FlyBase Allele Report: Dmel\Tl[3]

General Information

Symbol

Dmel\Tl³

Species

D. melanogaster

Name

FlyBase ID

FBal0016828

Feature type

allele

Associated gene

Dmel\Tl

Associated Insertion(s)

Carried in Construct

Also Known As

Tl^9Q, Toll^9Q

Key Links

Genomic Maps

JBrowse

Allele class

gain of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

gain of function allele

(Lemaitre et al., 1996, Lemaitre et al., 1995, Ray et al., 1991, Schneider et al., 1991)

Mutagen

ethyl methanesulfonate

(Tearle and Nusslein-Volhard, 1987, Anderson et al., 1985)

Progenitor genotype

Cytology

Polytene chromosomes normal.

(Anderson et al., 1985)

Description

Amino acid replacement.

(Lemaitre et al., 1995)

Point mutation in the open reading frame.

(Hecht and Anderson, 1993)

Nucleotide substitution: G2970A. Amino acid replacement: C799Y. This mutation is in the extracellular domain of the protein.

(Schneider et al., 1991)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:26,840,249..26,840,249 [+]

Nucleotide change:

G26840249A

Reported nucleotide change:

G2970A

Amino acid change:

C799Y | Tl-PB; C799Y | Tl-PC; C799Y | Tl-PD

Reported amino acid change:

C799Y

Comment:

This mutation is in the extracellular domain of the protein.

(Schneider et al., 1991)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal immune response

(Kim et al., 2006)

female sterile | dominant

(Hecht and Anderson, 1993)

female sterile germline-dependent | dominant

(Kerridge and Thomas-Cavallin, 1988)

lethal | dominant | maternal effect

(Gerttula et al., 1988)

lethal | recessive

(Anderson et al., 1985)

Phenotype Manifest In

Detailed Description

Statement

Reference

Females heterozygous for Tl³ produce progeny with cuticles bearing rings of ventral denticles.

(Stein et al., 2010)

Embryos generated from Tl³/+ mothers are ventralized.

(Carneiro et al., 2006)

Embryos derived from Tl³/+ mothers show strongly ventralized cuticles.

(Chen et al., 2006)

Tl³ mutants exhibit melanotic masses.

(Kim et al., 2006)

Heterozygous third instar larvae have melanotic nodules found in the hemocoel or in association with He-positive lymph glands. The melanotic nodules are surrounded by lamellocytes.

(Minakhina and Steward, 2006)

Embryos derived from mutant mothers are strongly ventralized.

(Araujo and Bier, 2000)

Embryos derived from heterozygous females are ventralised.

(Jazwinska et al., 1999)

Less than 1% of heterozygous adults have melanotic capsules.

(Qiu et al., 1998)

In the absence of immune challenge the antifungal gene Drs is constitutively expressed.

(Lemaitre et al., 1996)

Abnormal fat body morphology. P{Dipt2.2-lacZ} is not induced in heterozygous larvae.

(Lemaitre et al., 1995)

Cell intercalation in lateralized Tl mutant embryos proceeds normally during germ band extension.

(Irvine and Wieschaus, 1994)

Dominant ventralising phenotype.

(Hecht and Anderson, 1993)

Ventralized embryos: rings or patches of ventral denticles along dorsoventral axis. Altered pattern of dpp and zen.

(Ray et al., 1991)

Embryos from heterozygous females are strongly ventralised; the ventral epidermis is expanded, there is complete loss of dorsal epidermis and the mesoderm is not expanded. Females carrying Tl³ in trans with a null allele of Tl produce ventralised embryos.

(Schneider et al., 1991)

Embryos derived from Tl³ females are ventralised. Axons are in diffuse and disorganised arrays.

(Smouse and Perrimon, 1990)

dominant

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Tl³ has abnormal immune response phenotype, non-suppressible by casp^UAS.Tag:MYC/Scer\GAL4^yolk

(Kim et al., 2006)

NOT Enhancer of

Statement

Reference

Tl³ is a non-enhancer of visible phenotype of upd1^GMR.PB

(Bach et al., 2003)

NOT Suppressor of

Statement

Reference

Tl³ is a non-suppressor of visible phenotype of upd1^GMR.PB

(Bach et al., 2003)

Other

Statement

Reference

Tl³, cact³/cact[+] has melanotic mass phenotype phenotype

(Qiu et al., 1998)

Tl[+]/Tl³, cact³ has melanotic mass phenotype phenotype

(Qiu et al., 1998)

Phenotype Manifest In

Enhanced by

Statement

Reference

Tl³, gd⁷ has embryonic epidermis phenotype, enhanceable by Dp(2;1)G146

(Araujo and Bier, 2000)

NOT Enhanced by

Statement

Reference

Tl³ has embryonic/first instar larval cuticle | maternal effect phenotype, non-enhanceable by sel^f04257/sel^f04257

(Stein et al., 2010)

Suppressed by

Statement

Reference

Tl³ has embryonic dorsal epidermis phenotype, suppressible by Myd88^kra-1

(Charatsi et al., 2003)

Dp(2;1)G146, Tl³ has embryonic epidermis phenotype, suppressible | heat sensitive | maternal effect | partially by dpp^BP.hs

(Araujo and Bier, 2000)

Tl³ has embryonic epidermis phenotype, suppressible by Dp(2;1)G146

(Araujo and Bier, 2000)

NOT suppressed by

Statement

Reference

Tl³ has embryonic/first instar larval cuticle | maternal effect phenotype, non-suppressible by sel^f04257/sel^f04257

(Stein et al., 2010)

NOT Enhancer of

Statement

Reference

Tl³ is a non-enhancer of eye phenotype of upd1^GMR.PB

(Bach et al., 2003)

Tl³ is a non-enhancer of embryonic epidermis | ventral phenotype of Myd88^kra-1

(Charatsi et al., 2003)

NOT Suppressor of

Statement

Reference

Tl³ is a non-suppressor of eye phenotype of upd1^GMR.PB

(Bach et al., 2003)

Other

Statement

Reference

Tl³, wek^EX14/wek^lor has embryonic/first instar larval cuticle | lateral phenotype

(Chen et al., 2006)

Myd88^kra-1, Tl³ has embryonic epidermis | ventral phenotype

(Charatsi et al., 2003)

Tl³, gd⁷ has embryonic epidermis phenotype

(Araujo and Bier, 2000)

Tl³, gd⁷, grk^unspecified has embryonic epidermis phenotype

(Araujo and Bier, 2000)

Tl³, snk²/snk⁴ has embryonic/first instar larval cuticle phenotype

(Neul and Ferguson, 1998)

Tl³, cact³/cact[+] has melanotic mass phenotype

(Qiu et al., 1998)

Tl[+]/Tl³, cact³ has melanotic mass phenotype

(Qiu et al., 1998)

Additional Comments

Genetic Interactions

Statement

Reference

Females heterozygous for Tl³which are also homozygous for sel^f04257 produce progeny with cuticles bearing rings of ventral denticles, like the progeny of females carrying Tl³ alone.

(Stein et al., 2010)

Embryos produced by wek^lor/wek^EX14; Tl³/+ mothers show a biphasic phenotype distribution. 76% show a moderately dorsalized phenotype; these embryos lack ventral and lateral structures but retain the dorsolaterally-derived filzkorper. 24% show a lateralized phenotype; these embryos are elongated and thin in appearance and contain fine rings of laterally-derived ventral denticle belts.

(Chen et al., 2006)

Overexpression of casp^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^yolk fails to suppress the ectopic melanisation phenotype of Tl³ mutants.

(Kim et al., 2006)

Embryos laid by homozygous Myd88^kra-1 and Tl³/+ mothers show a completely dorsalised phenotype.

(Charatsi et al., 2003)

13% of embryos from Dp(2;1)G146 mothers show weakly ventralised, as opposed to strongly ventralised, mutant phenotype. The penetrance of this suppression is increased to 50-80% when dpp dosage in increased with dpp^BP.hs.

Embryos from gd⁷/gd⁷;Tl³/+ mothers are lateralized. When the mothers are also duplicated for dpp, embryos show a more strongly lateralized phenotype with a more dorsal character - fewer, finer or absent denticles and a lateralized gastrulation pattern where the germband does not extend dorsally and no polarity is visible.

Cuticles of embryos collected from gd⁷/gd⁷;grk^unspecified;Tl³ have a more ventral character than those derived from gd⁷/gd⁷;Tl³ mothers.

13% of embryos from mutant mothers show weakly ventralised, as opposed to strongly ventralised, mutant phenotype. The penetrance of this suppression is increased to 50-80% when dpp dosage in increased with dpp^BP.hs. Embryos from gd⁷/gd⁷;Tl³/+ mothers are lateralized. When the mothers are also duplicated for dpp, embryos show a more strongly lateralized phenotype with a more dorsal character - fewer, finer or absent denticles and a lateralized gastrulation pattern where the germband does not extend dorsally and no polarity is visible. Cuticles of embryos collected from gd⁷/gd⁷;grk^unspecified;Tl³ have a more ventral character than those derived from gd⁷/gd⁷;Tl³ mothers.

(Araujo and Bier, 2000)

Embryos derived from snk⁴ Tl³/snk² + females differentiate cuticles encircled by ventral denticles. Injection of these embryos with tkv^Q253D.SP6 RNA restores dorsal structures in a dose-dependent manner. Injection of these embryos with sax^Q263D.SP6 RNA has little or no effect; none of these embryos develop amnioserosa and only 2% differentiate dorsal hairs. Injection of these embryos with sax^Q263D.SP6 and tkv^wt.SP6 RNA does not promote the formation of any dorsal structures. Injection of these embryos with both tkv^Q253D.SP6 and sax^Q263D.SP6 RNA results in a striking increase in the percentage of embryos that differentiate dorsal tissue types compared to embryos injected with the same concentration of tkv^Q253D.SP6 RNA alone. Injection of these embryos with both tkv^Q253D.SP6 and sax^wt.SP6 RNA does not increase the formation of dorsal structures compared to embryos injected with tkv^Q253D.SP6 RNA alone. Injection of these embryos with both tkv^Q253D.SP6 and tkv^DN.SP6 RNA does not increase the formation of dorsal structures compared to embryos injected with tkv^Q253D.SP6 RNA alone.

(Neul and Ferguson, 1998)

43% of cact³/+ ; Tl³/+ adults have melanotic capsules.

(Qiu et al., 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Class I allele.

(Schneider et al., 1991)

Class I Tl allele.

(Hecht and Anderson, 1993)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

Tl³

(Fontenele et al., 2009, Davis et al., 2008, Carneiro et al., 2006, Kim et al., 2006, Mizutani et al., 2006)

Tl^9Q38

(Kerridge and Thomas-Cavallin, 1988)

Tl^9q

(Unlu and Minden, 1998)

Tl^D

(Lemaitre et al., 1996)

Toll³

(Minakhina and Steward, 2006)

Toll^9Q

(Chen et al., 2006, Charatsi et al., 2003, Jazwinska et al., 1999)

Toll^D

(Neumann and Cohen, 1997)

Name Synonyms

Toll9Q

(Stein et al., 2010)

Secondary FlyBase IDs

References (41)

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