FlyBase Allele Report: Dmel\sel[f04257]

General Information

Symbol

Dmel\sel^f04257

Species

D. melanogaster

Name

FlyBase ID

FBal0187411

Feature type

allele

Associated gene

Dmel\sel

Associated Insertion(s)

PBac{WH}sel^f04257

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

piggyBac activity

Nature of the Allele

Allele class

Mutagen

piggyBac activity

(Gene Disruption Project members and Exelixis, 2005)

Progenitor genotype

Associated Insertion(s)

PBac{WH}sel^f04257

Cytology

Description

Allele components

Component

Use(s)

Inserted element

PBac{WH}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Also carries

FRT

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:10,051,387..10,051,387 [-]

(Gene Disruption Project members and Exelixis, 2005)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

filzkorper | maternal effect

(Stein et al., 2010)

ventral denticle belt | maternal effect

(Stein et al., 2010)

Detailed Description

Statement

Reference

60% of embryonic cuticles produced by females homozygous for sel^f04257 exhibit the moderately severe phenotype: ventral denticles are of narrower than normal width while the dorsally derived tracheal structures referred to as 'Filzkorper' are present. 39% of cuticles show the strongly dorsalized phenotype: they lack denticles but produce Filzkorper. Fewer than 1% of embryos are completely dorsalized, lacking both ventral denticles and Filzkorper. Fewer than 1% of embryos display the weakest phenotype: ventral denticle bands of normal width and Filzkorper are present, but embryos exhbit a tail-up or twisted phenotype.

Embryos produced by sel^f04257 females exhibit appropriately polarized gastrulation movements and fail to show ventral mesoderm.

(Stein et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

sel^f04257/sel^f04257 is a non-enhancer of embryonic/first instar larval cuticle | maternal effect phenotype of Tl³

(Stein et al., 2010)

NOT Suppressor of

Statement

Reference

sel^f04257/sel^f04257 is a non-suppressor of embryonic/first instar larval cuticle | maternal effect phenotype of Tl³

(Stein et al., 2010)

Other

Statement

Reference

gd^UASp.mGFP6, sel^f04257/CG12918[+] has embryonic/first instar larval cuticle | maternal effect phenotype

(Stein et al., 2010)

gd^UASp.mGFP6, sel^f04257 has embryonic/first instar larval cuticle | maternal effect phenotype

(Stein et al., 2010)

sel^f04257/CG12918[+], snk^{ΔN.UASp.mGFP6} has embryonic/first instar larval cuticle | maternal effect phenotype

(Stein et al., 2010)

sel^f04257, snk^{ΔN.UASp.mGFP6} has embryonic/first instar larval cuticle | maternal effect phenotype

(Stein et al., 2010)

ea^{ΔN.UASp.mGFP6}, sel^f04257/CG12918[+] has embryonic/first instar larval cuticle | maternal effect phenotype

(Stein et al., 2010)

ea^{ΔN.UASp.mGFP6}, sel^f04257 has embryonic/first instar larval cuticle | maternal effect phenotype

(Stein et al., 2010)

sel^f04257/CG12918[+], spz^{ΔN.UASp.mGFP6} has embryonic/first instar larval cuticle | maternal effect phenotype

(Stein et al., 2010)

sel^f04257, spz^{ΔN.UASp.mGFP6} has embryonic/first instar larval cuticle | maternal effect phenotype

(Stein et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Females heterozygous for Tl³which are also homozygous for sel^f04257 produce progeny with cuticles bearing rings of ventral denticles, like the progeny of females carrying Tl³ alone.

Scer\GAL4^{nos.UTR.T:Hsim\VP16}-mediated expression of spz^{ΔN.P\T.Scer\UAS.T:Avic\GFP-m6} in either sel^f04257/+ or sel^f04257/sel^f04257 females leads to the formation of lateralized progeny embryos.

Scer\GAL4^{nos.UTR.T:Hsim\VP16}-mediated expression of snk^{ΔN.Scer\UAS.P\T.T:Avic\GFP-m6} or ea^{ΔN.P\T.Scer\UAS.T:Avic\GFP-m6} in sel^f04257/+ females leads to the formation of apolar, lateralized embryos, whereas expressing either transgene in sel^f04257/sel^f04257 females produces strongly dorsalized progeny.

Scer\GAL4^{nos.UTR.T:Hsim\VP16}-mediated expression of gd^{Scer\UAS.P\T.T:Avic\GFP-m6} in sel^f04257/+ females leads to the formation of lateralized and ventralized embryos, whereas expressing this transgene in sel^f04257/sel^f04257 females produces dorsalized progeny.

(Stein et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Injection of in vitro synthesized RNA encoding the sel ORF rescues the progeny of sel^f04257/Df(2R)X3 females.

(Stein et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

86096

w¹¹¹⁸; PBac{WH}sel^f04257

Notes on Origin

Discoverer

Separable from: PBac{WH}CG8552^f04257.

(Gene Disruption Project members and Exelixis, 2005)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

CG12918^f04257

sel^f04257

(Stein et al., 2010)

Name Synonyms

seelef04257

(Stein et al., 2010)

Secondary FlyBase IDs

References (2)

Report Sections

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