FlyBase curator comment: this entry is used to capture phenotypic information when the particular allele (or allele combination) used by the author could not be determined but the context of the experiment suggests that the phenotype being described is some kind of loss of function.
Germaria with germ line grkunspecified mutation do not exhibit ectopic spectrosome containing cells.
grkunspecified mutant oocytes, of a similar size to wild-type size 8 exhibit dramatically disrupted microtubule distribution. Specifically, microtubule organisation appears completely reversed compared to wild-type, in which the nucleus is at the anterior and microtubule plue-ends are located towards the posterior at stage 8. In slightly older oocytes, microtubules remain stretched out along the cortex from the posterior toward the anterior, where they fold back to the center of the oocyte. Microtubule distribution in grkunspecified oocytes is strikingly similar to microtubules of wild-type egg chambers before the migration of the oocyte nucleus. Likewise, the centrosome, found at the posterior of stage 6 wild-type oocytes, stays at the posterior in grkunspecified mutants. Thus, in grkunspecified mutants, the distribution of microtubules and the microtubule organising center seems similar to their distribution in wild-type stage 6.
After cold-shock treatment of grkunspecified mutant oocytes, microtubule depolymerisation occurs in an asymmetric pattern and is restricted to the areas surrounding the nucleus, as in wild-type.
grkunspecified is a suppressor | partially of some die during embryonic stage phenotype of Dsor1F72S
grkunspecified/grk[+] is a suppressor of border follicle cell | somatic clone phenotype of CblF165
Df(3L)Krn-27-7-B, grkunspecified, spi1 has germarium phenotype
Tl3, gd7, grkunspecified has embryonic epidermis phenotype
Germaria with germ line double mutant for grkunspecified and spi1 do not exhibit ectopic spectrosome containing cells.
Germaria with germ line triple mutant for Df(3L)Krn-27-7-B, grkunspecified and spi1 exhibit ectopic spectrosome containing cells.
Defective migration of border follicle cells in CblF165 homozygous somatic clones is suppressed by grkunspecified/+.
Embryos from gd7/gd7;Tl3/+ mothers are lateralized. When the mothers are also duplicated for dpp, embryos show a more strongly lateralized phenotype with a more dorsal character - fewer, finer or absent denticles and a lateralized gastrulation pattern where the germband does not extend dorsally and no polarity is visible. Cuticles of embryos collected from gd7/gd7;grkunspecified;Tl3 mothers have a more ventral character than those derived from gd7/gd7;Tl3 mothers.
Cuticles of embryos collected from gd7/gd7;grkunspecified;Tl3 have a more ventral character than those derived from gd7/gd7;Tl3 mothers.
grkunspecified is rescued by grk+t9
