION:9247342..9250037

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (26 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

enables kinase binding

inferred from physical interaction with UniProtKB:P11346,UniProtKB:Q24171

(Lavoie et al., 2018)

enables MAP kinase kinase activity

inferred from direct assay

(Oellers and Hafen, 1996)

inferred from genetic interaction with SGD:S000004354

(Lim et al., 1997)

inferred from mutant phenotype

(Goyal et al., 2017)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables ATP binding

inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR017441

(InterPro Project Members, 2004-)

enables MAP kinase kinase activity

inferred from biological aspect of ancestor with PANTHER:PTN000684494

(GO Reference Genome Project, 2011-)

enables protein kinase activity

inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR008271

(InterPro Project Members, 2004-)

Biological Process (20 terms)

Terms Based on Experimental Evidence (19 terms)

CV Term

Evidence

References

involved_in border follicle cell migration

inferred from genetic interaction with FLYBASE:sl; FB:FBgn0003416

(Bianco et al., 2007)

involved_in cellular response to X-ray

inferred from mutant phenotype

(Mogila et al., 2006)

involved_in defense response to virus

inferred from mutant phenotype

(Xu et al., 2013)

involved_in determination of adult lifespan

inferred from mutant phenotype

(Slack et al., 2015)

involved_in dorsal/ventral pattern formation

inferred from genetic interaction with FLYBASE:fs(1)K10; FB:FBgn0000810

(Hsu and Perrimon, 1994)

involved_in epidermal growth factor receptor signaling pathway

inferred from genetic interaction with FLYBASE:Egfr; FB:FBgn0003731

(Lim et al., 1997)

inferred from mutant phenotype

(Helman et al., 2011, Zhang et al., 2011, Hsu and Perrimon, 1994)

inferred from genetic interaction with FLYBASE:fs(1)K10; FB:FBgn0000810

(Hsu and Perrimon, 1994)

involved_in ERK1 and ERK2 cascade

inferred from high throughput genetic interaction with FLYBASE:Ras85D; FB:FBgn0003205

(Ashton-Beaucage et al., 2014)

inferred from genetic interaction with FLYBASE:rl; FB:FBgn0003256

(Clemens et al., 2000)

inferred from direct assay

(Lavoie et al., 2018, Douziech et al., 2006, Roy et al., 2002, Oellers and Hafen, 1996)

inferred from mutant phenotype

(Goyal et al., 2017)

involved_in fibroblast growth factor receptor signaling pathway

inferred from mutant phenotype

(Ukken et al., 2014, Wilson et al., 2005)

involved_in hemocyte differentiation

inferred from genetic interaction with FLYBASE:hop; FB:FBgn0004864

(Luo et al., 2002)

involved_in insulin receptor signaling pathway

inferred from direct assay

(Slack et al., 2015, Xu et al., 2013, Kim et al., 2004, Clemens et al., 2000)

involved_in mitotic DNA replication checkpoint signaling

inferred from mutant phenotype

(Mogila et al., 2006)

involved_in mitotic G2 DNA damage checkpoint signaling

inferred from mutant phenotype

(Mogila et al., 2006)

involved_in photoreceptor cell development

inferred from genetic interaction with UniProtKB:P51023

(O'Neill et al., 1994)

involved_in R7 cell fate commitment

inferred from genetic interaction with FLYBASE:Ras85D; FB:FBgn0003205

(Karim et al., 1996)

inferred from mutant phenotype

(Hsu and Perrimon, 1994)

involved_in sevenless signaling pathway

inferred from genetic interaction with FLYBASE:Ras85D; FB:FBgn0003205

(Karim et al., 1996)

inferred from genetic interaction with FLYBASE:sev; FB:FBgn0003366

(Lim et al., 1997)

inferred from mutant phenotype

(Hsu and Perrimon, 1994)

involved_in terminal branching, open tracheal system

inferred from mutant phenotype

(Ukken et al., 2014)

involved_in terminal region determination

inferred from mutant phenotype

(Goyal et al., 2017, Jimenez et al., 2000)

involved_in torso signaling pathway

inferred from genetic interaction with FLYBASE:tor; FB:FBgn0003733

(Lim et al., 1997, Tsuda et al., 1993)

inferred from mutant phenotype

(Goyal et al., 2017)

inferred from genetic interaction with FLYBASE:rl; FB:FBgn0003256

(Lim et al., 1999)

involved_in vascular endothelial growth factor receptor signaling pathway

inferred from direct assay

(Tsuzuki et al., 2014)

inferred from mutant phenotype

(Sopko et al., 2015, Ragab et al., 2011)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in MAPK cascade

inferred from biological aspect of ancestor with PANTHER:PTN000684494

(GO Reference Genome Project, 2011-)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

located_in condensed chromosome

inferred from direct assay

(Mogila et al., 2006)

colocalizes_with nuclear envelope

inferred from direct assay

(Mogila et al., 2006)

Terms Based on Predictions or Assertions (0 terms)

Gene Group (FlyBase)

STE7 MAP KINASE KINASES

Pathway (FlyBase)

INSULIN-LIKE RECEPTOR SIGNALING PATHWAY CORE COMPONENTS

TORSO SIGNALING PATHWAY CORE COMPONENTS

SEVENLESS SIGNALING PATHWAY CORE COMPONENTS

EGFR SIGNALING PATHWAY CORE COMPONENTS

FGFR SIGNALING PATHWAY CORE COMPONENTS

PVR SIGNALING PATHWAY CORE COMPONENTS

Protein Family (UniProt)

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. (Q24324)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

MAP kinase kinase activity

(1) L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+) (2.7.12.2)

(2) L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+) (2.7.12.2)

(3) L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+) (2.7.12.2)

Summaries

Gene Snapshot

Gene Group (FlyBase)

STE7 MAP KINASE KINASES -: STE7 MAP kinase kinases (MAPKKs) belong to the STE7 subfamily and are serine/threonine kinases that phosphorylate MAP kinases in MAPK signalling cascades. MAPKKs are activated by MAP kinase kinase kinases (MAPKKKs). (Adapted from FBrf0225276.)

Pathway (FlyBase)

INSULIN-LIKE RECEPTOR SIGNALING PATHWAY CORE COMPONENTS -: The Insulin-like Receptor (IR) signaling pathway in Drosophila is initiated by the binding of an insulin-like peptides to the Insulin-like receptor (InR). (Adapted from FBrf0232297, FBrf0230017 and FBrf0229989.)
TORSO SIGNALING PATHWAY CORE COMPONENTS -: The formation of Drosophila embryonic termini is controlled by the localized activation of Torso (tor) receptor tyrosine kinase. The Torso signaling pathway acts via the canonical Ras/Raf/MAP kinase cascade. (Adapted from FBrf0157176.)
SEVENLESS SIGNALING PATHWAY CORE COMPONENTS -: The specification of the R7 photoreceptor cell in each ommatidium of the developing Drosophila eye is dependent on activation of Sevenless receptor tyrosine kinase, which acts via the canonical Ras/Raf/MAP kinase cascade to promote the expression of lz and pros. sev, expressed in presumptive R7 cells, is activated by binding to Bride of Sevenless (boss), a seven-transmembrane protein expressed in R8 cells. (Adapted from FBrf0127283 and FBrf0221727).
EGFR SIGNALING PATHWAY CORE COMPONENTS -: The Epidermal Growth Factor Receptor (EGFR) signaling pathway is used multiple times during development (FBrf0190321). It is activated by the binding of a secreted ligand to the receptor tyrosine kinase Egfr and acts via the canonical Ras/Raf/MAP kinase (ERK) cascade. (Adapted from FBrf0190321 and FBrf0221727).
FGFR SIGNALING PATHWAY CORE COMPONENTS -: Fibroblast Growth Factor Receptor (FGFR) signaling pathway is initiated by the binding of secreted FGFs - bnl or ths/pyr to receptor tyrosine kinases btl or htl, respectively, to initiate signaling primarily via the canonical Ras/Raf/MAP kinase (ERK) cascade. (Adapted from FBrf0221038).
PVR SIGNALING PATHWAY CORE COMPONENTS -: PDGF/VEGF (Platelet-Derived Growth Factor/Vascular Endothelial Growth Factor)-receptor related (Pvr) encodes a receptor tyrosine kinase activated by the binding of PDGF- and VEGF-related factors (Pvf1,Pvf2 or Pvf3). Pvr has been shown to activate the canonical Ras/Raf/MAP kinase (ERK) cascade, the PI3K kinase pathway, TORC1 (FBrf0222697), Rho family small GTPases (FBrf0221764, FBrf0180198) and the JNK cascade (FBrf0180198), in a context-dependent manner. (Adapted from FBrf0222697 and FBrf0221727).

Protein Function (UniProtKB)

Required downstream of Raf in the sevenless (sev), torso (tor), and Drosophila EGF receptor homolog (DER) signal transduction pathways. Involved in both positive regulation (at the posterior terminus) and negative regulation (at the anterior domain) of tll, as in other terminal class gene products, maybe via the ERK-A kinase.

(UniProt, Q24324)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Dsor1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q24324)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

PDB - An information portal to biological macromolecular structures

8BW9

Comments on Gene Model

Gene model reviewed during 5.50

Gene model reviewed during 5.55

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Dsor1-RA

FBtr0071313

NM_078543

1799

396

Dsor1-RB

FBtr0340120

NM_001298115

1503

393

Additional Transcript Data and Comments

Reported size (kB)

2.0 (northern blot)

(Tsuda et al., 1993)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Dsor1-PA

FBpp0071248

43.9

396

6.25

FBpp0309113

43.5

393

6.15

X2JAZ3

NP_001285044

AHN59515

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

393 (aa); 44 (kD)

(Tsuda et al., 1993)

Comments

External Data

Subunit Structure (UniProtKB)

Interacts with Raf and ksr; Dsor1 binding to ksr probably promotes ksr and Raf dimerization and ksr-mediated Raf transactivation.

(UniProt, Q24324)

Post Translational Modification

Phosphorylation on Ser/Thr by MAP kinase kinase kinases regulates positively the kinase activity.

(UniProt, Q24324)

Crossreferences

InterPro - A database of protein families, domains and functional sites

PDB - An information portal to biological macromolecular structures

8BW9

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Dsor1 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.76

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism

(Fisher et al., 2012)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

(Tsuda et al., 1993)

Additional Descriptive Data

Dsor1 transcripts are expressed throughout development and are particularly abundant in early embryos.

(Tsuda et al., 1993)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in condensed chromosome

inferred from direct assay

(Mogila et al., 2006)

colocalizes_with nuclear envelope

inferred from direct assay

(Mogila et al., 2006)

Expression Deduced from Reporters

Reporter: TI{CRIMIC.TG4.2}Dsor1^{CR00446-TG4.2}

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult brain | restricted

(Lee et al., 2018)

adult pars intercerebralis | restricted

(Lee et al., 2018)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Dsor1 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Dsor1

View exonic expression by tissue for Dmel\Dsor1

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0010269

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

31872/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0010269

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0010269

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0010269

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG15793

Flygut - An atlas of the Drosophila adult midgut

FBgn0010269

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 31 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 31 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Dsor1

Transgenic constructs containing regulatory region of Dsor1

Aberrations (Deficiencies and Duplications) ( 5 )

Inferred from experimentation ( 5 )

Gene disrupted in

Df(1)10-70d

(Santamaria and Randsholt, 1995)

Df(1)BSC537

(Christensen et al., 2008.6.11)

Df(1)BSC538

(Christensen et al., 2008.6.11)

Df(1)BSC663

(Christensen et al., 2008.12.28)

Df(1)Exel9049

(Ryder, 2004.4.26)

Inferred from location ( 18 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal

lethal, with Scer\GAL4^Mef2.PR

Dsor1^GD9123

lethal | recessive

lethal - all die before end of pupal stage, with Scer\GAL4^ey.PH

Dsor1^GD9123

lethal - all die before end of pupal stage, with Scer\GAL4^pnr-MD237

Dsor1^GD9123

lethal - all die during embryonic stage | recessive

Dsor1^LH110

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Dsor1^GD9123

viable, with Scer\GAL4^tin.CΔ4

Dsor1^GD9123

Sterility

Allele

female sterile, with Scer\GAL4^{VP16.nanos.UTR}, Scer\GAL4^VP16.otu, Scer\GAL4^nanos.PG

Other Phenotypes

Allele

abnormal developmental rate

Dsor1^F72S

abnormal immune response | adult stage, with Scer\GAL4^NP5130

Dsor1^RNAi.UAS.cUa

abnormal neuroanatomy | embryonic stage

Dsor1^LH110

abnormal size | adult stage, with Scer\GAL4^Bx-MS1096

Dsor1^GD9123

decreased body size | adult stage, with Scer\GAL4^Ilp2.PR

decreased cell number | spermatogenesis, with Scer\GAL4^NP1624

Dsor1^HMS00145

decreased occurrence of cell division | embryonic stage | somatic clone

decreased occurrence of cell division | larval stage | somatic clone

embryonic/larval segmentation phenotype, with Scer\GAL4^{VP16.nanos.UTR}, Scer\GAL4^VP16.otu, Scer\GAL4^nanos.PG

Dsor1^F53S.UASp

increased cell death | spermatogenesis, with Scer\GAL4^NP1624

Dsor1^HMS00145

increased cell death | third instar larval stage, with Scer\GAL4^en.PU

Dsor1^GD9123

increased cell number | larval stage

increased occurrence of cell division | spermatogenesis, with Scer\GAL4^bam.PU

Dsor1^HMS00145

increased occurrence of cell division | spermatogenesis, with Scer\GAL4^NP1624

Dsor1^HMS00145

majority die during embryonic stage

Dsor1^E222K

majority die during embryonic stage, with Scer\GAL4^NP1624

Dsor1^Q56P.UASp

majority die during embryonic stage, with Scer\GAL4^{VP16.nanos.UTR}, Scer\GAL4^VP16.otu, Scer\GAL4^nanos.PG

majority die during embryonic stage (with Dsor1^E222K)

Dsor1^F72S

majority die during embryonic stage (with Dsor1^F72S)

Dsor1^E222K

some die during embryonic stage

some die during embryonic stage, with Scer\GAL4^NP1624

Dsor1^G128D.UASp

some die during embryonic stage, with Scer\GAL4^{VP16.nanos.UTR}, Scer\GAL4^VP16.otu, Scer\GAL4^nanos.PG

Dsor1^F53S.UASp

some die during embryonic stage | recessive

Dsor1^LH110

some die during immature adult stage | recessive

Dsor1^XS520

some die during larval stage

Dsor1^F72S

some die during pupal stage, with Scer\GAL4^pnr-MD237

Dsor1^GD9123

visible

Dsor1^XS520

visible, with Scer\GAL4^pnr-MD237

Dsor1^GD9123

visible | adult stage

Dsor1^F72S

visible | adult stage, with Scer\GAL4^Bx-MS1096

Dsor1^GD9123

visible | adult stage, with Scer\GAL4^dpp.blk1

Dsor1^GD9123

visible | adult stage | somatic clone

Dsor1^K8C13

visible | dominant

Dsor1^XS520

visible | recessive

Dsor1^XS520

wild-type

Dsor1^+t9

Phenotype manifest in

Allele

adult heart, with Scer\GAL4^tin.CΔ4

Dsor1^RNAi.UAS.cUa

adult heart muscle cell, with Scer\GAL4^tin.CΔ4

Dsor1^RNAi.UAS.cUa

adult midgut, with Scer\GAL4^NP5130

Dsor1^RNAi.UAS.cUa

chorion, with Scer\GAL4^NP1624

crossvein | ectopic

Dsor1^F72S

cyst cell of testis | absent, with Scer\GAL4^NP1624

Dsor1^HMS00145

cyst progenitor cell, with Scer\GAL4^NP1624

Dsor1^JF03100

cyst progenitor cell | absent, with Scer\GAL4^NP1624

Dsor1^HMS00145

dorsal appendage

dorsal appendage, with Scer\GAL4^NP1624

egg chamber | maternal effect | somatic clone

Dsor1⁷⁵⁶

egg chorion | maternal effect | somatic clone

Dsor1⁷⁵⁶

embryo

Dsor1^F72S

embryo, with Scer\GAL4^{VP16.nanos.UTR}, Scer\GAL4^VP16.otu, Scer\GAL4^nanos.PG

Dsor1^GLV21003

embryo | maternal effect, with Scer\GAL4^{VP16.nanos.UTR}, Scer\GAL4^VP16.otu, Scer\GAL4^nanos.PG

embryonic/larval cuticle, with Scer\GAL4^{VP16.nanos.UTR}, Scer\GAL4^VP16.otu, Scer\GAL4^nanos.PG

embryonic dorsal epidermis

Dsor1^LH110

embryonic head, with Scer\GAL4^NP1624

embryonic head, with Scer\GAL4^{VP16.nanos.UTR}, Scer\GAL4^VP16.otu, Scer\GAL4^nanos.PG

eye

eye photoreceptor cell

Dsor1^XS520

germarium, with Scer\GAL4^unspecified

Dsor1^RNAi.UAS.cUa

germline stem cell, with Scer\GAL4^NP1624

Dsor1^JF03100

imaginal disc | larval stage | somatic clone

intestinal stem cell | adult stage, with Scer\GAL4^NP5130

Dsor1^RNAi.UAS.cUa

male fusome, with Scer\GAL4^bam.PU

Dsor1^HMS00145

male fusome, with Scer\GAL4^NP1624

Dsor1^HMS00145

male germline cell, with Scer\GAL4^bam.PU

Dsor1^HMS00145

male germline cell, with Scer\GAL4^NP1624

Dsor1^JF03100

mesonotum, with Scer\GAL4^pnr-MD237

Dsor1^GD9123

ommatidium

ommatidium | adult stage

photoreceptor cell R1

Dsor1^XS520

photoreceptor cell R2

Dsor1^XS520

photoreceptor cell R3

Dsor1^XS520

photoreceptor cell R4

Dsor1^XS520

photoreceptor cell R5

Dsor1^XS520

photoreceptor cell R6

Dsor1^XS520

photoreceptor cell R7

pigment cell

Dsor1^Su1

spectrosome | increased number, with Scer\GAL4^unspecified

Dsor1^RNAi.UAS.cUa

terminal tracheal cell | ectopic | larval stage

ventral denticle belt | absent | embryonic stage, with Scer\GAL4^NP1624

Dsor1^Q56P.UASp

ventral denticle belt | embryonic stage, with Scer\GAL4^{VP16.nanos.UTR}, Scer\GAL4^VP16.otu, Scer\GAL4^nanos.PG

ventral neurectoderm intermediate zone

Dsor1^LH110

wing, with Scer\GAL4^Bx-MS1096

Dsor1^GD9123

wing disc posterior compartment, with Scer\GAL4^en.PU

Dsor1^GD9123

wing margin | somatic clone

Dsor1^K8C13

wing vein

Dsor1^XS520

wing vein L3, with Scer\GAL4^dpp.blk1

Dsor1^GD9123

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (42)

Hsap\MAP2K1

13 of 14

Yes

Hsap\MAP2K2

12 of 14

Yes

Hsap\LOC407835

7 of 14

Yes

Hsap\MAP2K5

5 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (33)

Rnor\Map2k1

13 of 14

Yes

Rnor\Map2k2

12 of 14

Yes

Rnor\Map2k5

5 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (32)

Mmus\Map2k1

13 of 14

Yes

Mmus\Map2k2

12 of 14

Yes

Mmus\Map2k5

5 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (20)

Xtro\map2k1

10 of 13

Yes

3 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (29)

Drer\map2k1

13 of 14

Yes

Drer\map2k2a

12 of 14

Yes

Drer\map2k2b

12 of 14

Yes

Drer\map2k5

5 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Drer\si:ch211-195b13.1

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (25)

Cele\mek-2

13 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (18)

Agam\AgaP_AGAP001103

11 of 12

Yes

2 of 12

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (42)

Atha\MEK1

11 of 13

Yes

Atha\MKK2

11 of 13

Yes

Atha\MKK6

10 of 13

Yes

Atha\MKK3

7 of 13

Yes

Atha\MKK4

4 of 13

Yes

Atha\MKK5

4 of 13

Yes

Atha\MKK7

3 of 13

Yes

Atha\MKK8

3 of 13

Yes

Atha\MKK9

3 of 13

Yes

Atha\MKK10

3 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Atha\MAPKKK16

1 of 13

Yes

Atha\MAPKKK17

1 of 13

Yes

Atha\MAPKKK18

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (7)

Scer\PBS2

8 of 13

Yes

Scer\MKK1

7 of 13

Yes

Scer\MKK2

7 of 13

Yes

Scer\STE7

5 of 13

Yes

Scer\KIC1

1 of 13

Scer\SPS1

1 of 13

Scer\YPK2

1 of 13

Schizosaccharomyces pombe (Fission yeast) (5)

Spom\byr1

11 of 12

Yes

Spom\pek1

5 of 12

Yes

Spom\wis1

4 of 12

Spom\ppk11

1 of 12

Spom\sid1

1 of 12

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Dsor1. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (18)

lic

4 of 13

Mkk4

4 of 13

hep

2 of 13

Mekk1

2 of 13

Akt

1 of 13

Ask1

1 of 13

CG8173

1 of 13

for

1 of 13

fray

1 of 13

GckIII

1 of 13

hpo

1 of 13

hppy

1 of 13

mbt

1 of 13

msn

1 of 13

Pak3

1 of 13

Slik

1 of 13

Takl1

1 of 13

Tao

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 12 )

Allele

Disease

Evidence

References

Dsor1^F53S.UASp

model of cancer

CEA

(Jindal et al., 2017)

model of RASopathy

CEA

(Marmion et al., 2021)

Dsor1^F53L.UASp

model of cancer

CEA

(Jindal et al., 2017)

Dsor1^E203K.UASp

model of cancer

CEA

(Jindal et al., 2017)

Dsor1^Y130C.UASp

model of cancer

CEA

(Jindal et al., 2017)

Dsor1^D44G.UASp

model of cancer

CEA

(Jindal et al., 2017)

Dsor1^F72S

model of RASopathy

CEA

(Marmion et al., 2023)

Dsor1^Y149C

model of RASopathy

CEA

(Marmion et al., 2023)

Dsor1^E222K

model of RASopathy

CEA

(Marmion et al., 2023)

Dsor1^{F53S.UASp.SSAA}

model of RASopathy

CEA

(Marmion et al., 2021)

Dsor1^F53Y.UASp

model of cancer

CEA

(Andrianova et al., 2023)

Dsor1^G128D.UASp

model of cancer

CEA

(Andrianova et al., 2023)

Dsor1^Q56P.UASp

model of cancer

CEA

(Andrianova et al., 2023)

Potential Models Based on Orthology ( 3 )

Human Ortholog

Disease

Evidence

References

MAP2K1; mitogen-activated protein kinase kinase 1

model of melorheostosis

IEA

(FlyBase, 2019-)

model of cardiofaciocutaneous syndrome 3

IEA

(FlyBase, 2019-)

MAP2K2; mitogen-activated protein kinase kinase 2

model of cardiofaciocutaneous syndrome 4

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 9 )

Allele

Disease

Interaction

References

Dsor1^HMS00037

ameliorates mitochondrial DNA depletion syndrome 15

modeled by TFAM^UAS.cCa

(Duncan et al., 2018)

Dsor1^JF01697

ameliorates mitochondrial DNA depletion syndrome 15

modeled by TFAM^UAS.cCa

(Duncan et al., 2018)

Dsor1^G79

ameliorates autosomal dominant cerebellar ataxia

modeled by Hsap\ATXN1^82Q.UAS

(Park et al., 2013)

Dsor1^LH110

ameliorates pancreatic ductal adenocarcinoma

modeled by CycE^{UAS.Tag:HA.Medsh}, Med^{RNAi.UAS.CycE-HA}, p53^{RNAi.UAS.RasG12D}

(Sekiya et al., 2023)

ameliorates neurofibromatosis

modeled by Nf1^E2

(Walker et al., 2013)

Dsor1^S-1221

ameliorates Huntington's disease

modeled by Hsap\HTT^NT.128Q.UAS

(Miller et al., 2012)

Dsor1^GD9123

ameliorates Huntington's disease

modeled by Hsap\HTT^NT.128Q.UAS

(Miller et al., 2012)

ameliorates neurofibromatosis

modeled by Nf1^E2

(Walker et al., 2013)

Dsor1^KK102276

ameliorates hematologic cancer

modeled by mxc¹

(Kurihara et al., 2020)

ameliorates neurofibromatosis

modeled by Nf1^E2

(Walker et al., 2013)

ameliorates colorectal cancer

modeled by SH3PX1^JF02730

(Zhang et al., 2019)

Dsor1^F72S

model of RASopathy

is ameliorated by grk^unspecified

(Marmion et al., 2023)

Dsor1^RNAi.UAS.cUa

ameliorates cardiomyopathy

modeled by Hsap\RAF1^gof.UAS

(Yu et al., 2013)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

MAP2K1; mitogen-activated protein kinase kinase 1

13 of 14

176872

MAP2K2; mitogen-activated protein kinase kinase 2

12 of 14

601263

CARDIOFACIOCUTANEOUS SYNDROME 4; CFC4

cardiofaciocutaneous syndrome 4

Hsap\LOC407835

7 of 14

MAP2K5; mitogen-activated protein kinase kinase 5

5 of 14

602520

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Dsor1 - 14-3-3ε

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Dsor1 - 14-3-3ζ

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Dsor1 - Akap200

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Dsor1 - AspRS-m

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Dsor1 - CG12948

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Dsor1 - CG1416

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Dsor1 - CG4972

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Dsor1 - CSN8

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Dsor1 - Dp1

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Dsor1 - Droj2

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Dsor1 - GCS2β

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Dsor1 - Gmppb

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Dsor1 - Golgin97

experimental knowledge based

(Guruharsha et al., 2011)

Dsor1 - HDAC1

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Dsor1 - Hsp83

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Dsor1 - Imp

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Dsor1 - Lamtor3

pull down, autoradiography

(Mouchel-Vielh et al., 2008)

Dsor1 - Map60

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Dsor1 - Mbs

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Dsor1 - Mcad

anti tag coimmunoprecipitation, anti tag western blot

(Friedman et al., 2011)

Dsor1 - Mcm7

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Dsor1 - Mec2

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Dsor1 - Mkk4

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Dsor1 - Nepl3

experimental knowledge based

(Guruharsha et al., 2011)

Dsor1 - Ostm1

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Dsor1 - Pyk

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Dsor1 - Raf

anti tag coimmunoprecipitation, peptide massfingerprinting, two hybrid, anti tag western blot

(Maisonneuve et al., 2024, Friedman et al., 2011, Therrien et al., 1998)

Dsor1 - Rbcn-3A

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Dsor1 - RfC4

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Dsor1 - RhoGAP18B

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Dsor1 - RpL10

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Dsor1 - RpL15

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Dsor1 - RpL24

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Dsor1 - RpL28

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Dsor1 - RpL32

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Dsor1 - RpL9

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Dsor1 - RpS2

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Dsor1 - RpS20

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Dsor1 - RpS28b

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Dsor1 - RpS30

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Dsor1 - Rpt4

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Dsor1 - Rpt6

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Dsor1 - SERCA

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Dsor1 - Saf-B

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Dsor1 - ScsβA

anti tag coimmunoprecipitation, peptide massfingerprinting, anti tag western blot

(Friedman et al., 2011, Friedman et al., 2011)

Dsor1 - ScsβG

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Dsor1 - Sec13

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Dsor1 - SkpA

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Dsor1 - UQCR-C2

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Dsor1 - ZAP3

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Dsor1 - arm

proximity ligation assay, fluorescence microscopy

(Hall and Verheyen, 2015)

Dsor1 - ave

pull down, anti tag western blot, electron microscopy, molecular sieving, molecular weight estimation by staining

(Maisonneuve et al., 2024)

Dsor1 - blanks

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Dsor1 - brm

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Dsor1 - caz

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Dsor1 - cindr

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Dsor1 - cnk

pull down, anti tag western blot, molecular sieving, molecular weight estimation by staining, anti tag coimmunoprecipitation, peptide massfingerprinting, electron microscopy

(Maisonneuve et al., 2024, Friedman et al., 2011)

Dsor1 - cpa

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Dsor1 - eIF3a

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Dsor1 - eIF3l

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Dsor1 - eIF4E1

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Dsor1 - glo

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Dsor1 - krz

anti tag coimmunoprecipitation, anti tag western blot

(Tipping et al., 2010)

Dsor1 - ksr

electron microscopy, anti tag coimmunoprecipitation, peptide massfingerprinting, anti tag western blot, molecular sieving, molecular weight estimation by staining

(Maisonneuve et al., 2024, Friedman et al., 2011)

Dsor1 - kuk

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Dsor1 - larp

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Dsor1 - nesd

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Dsor1 - ptch

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Dsor1 - rl

anti tag coimmunoprecipitation, western blot, proximity ligation assay, fluorescence microscopy

(Hall and Verheyen, 2015, Tipping et al., 2010)

Dsor1 - scny

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Dsor1 - sesB

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Dsor1 - sip2

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Dsor1 - sqd

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Dsor1 - vlc

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Dsor1 - vret

experimental knowledge based

(Guruharsha et al., 2011)

Dsor1 - wal

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Dsor1 - αTub84D

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Dsor1 - βCOP

anti tag coimmunoprecipitation, anti tag western blot

(Friedman et al., 2011)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Dsor1

suppressible

aop

(Karim et al., 1996)

enhanceable

(Yu et al., 2021)

enhanceable

(Taguchi et al., 2000)

Dsor1

suppressible

cnk

(Zhang et al., 1999)

Dsor1

suppressible

Egfr

(Zhang et al., 1999)

Dsor1

suppressible

ER2-1

(Karim et al., 1996)

Dsor1

suppressible

ER2-3

(Karim et al., 1996)

Dsor1

suppressible

ER2-4

(Karim et al., 1996)

Dsor1

enhanceable

ER2-7B

(Karim et al., 1996)

Dsor1

suppressible

ER3-2

(Karim et al., 1996)

Dsor1

enhanceable

ER3-4

(Karim et al., 1996)

Dsor1

suppressible

grk

(Marmion et al., 2023)

Dsor1

enhanceable

(Karim et al., 1996)

Dsor1

enhanceable

ksr

(Karim et al., 1996)

Dsor1

enhanceable

mam

(Karim et al., 1996)

Dsor1

suppressible

mts

(Karim et al., 1996)

Dsor1

enhanceable

pnt

(O'Neill et al., 1994)

Dsor1

suppressible

PTP-ER

(Karim and Rubin, 1999, Karim et al., 1996)

Dsor1

suppressible

(Kushnir et al., 2020, Lim et al., 1999, Zhang et al., 1999)

Dsor1|fs(1)M3

enhanceable

(Lim et al., 1999)

Dsor1

enhanceable

(Karim et al., 1996)

Dsor1

enhanceable

SR3-6

(Karim et al., 1996)

Dsor1

suppressible

Src42A

(Lu and Li, 1999, Lu et al., 1994)

suppressible

(Lu et al., 1994)

suppressible

(Lu et al., 1994)

suppressible

(Lu et al., 1994)

suppressible

(Lu et al., 1994)

suppressible

(Lu et al., 1994)

suppressible

(Zhang et al., 1999)

Dsor1

enhanceable

Taf6

(Karim et al., 1996)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

aos

suppressible

Dsor1

(Sawamoto et al., 1996)

asp

suppressible

Dsor1

(Inoue and Glover, 1998)

Atg8a

suppressible

Dsor1

(Zhang et al., 2019)

aux

suppressible

Dsor1

(Zhao et al., 2022)

csw

suppressible

Dsor1

(Oishi et al., 2006, Tsuda et al., 1993)

suppressible

(Lim et al., 1997)

suppressible

(Lim et al., 1999)

suppressible

(Kurada and White, 1998, Sawamoto et al., 1998)

hid

enhanceable

Dsor1

(Kurada and White, 1998)

hop

suppressible

Dsor1

(Luo et al., 2002)

suppressible

Dsor1

(Baker et al., 2002)

ksr::tor

enhanceable

Dsor1

(Therrien et al., 2000)

lace

suppressible

Dsor1

(Adachi-Yamada et al., 1999)

suppressible

Dsor1

(Nagaraj et al., 1999)

Nf1

suppressible

Dsor1

(Walker et al., 2013)

suppressible

(Lee et al., 2002)

enhanceable

(Lim et al., 1997)

suppressible

(Lim et al., 1997, Hsu and Perrimon, 1994, Lu et al., 1994, Tsuda et al., 1993)

Ras85D

suppressible

Dsor1

(Zhang et al., 2024, Zeng et al., 2010, Therrien et al., 2000, Karim et al., 1996, O'Neill et al., 1994)

Ras85D|p53|CycE|Med

suppressible

Dsor1

(Sekiya et al., 2023)

rpr

suppressible

Dsor1

(Kurada and White, 1998)

enhanceable

(Kim et al., 2006)

suppressible

(Lim et al., 1997)

suppressible

(Zhang et al., 2019)

Src42A

suppressible

Dsor1

(Takahashi et al., 1996)

svp

suppressible

Dsor1

(Kramer et al., 1995)

Syx17

suppressible

Dsor1

(Zhang et al., 2019)

tor

suppressible

Dsor1

(Li and Li, 2003, Lim et al., 1997)

External Data

Subunit Structure (UniProtKB)

Interacts with Raf and ksr; Dsor1 binding to ksr probably promotes ksr and Raf dimerization and ksr-mediated Raf transactivation.

(UniProt, Q24324 )

Linkouts

BioGRID - A database of protein and genetic interactions.

58322

DroID - A comprehensive database of gene and protein interactions.

FBgn0010269

MIST (genetic) - An integrated Molecular Interaction Database

31872

MIST (protein-protein) - An integrated Molecular Interaction Database

31872

Pathways

Signaling Pathways (FlyBase)

INSULIN-LIKE RECEPTOR SIGNALING PATHWAY CORE COMPONENTS
TORSO SIGNALING PATHWAY CORE COMPONENTS
SEVENLESS SIGNALING PATHWAY CORE COMPONENTS
EGFR SIGNALING PATHWAY CORE COMPONENTS
FGFR SIGNALING PATHWAY CORE COMPONENTS
PVR SIGNALING PATHWAY CORE COMPONENTS

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q24324

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-27

Cytogenetic map

8D2-8D3

Sequence location

X:9,247,342..9,250,037 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

8D2-8D3

Limits computationally determined from genome sequence between P{EP}EP1450&P{EP}EP1356 and P{EP}EP912^EP912

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

8D-8D

(Nishida, 1992.11.27)

8D-8D

(determined by in situ hybridisation)

(Tsuda et al., 1993)

Experimentally Determined Recombination Data

Location

1-27

(FlyBase, 2016)

1-28.4

(Comeron et al., 2012)

1-27.6

(Nishida, 1992.11.27)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (23)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

28685

y¹ v¹; P{TRiP.JF03100}attP2

31184

y¹ v¹; P{TRiP.JF01697}attP2

33639

y¹ v¹; P{TRiP.HMS00037}attP2

8496

y¹ w^a Dsor1^S-1221/Basc

7136

w¹ Dsor1^G79/FM7c/Dp(1;Y)FF1, y⁺

7131

y¹ ac¹ z¹ Dsor1^G42/FM7a

FlyORF

F001292

M{UAS-Dsor1.ORF}ZH-86Fb

F001169

M{UAS-Dsor1.ORF.3xHA}ZH-86Fb

11153

v107276

v40026

w¹¹¹⁸; P{GD9123}v40026

v342006

P{hsFLP}1, y¹ w¹¹¹⁸; P{HD_CFD01356}attP40/CyO-GFP

More stocks available...

Genomic Clones (7)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (19)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD41207

Other clones

DPiM_LD41207

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0010269

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

LD05838

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0010269

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

dsRNA made from templates generated with primers directed against this gene.

(Douziech et al., 2006)

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

The MAPK cascade is required for Ras85D mitogenic response, loss of function mutations in phl, Dsor1, rl and ksr dominantly suppress hyperplastic growth, as do mutations in the Ras85D effector loop that disrupt the Ras85D-phl interaction.

(Karim and Rubin, 1998)

Downstream signal transducer of torso, sevenless and DER receptors.

(Noselli and Glise, 1996)

Studies of interaction between argos and members of the Ras/MAPK pathway demonstrate the argos gene product is a negative regulator of signal transduction that acts upstream of the Ras/MAPK cascade.

(Sawamoto et al., 1996)

Expression of Dsor1 in lin-3, let-23 or lin-45 C.elegans mutants permits P5.p, P6.p and P7.p segments to adopt the vulval fates in each of the vulvaless backgrounds.

(Koga and Ohshima, 1995)

Additional alleles of Dsor1 have been isolated in screen for lethal mutations that fail to complement Df(1)10-70d.

(Santamaria and Randsholt, 1995)

A screen to identify mutations affecting the Ras85D signalling pathway identified alleles of phl, Dsor1, rl, aop, βggt-I, mts, ksr and phyl.

(Therrien et al., 1995)

Down-regulation of bcd activity depends on the function of Dsor1. Dsor1 acts downstream of phl in the tor pathway and encodes a MAP-kinase kinase (MAPKK). Several clustered consensus sites for MAP kinase phosphorylation can be found in the bcd coding sequence.

(Dostatni et al., 1994)

Dsor1 acts downstream of phl in the DER pathway.

(Hsu and Perrimon, 1994)

Dsor1 and phl both play a role in the determination of the rate of proliferation. Maternally derived Dsor1 is required for development of the embryonic termini. Dsor1 is involved in both the positive and negative regulation of tll.

(Tsuda et al., 1993)

Relationship to Other Genes

Source for database merge of

Additional comments

Wild-type Dsor1 does not complement the mating defect of S.cerevisiae Scer\ste7 mutants. A mutated form of Dsor1 (carrying the lesion found in Dsor1^Su1) does complement the mating defect of S.cerevisiae Scer\ste7 mutants. Wild-type Dsor1 does not complement the temperature-sensitive cell-lysis defect of S.cerevisiae Scer\MKK1 Scer\MKK2 double mutants. A mutated form of Dsor1 (carrying the lesion found in Dsor1^Su1) does complement the temperature-sensitive cell-lysis defect of S.cerevisiae Scer\MKK1 Scer\MKK2 double mutants. Wild-type Dsor1 or a mutated form of Dsor1 (carrying the lesion found in Dsor1^Su1) does not complement the sensitivity to osmotic pressure of S.cerevisiae Scer\pbs2 mutants.

(Lim et al., 1997)

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (47)

Reported As

Symbol Synonym

CG15793

(Hosono et al., 2015, Fukuoh et al., 2014, Cui et al., 2013, Schertel et al., 2013, Ni et al., 2011.7.19, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Perkins et al., 2009, Mouchel-Vielh et al., 2008, Ni et al., 2008, Morrison et al., 2000)

D-MEK

(Zhang et al., 2011, Hermon et al., 1998)

D-MEK/Dsor

(Baek and Lee, 1999)

D-Mek

(Perrimon et al., 1995)

D-SOR

(Martin-Blanco, 2000)

D-Sor

(Yang et al., 2018, Sackton et al., 2007)

D-mek

(Morisato and Anderson, 1995, Hsu and Perrimon, 1994, Lu et al., 1994)

D-sor

(Janody et al., 2000)

D-sor-1

(Perrimon and Desplan, 1994)

DMEK-1

(Duffy and Perrimon, 1994)

DRODSOR1

(Fernando Vazquez et al., 2000, Lopez et al., 1999, Schug et al., 1997)

DSOR

(Das and Arur, 2017)

DSOR1

(Park et al., 2013, Clemens et al., 2000, Raabe, 2000)

DSORT

(Claeys et al., 2002)

DSor

(Ip and Davis, 1998, Therrien et al., 1995)

DSor1

(Wilson et al., 2005)

Dmek

(Mantrova and Hsu, 1998)

Dsor1

(Fukuda et al., 2024, Koehler et al., 2024, Rodríguez-Martín et al., 2024, Andrianova et al., 2023, Sekiya et al., 2023, Perlegos et al., 2022, Zhao et al., 2022, Bilder et al., 2021, DeAngelis et al., 2021, Fabian et al., 2021, Harnish et al., 2021, Khan et al., 2021, Lebo et al., 2021, Marmion et al., 2021, Nguyen et al., 2021, Ostalé et al., 2021, Yu et al., 2021, Zheng et al., 2021, Alameh et al., 2020, Graves et al., 2020, Hayashi and Ogura, 2020, Kierdorf et al., 2020, Kurihara et al., 2020, Wang et al., 2020, Chai et al., 2019, Fenckova et al., 2019, Zirin et al., 2019, Das et al., 2018, Du et al., 2018, Gene Disruption Project members, 2018-, Inoue et al., 2018, Lee et al., 2018, Levis, 2018.8.30, Muñoz-Soriano et al., 2018, Ashton-Beaucage and Therrien, 2017, Goyal et al., 2017, Houtz et al., 2017, Jindal et al., 2017, Lusk et al., 2017, Song et al., 2017, Transgenic RNAi Project members, 2017-, Ashton-Beaucage et al., 2016, Malartre, 2016, Hall and Verheyen, 2015, Li et al., 2015, Zang et al., 2015, Chuang et al., 2014, Gonzalez et al., 2014, Hasygar and Hietakangas, 2014, Kim et al., 2014, Sopko et al., 2014, Bergwitz et al., 2013, Carter, 2013, Chen et al., 2013, Cui et al., 2013, Mbodj et al., 2013, Park et al., 2013, Schertel et al., 2013, Walker et al., 2013, Yamamoto et al., 2013-, Yin et al., 2013, Gomez et al., 2012, Legent et al., 2012, Miller et al., 2012, Friedman et al., 2011, Friedman et al., 2011, Friedman et al., 2011, Maher et al., 2011, Ragab et al., 2011, Zeng et al., 2010, Hill-Burns and Clark, 2009, Sims et al., 2009, Christensen et al., 2008.6.11, Christensen et al., 2008.6.11, Christensen et al., 2008.12.28, Mouchel-Vielh et al., 2008, Xia et al., 2008, Zhou et al., 2008, Nishimura et al., 2007, Nishimura et al., 2007, Guichard et al., 2006, Mogila et al., 2006, Oishi et al., 2006, Walker et al., 2006, Michellod et al., 2003, Dawes-Hoang and Wieschaus, 2001, Lim et al., 1997)

EK1-1

(Therrien et al., 2000)

MAPKK

(Lusk et al., 2017, Herr et al., 2010, Bianco et al., 2007, Yoneda et al., 2007, Dawes-Hoang and Wieschaus, 2001, Kumar and Moses, 2000, Marshall, 1994)

MEK

(Maisonneuve et al., 2024, Ureña et al., 2024, Yang et al., 2024, Andrianova et al., 2023, Sekiya et al., 2023, Zhang et al., 2022, Øvrebø et al., 2022, Marmion et al., 2021, Valon et al., 2021, Koyama et al., 2020, Mariano et al., 2020, Paul et al., 2020, Sun et al., 2020, Johnson and Toettcher, 2019, Wittes and Schüpbach, 2019, Zhang et al., 2019, Inoue et al., 2018, Lavoie et al., 2018, Goyal et al., 2017, Lusk et al., 2017, Jin et al., 2015, Sopko et al., 2015, Pichaud, 2014, Bergwitz et al., 2013, Yu et al., 2013, Miller et al., 2012, Herr et al., 2010, Majumdar et al., 2010, Roignant and Treisman, 2010, Tipping et al., 2010, Nie et al., 2009, Mouchel-Vielh et al., 2008, Ashton-Beaucage and Therrien, 2007, Mogila et al., 2007, Douziech et al., 2006, Kim et al., 2006, Mogila and Li, 2006, Mogila et al., 2006, Vrailas et al., 2006, Walker et al., 2006, Harrison et al., 2005, Douziech et al., 2003, Anselmo et al., 2002, Castelli-Gair Hombria and Brown, 2002, Rebay, 2002, Roy and Therrien, 2002, Roy et al., 2002, Yokokura et al., 2002, Kumar and Moses, 2001, Kumar and Moses, 2001, Morrison et al., 2000, Duffy et al., 1998, Karim and Rubin, 1998, Lu and Li, 1998, Perrimon and Duffy, 1998, Karim et al., 1997, Badenhorst et al., 1996, Klämbt et al., 1996, Avruch et al., 1994)

MEK/Dsor

(Baldenius et al., 2023)

MEK/Dsor1

(Friedman and Perrimon, 2006)

MEK1/2

(Su et al., 2019, Ivanovska et al., 2004)

Mek

(Sawyer et al., 2020, Kumar and Moses, 2000)

Mek1/2

(Billmann et al., 2018)

SOR

(Morrison et al., 2000)

Sor

(Kim et al., 2006)

Su(Raf)34B

(Zhang et al., 1999)

dMEK

(Kim et al., 2004, Lee et al., 2002)

dMek

(Swevers et al., 2018)

dSor

(Lee et al., 2002)

dSor1

(Majumdar et al., 2010)

dsor

(Datta et al., 2023)

dsor1

(Colonnetta et al., 2021, Banisch et al., 2017, Pae et al., 2017, Moodie and Wolfman, 1994)

mek

(Ma et al., 2019, Selcho et al., 2017, Ashton-Beaucage et al., 2016, Ashton-Beaucage et al., 2014, Ukken et al., 2014, Ashton-Beaucage et al., 2010, Liu et al., 2010, Therrien et al., 2000)

sor

(Morrison et al., 2000)

sor/MEK1

(Wilson et al., 2001)

Name Synonyms

Downstream of Raf1

(Hall and Verheyen, 2015)

Downstream of raf1

(Graves et al., 2020, Young and Marcotte, 2017, Malartre, 2016, Muha and Müller, 2013, Legent et al., 2012, Oishi et al., 2006)

Drosophila MEK

(Mogila et al., 2007)

MAP Kinase Kinase

(Majumdar et al., 2010)

MAPK kinase

(Bianco et al., 2007)

Map kinase kinase

(Harrison et al., 2005)

dSor1

(Kraut, 2011)

downstream suppressor of Raf 1

(Xia et al., 2008)

mitogen-activated protein kinase kinase

(Yu et al., 2013)

Secondary FlyBase IDs

Datasets (1)

Study focus (1)

Experimental Role

Project

Project Type

Title

bait_protein

RTK-Ras-ERK_interaction_map

interactome

Interaction map generated by purification of receptor tyrosine kinase pathway factors, with identification of copurifying proteins by mass spectrometry.

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 125 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

31872

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q24324

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q24324

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

X2JAZ3

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q24324

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0010269

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

31872/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0010269

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0010269

FlyMine - An integrated database for Drosophila genomics

FBgn0010269

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG15793

MARRVEL_MODEL - MARRVEL (model organism gene)

31872

PDB - An information portal to biological macromolecular structures

8BW9

Linkouts

BioGRID - A database of protein and genetic interactions.

58322

Cell Signaling Technology - Commercial vendor for primary antibodies and antibody conjugates.

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0010269

DroID - A comprehensive database of gene and protein interactions.

FBgn0010269

DRSC - Results frm RNAi screens

FBgn0010269

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0010269

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0010269

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG15793

Flygut - An atlas of the Drosophila adult midgut

FBgn0010269

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC004803

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

MIST (genetic) - An integrated Molecular Interaction Database

31872

MIST (protein-protein) - An integrated Molecular Interaction Database

31872

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q24324

References (349)

The following external sites may use different assemblies or annotations than FlyBase.

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