FlyBase Allele Report: Dmel\Dsor1[GD9123]

General Information

Symbol

Dmel\Dsor1^GD9123

Species

D. melanogaster

Name

FlyBase ID

FBal0206911

Feature type

allele

Associated gene

Dmel\Dsor1

Associated Insertion(s)

Carried in Construct

P{GD9123}

Also Known As

UAS-Dsor^RNAi, Dsor1^RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD9123}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD9123

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Huntington's disease

modeled by Hsap\HTT^NT.128Q.UAS

(Miller et al., 2012)

ameliorates neurofibromatosis

modeled by Nf1^E2

(Walker et al., 2013)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | adult stage, with Scer\GAL4^Bx-MS1096

(Kushnir et al., 2020)

decreased body size | adult stage, with Scer\GAL4^Ilp2.PR

(Hasygar and Hietakangas, 2014)

increased cell death | third instar larval stage, with Scer\GAL4^en.PU

(Hall and Verheyen, 2015)

lethal, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

lethal - all die before end of pupal stage, with Scer\GAL4^ey.PH

(Kushnir et al., 2020)

lethal - all die before end of pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

some die during pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

visible | adult stage, with Scer\GAL4^Bx-MS1096

(Kushnir et al., 2020)

visible | adult stage, with Scer\GAL4^dpp.blk1

(Hall and Verheyen, 2015)

Phenotype Manifest In

mesonotum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

wing, with Scer\GAL4^Bx-MS1096

(Kushnir et al., 2020)

wing disc posterior compartment, with Scer\GAL4^en.PU

(Hall and Verheyen, 2015)

wing vein L3, with Scer\GAL4^dpp.blk1

(Hall and Verheyen, 2015)

Detailed Description

Statement

Reference

Expressing Dsor1^GD9123 under the control of Scer\GAL4^Bx-MS1096 results in underdeveloped vestigial wings.

(Kushnir et al., 2020)

Expressing Dsor1^GD9123 under the control of Scer\GAL4^en.PU induces cell death in the posterior compartment of the wing disc, while expression under the control of Scer\GAL4^dpp.blk1 results in loss of vein material in wing vein L3.

(Hall and Verheyen, 2015)

Scer\GAL4^Ilp2.PR-mediated expression results in a significant reduction in the body weight of emerging adults compared to controls.

(Hasygar and Hietakangas, 2014)

Adults expressing Dsor1^GD9123 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in adult/pharate lethality or late pupal lethality.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 may result in lethality, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 70-80% of the Scer\GAL4^pnr-MD237-expressing area of the notum.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

9123

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Dsor1^GD9123, Scer\GAL4^Bx-MS1096 has abnormal size | adult stage phenotype, suppressible | partially by rl^{R80S.D334N.UAS}, Scer\GAL4^Bx-MS1096

(Kushnir et al., 2020)

Dsor1^GD9123, Scer\GAL4^Bx-MS1096 has abnormal size | adult stage phenotype, suppressible | partially by rl^{D334N.UAS.cKa}, Scer\GAL4^Bx-MS1096

(Kushnir et al., 2020)

Dsor1^GD9123, Scer\GAL4^Bx-MS1096 has visible | adult stage phenotype, suppressible | partially by rl^R80S.UAS, Scer\GAL4^Bx-MS1096

(Kushnir et al., 2020)

Dsor1^GD9123, Scer\GAL4^Bx-MS1096 has visible | adult stage phenotype, suppressible | partially by rl^{D334N.UAS.cKa}, Scer\GAL4^Bx-MS1096

(Kushnir et al., 2020)

Dsor1^GD9123, Scer\GAL4^Bx-MS1096 has visible | adult stage phenotype, suppressible | partially by rl^{R80S.D334N.UAS}, Scer\GAL4^Bx-MS1096

(Kushnir et al., 2020)

Dsor1^GD9123, Scer\GAL4^ey.PH has increased mortality during development phenotype, suppressible | partially by rl^WT.UAS, Scer\GAL4^ey.PH

(Kushnir et al., 2020)

Dsor1^GD9123, Scer\GAL4^Bx-MS1096 has abnormal size | adult stage phenotype, suppressible | partially by rl^R80S.UAS, Scer\GAL4^Bx-MS1096

(Kushnir et al., 2020)

Dsor1^GD9123, Scer\GAL4^en.PU has increased cell death | third instar larval stage phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^en.PU

(Hall and Verheyen, 2015)

NOT suppressed by

Statement

Reference

Dsor1^GD9123, Scer\GAL4^Bx-MS1096 has abnormal size | adult stage phenotype, non-suppressible by rl^WT.UAS, Scer\GAL4^Bx-MS1096

(Kushnir et al., 2020)

Dsor1^GD9123, Scer\GAL4^Bx-MS1096 has visible | adult stage phenotype, non-suppressible by rl^WT.UAS, Scer\GAL4^Bx-MS1096

(Kushnir et al., 2020)

NOT Enhancer of

Statement

Reference

Dsor1^GD9123, Scer\GAL4^GMR.PU is a non-enhancer of visible phenotype of Hsap\LRRK2^UAS.cCa, Scer\GAL4^GMR.PU, grim^GMR.PU

(Chuang et al., 2014)

Suppressor of

Statement

Reference

Scer\GAL4^nSyb.PS/Dsor1^GD9123 is a suppressor of decreased body size | pupal stage phenotype of Nf1^E2

(Walker et al., 2013)

Other

Statement

Reference

Dsor1^GD9123, Scer\GAL4^ey.PH, rl^{D334N.UAS.cKa} has partially lethal - majority die phenotype

(Kushnir et al., 2020)

Dsor1^GD9123, Scer\GAL4^ey.PH, rl^{D334N.UAS.cKa} has visible | adult stage phenotype

(Kushnir et al., 2020)

Dsor1^GD9123, Scer\GAL4^ey.PH, rl^{R80S.D334N.UAS} has partially lethal - majority live phenotype

(Kushnir et al., 2020)

Dsor1^GD9123, Scer\GAL4^ey.PH, rl^{R80S.D334N.UAS} has visible | adult stage phenotype

(Kushnir et al., 2020)

Dsor1^GD9123, Scer\GAL4^ey.PH, rl^WT.UAS has partially lethal - majority die phenotype

(Kushnir et al., 2020)

Dsor1^GD9123, Scer\GAL4^ey.PH, rl^WT.UAS has visible | adult stage phenotype

(Kushnir et al., 2020)

Dsor1^GD9123, Scer\GAL4^ey.PH, rl^WT.UAS has abnormal size | adult stage phenotype

(Kushnir et al., 2020)

Dsor1^GD9123, Scer\GAL4^ey.PH, rl^R80S.UAS has partially lethal - majority die phenotype

(Kushnir et al., 2020)

Dsor1^GD9123, Scer\GAL4^ey.PH, rl^R80S.UAS has visible | adult stage phenotype

(Kushnir et al., 2020)

Dsor1^GD9123, Scer\GAL4^en.PU, rl[+]/rl^Sem has visible | adult stage phenotype

(Hall and Verheyen, 2015)

Dsor1^GD9123, Nf1^E2, Scer\GAL4^Ras64B.PW has lethal phenotype

(Walker et al., 2013)

Dsor1^GD9123, Nf1^E2, Scer\GAL4^C23 has lethal phenotype

(Walker et al., 2013)

Phenotype Manifest In

Suppressed by

Statement

Reference

Dsor1^GD9123, Scer\GAL4^Bx-MS1096 has wing phenotype, suppressible | partially by rl^R80S.UAS, Scer\GAL4^Bx-MS1096

(Kushnir et al., 2020)

Dsor1^GD9123, Scer\GAL4^Bx-MS1096 has wing phenotype, suppressible | partially by rl^{D334N.UAS.cKa}, Scer\GAL4^Bx-MS1096

(Kushnir et al., 2020)

Dsor1^GD9123, Scer\GAL4^Bx-MS1096 has wing phenotype, suppressible | partially by rl^{R80S.D334N.UAS}, Scer\GAL4^Bx-MS1096

(Kushnir et al., 2020)

Dsor1^GD9123, Scer\GAL4^en.PU has wing disc posterior compartment phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^en.PU

(Hall and Verheyen, 2015)

NOT suppressed by

Statement

Reference

Dsor1^GD9123, Scer\GAL4^Bx-MS1096 has wing phenotype, non-suppressible by rl^WT.UAS, Scer\GAL4^Bx-MS1096

(Kushnir et al., 2020)

NOT Enhancer of

Statement

Reference

Dsor1^GD9123, Scer\GAL4^GMR.PU is a non-enhancer of eye phenotype of Hsap\LRRK2^UAS.cCa, Scer\GAL4^GMR.PU, grim^GMR.PU

(Chuang et al., 2014)

Dsor1^GD9123, Scer\GAL4^GMR.PU is a non-enhancer of ommatidium phenotype of Hsap\LRRK2^UAS.cCa, Scer\GAL4^GMR.PU, grim^GMR.PU

(Chuang et al., 2014)

Other

Statement

Reference

Dsor1^GD9123, Scer\GAL4^ey.PH, rl^{R80S.D334N.UAS} has eye phenotype

(Kushnir et al., 2020)

Dsor1^GD9123, Scer\GAL4^ey.PH, rl^WT.UAS has eye phenotype

(Kushnir et al., 2020)

Dsor1^GD9123, Scer\GAL4^ey.PH, rl^R80S.UAS has eye phenotype

(Kushnir et al., 2020)

Dsor1^GD9123, Scer\GAL4^ey.PH, rl^{D334N.UAS.cKa} has eye phenotype

(Kushnir et al., 2020)

Dsor1^GD9123, Scer\GAL4^en.PU, rl[+]/rl^Sem has wing phenotype

(Hall and Verheyen, 2015)

Dsor1^GD9123, Scer\GAL4^en.PU, rl[+]/rl^Sem has wing vein phenotype

(Hall and Verheyen, 2015)

Additional Comments

Genetic Interactions

Statement

Reference

The vestigial wing phenotype induced by the expression of Dsor1^GD9123 under the control of Scer\GAL4^Bx-MS1096 is considerably suppressed by the co-expression of rl^{R80S.D334N.UAS}, but only subtly by the co-expression of either rl^R80S.UAS or rl^{D334N.UAS.cKa}.

The pupal lethality of individuals expressing Dsor1^GD9123 under the control of Scer\GAL4^ey.PH is partially rescued, although with an associated small eye phenotype, by the co-expression of rl^WT.UAS (few escapers, with mild-to-severe eye phenotype), rl^R80S.UAS (majority die, mostly mild eye phenotype), rl^{D334N.UAS.cKa} (majority die, mostly mild eye phenotype), rl^{R80S.D334N.UAS} (majority live, mostly mild eye phenotype).

(Kushnir et al., 2020)

Expressing Dsor1^GD9123 under the control of Scer\GAL4^en.PU in a rl^Sem/+ background results in notched wings with a mix of ectopic and lost vein tissue. Wings did not show creases, or the "droopy" phenotype.

(Hall and Verheyen, 2015)

Expression of Dsor1^GD9123 under the control of Scer\GAL4^nSyb.PS suppresses the reduced pupal length phenotype seen in Nf1^E2 animals.

(Walker et al., 2013)

Xenogenetic Interactions

Statement

Reference

Co-expression of Dsor1^GD9123 does not reverse the suppression of the grim^GMR.PU eye phenotype seen when Hsap\LRRK2^Scer\UAS.cCa is expressed under the control of Scer\GAL4^GMR.PU.

(Chuang et al., 2014)

Complementation and Rescue Data

Rescued by

Dsor1^GD9123 is rescued by Scer\GAL4^dpp.blk1/Dsor1^UAS.ORF

(Hall and Verheyen, 2015)

Comments

Dsor1^UAS.ORF under the control of Scer\GAL4^dpp.blk1 rescues the loss of wing material in wing vein L3 in flies co-expressing Dsor1^GD9123.

(Hall and Verheyen, 2015)

Images (0)

Mutant

Wild-type

Stocks (1)