FlyBase Allele Report: Dmel\grim[GMR.PU]

General Information

Symbol

Dmel\grim^GMR.PU

Species

D. melanogaster

Name

glass multimer reporter construct of Unknown

FlyBase ID

FBal0299623

Feature type

allele

Associated gene

Dmel\grim

Associated Insertion(s)

Carried in Construct

P{GMR-grim.U}

Also Known As

GMR-grim

Key Links

Transgenic product class

wild_type

(FlyBase Curators, 2025-)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(FlyBase Curators, 2025-)

Mutagen

in vitro construct

(FlyBase Curators, 2025-)

Progenitor genotype

Carried in construct

P{GMR-grim.U}

Cytology

Description

GMR regulatory sequences drive expression of grim.

(FlyBase Curators, 2025-)

FlyBase curator comment: this entry represents a transgenic construct where the particular construct used cannot be determined.

(FlyBase Curators, 2025-)

Allele components

Component

Use(s)

Regulatory region(s)

GMR

engineered transcription regulatory region

Encoded product / tool

grim

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible

(Hwangbo et al., 2016, Chuang et al., 2014)

Phenotype Manifest In

eye

(Hwangbo et al., 2016, Chuang et al., 2014)

ommatidium

(Chuang et al., 2014)

Detailed Description

Statement

Reference

grim^GMR.PU leads to small eyes with apoptotic phenotypes in the retina.

(Hwangbo et al., 2016)

Expression of grim^GMR.PU results in a reduced eye size compared to controls and severe disruption to the ommatidia.

(Chuang et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Hsap\LRRK2^UAS.cCa, Scer\GAL4^GMR.PU, grim^GMR.PU has visible phenotype, enhanceable by foxo^UAS.cPa, Scer\GAL4^GMR.PU

(Chuang et al., 2014)

Hsap\LRRK2^UAS.cCa, Scer\GAL4^GMR.PU, grim^GMR.PU has visible phenotype, enhanceable by Akt^RNAi.UAS.cUa, Scer\GAL4^GMR.PU

(Chuang et al., 2014)

Hsap\LRRK2^UAS.cCa, Scer\GAL4^GMR.PU, grim^GMR.PU has visible phenotype, enhanceable by foxo^{S259A.UAS.cUa}, Scer\GAL4^GMR.PU

(Chuang et al., 2014)

NOT Enhanced by

Statement

Reference

Hsap\LRRK2^UAS.cCa, Scer\GAL4^GMR.PU, grim^GMR.PU has visible phenotype, non-enhanceable by hep^GD1461, Scer\GAL4^GMR.PU

(Chuang et al., 2014)

Hsap\LRRK2^UAS.cCa, Scer\GAL4^GMR.PU, grim^GMR.PU has visible phenotype, non-enhanceable by Dsor1^GD9123, Scer\GAL4^GMR.PU

(Chuang et al., 2014)

Hsap\LRRK2^UAS.cCa, Scer\GAL4^GMR.PU, grim^GMR.PU has visible phenotype, non-enhanceable by sgg^S9A.UAS.cUa, Scer\GAL4^GMR.PU

(Chuang et al., 2014)

Hsap\LRRK2^UAS.cCa, Scer\GAL4^GMR.PU, grim^GMR.PU has visible phenotype, non-enhanceable by Pi3K21B^GD9810, Scer\GAL4^GMR.PU

(Chuang et al., 2014)

Hsap\LRRK2^UAS.cCa, Scer\GAL4^GMR.PU, grim^GMR.PU has visible phenotype, non-enhanceable by rl^GD12910, Scer\GAL4^GMR.PU

(Chuang et al., 2014)

Suppressed by

Statement

Reference

grim^GMR.PU has visible phenotype, suppressible | partially by DCAF12^GD10933/Scer\GAL4^GMR.PU

(Hwangbo et al., 2016)

grim^GMR.PU has visible phenotype, suppressible | partially by Hsap\LRRK2^UAS.cCa/Scer\GAL4^GMR.PU

(Chuang et al., 2014)

Hsap\LRRK2^UAS.cCa, Scer\GAL4^GMR.PU, grim^GMR.PU has visible phenotype, suppressible by Akt^UAS.Exel, Scer\GAL4^GMR.PU

(Chuang et al., 2014)

Hsap\LRRK2^{G2019S.UAS.cLa.Tag:FLAG}, Scer\GAL4^GMR.PU, grim^GMR.PU has visible phenotype, suppressible by Akt^{T342D.S505D.GMR}

(Chuang et al., 2014)

Hsap\LRRK2^{R1441C.UAS.Tag:FLAG}, Scer\GAL4^GMR.PU, grim^GMR.PU has visible phenotype, suppressible by Akt^{T342D.S505D.GMR}, Scer\GAL4^GMR.PU

(Chuang et al., 2014)

NOT suppressed by

Statement

Reference

grim^GMR.PU has visible phenotype, non-suppressible by Hsap\LRRK2^{G2019S.UAS.cLa.Tag:FLAG}/Scer\GAL4^GMR.PU

(Chuang et al., 2014)

grim^GMR.PU has visible phenotype, non-suppressible by Hsap\LRRK2^{R1441C.UAS.Tag:FLAG}/Scer\GAL4^GMR.PU

(Chuang et al., 2014)

Phenotype Manifest In

Enhanced by

Statement

Reference

Hsap\LRRK2^UAS.cCa, Scer\GAL4^GMR.PU, grim^GMR.PU has eye phenotype, enhanceable by Akt^RNAi.UAS.cUa, Scer\GAL4^GMR.PU

(Chuang et al., 2014)

Hsap\LRRK2^UAS.cCa, Scer\GAL4^GMR.PU, grim^GMR.PU has ommatidium phenotype, enhanceable by Akt^RNAi.UAS.cUa, Scer\GAL4^GMR.PU

(Chuang et al., 2014)

Hsap\LRRK2^UAS.cCa, Scer\GAL4^GMR.PU, grim^GMR.PU has eye phenotype, enhanceable by foxo^UAS.cPa, Scer\GAL4^GMR.PU

(Chuang et al., 2014)

Hsap\LRRK2^UAS.cCa, Scer\GAL4^GMR.PU, grim^GMR.PU has ommatidium phenotype, enhanceable by foxo^UAS.cPa, Scer\GAL4^GMR.PU

(Chuang et al., 2014)

Hsap\LRRK2^UAS.cCa, Scer\GAL4^GMR.PU, grim^GMR.PU has eye phenotype, enhanceable by foxo^{S259A.UAS.cUa}, Scer\GAL4^GMR.PU

(Chuang et al., 2014)

Hsap\LRRK2^UAS.cCa, Scer\GAL4^GMR.PU, grim^GMR.PU has ommatidium phenotype, enhanceable by foxo^{S259A.UAS.cUa}, Scer\GAL4^GMR.PU

(Chuang et al., 2014)

NOT Enhanced by

Statement

Reference

Hsap\LRRK2^UAS.cCa, Scer\GAL4^GMR.PU, grim^GMR.PU has ommatidium phenotype, non-enhanceable by hep^GD1461, Scer\GAL4^GMR.PU

(Chuang et al., 2014)

Hsap\LRRK2^UAS.cCa, Scer\GAL4^GMR.PU, grim^GMR.PU has eye phenotype, non-enhanceable by Dsor1^GD9123, Scer\GAL4^GMR.PU

(Chuang et al., 2014)

Hsap\LRRK2^UAS.cCa, Scer\GAL4^GMR.PU, grim^GMR.PU has ommatidium phenotype, non-enhanceable by Dsor1^GD9123, Scer\GAL4^GMR.PU

(Chuang et al., 2014)

Hsap\LRRK2^UAS.cCa, Scer\GAL4^GMR.PU, grim^GMR.PU has eye phenotype, non-enhanceable by sgg^S9A.UAS.cUa, Scer\GAL4^GMR.PU

(Chuang et al., 2014)

Hsap\LRRK2^UAS.cCa, Scer\GAL4^GMR.PU, grim^GMR.PU has ommatidium phenotype, non-enhanceable by sgg^S9A.UAS.cUa, Scer\GAL4^GMR.PU

(Chuang et al., 2014)

Hsap\LRRK2^UAS.cCa, Scer\GAL4^GMR.PU, grim^GMR.PU has eye phenotype, non-enhanceable by Pi3K21B^GD9810, Scer\GAL4^GMR.PU

(Chuang et al., 2014)

Hsap\LRRK2^UAS.cCa, Scer\GAL4^GMR.PU, grim^GMR.PU has ommatidium phenotype, non-enhanceable by Pi3K21B^GD9810, Scer\GAL4^GMR.PU

(Chuang et al., 2014)

Hsap\LRRK2^UAS.cCa, Scer\GAL4^GMR.PU, grim^GMR.PU has eye phenotype, non-enhanceable by rl^GD12910, Scer\GAL4^GMR.PU

(Chuang et al., 2014)

Hsap\LRRK2^UAS.cCa, Scer\GAL4^GMR.PU, grim^GMR.PU has ommatidium phenotype, non-enhanceable by rl^GD12910, Scer\GAL4^GMR.PU

(Chuang et al., 2014)

Hsap\LRRK2^UAS.cCa, Scer\GAL4^GMR.PU, grim^GMR.PU has eye phenotype, non-enhanceable by hep^GD1461, Scer\GAL4^GMR.PU

(Chuang et al., 2014)

Suppressed by

Statement

Reference

grim^GMR.PU has eye phenotype, suppressible | partially by DCAF12^GD10933/Scer\GAL4^GMR.PU

(Hwangbo et al., 2016)

Hsap\LRRK2^{G2019S.UAS.cLa.Tag:FLAG}, Scer\GAL4^GMR.PU, grim^GMR.PU has eye phenotype, suppressible by Akt^{T342D.S505D.GMR}

(Chuang et al., 2014)

Hsap\LRRK2^{G2019S.UAS.cLa.Tag:FLAG}, Scer\GAL4^GMR.PU, grim^GMR.PU has ommatidium phenotype, suppressible by Akt^{T342D.S505D.GMR}

(Chuang et al., 2014)

Hsap\LRRK2^{R1441C.UAS.Tag:FLAG}, Scer\GAL4^GMR.PU, grim^GMR.PU has eye phenotype, suppressible by Akt^{T342D.S505D.GMR}, Scer\GAL4^GMR.PU

(Chuang et al., 2014)

Hsap\LRRK2^{R1441C.UAS.Tag:FLAG}, Scer\GAL4^GMR.PU, grim^GMR.PU has ommatidium phenotype, suppressible by Akt^{T342D.S505D.GMR}, Scer\GAL4^GMR.PU

(Chuang et al., 2014)

grim^GMR.PU has eye phenotype, suppressible | partially by Hsap\LRRK2^UAS.cCa/Scer\GAL4^GMR.PU

(Chuang et al., 2014)

grim^GMR.PU has ommatidium phenotype, suppressible | partially by Hsap\LRRK2^UAS.cCa/Scer\GAL4^GMR.PU

(Chuang et al., 2014)

Hsap\LRRK2^UAS.cCa, Scer\GAL4^GMR.PU, grim^GMR.PU has eye phenotype, suppressible by Akt^UAS.Exel, Scer\GAL4^GMR.PU

(Chuang et al., 2014)

Hsap\LRRK2^UAS.cCa, Scer\GAL4^GMR.PU, grim^GMR.PU has ommatidium phenotype, suppressible by Akt^UAS.Exel, Scer\GAL4^GMR.PU

(Chuang et al., 2014)

NOT suppressed by

Statement

Reference

grim^GMR.PU has eye phenotype, non-suppressible by Hsap\LRRK2^{G2019S.UAS.cLa.Tag:FLAG}/Scer\GAL4^GMR.PU

(Chuang et al., 2014)

grim^GMR.PU has ommatidium phenotype, non-suppressible by Hsap\LRRK2^{G2019S.UAS.cLa.Tag:FLAG}/Scer\GAL4^GMR.PU

(Chuang et al., 2014)

grim^GMR.PU has eye phenotype, non-suppressible by Hsap\LRRK2^{R1441C.UAS.Tag:FLAG}/Scer\GAL4^GMR.PU

(Chuang et al., 2014)

grim^GMR.PU has ommatidium phenotype, non-suppressible by Hsap\LRRK2^{R1441C.UAS.Tag:FLAG}/Scer\GAL4^GMR.PU

(Chuang et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of DCAF12^GD10933 driven by Scer\GAL4^GMR.PU partially suppresses apoptotic eye phenotypes in grim^GMR.PU flies.

(Hwangbo et al., 2016)

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\LRRK2^Scer\UAS.cCa under the control of Scer\GAL4^GMR.PU partially suppresses the eye phenotypes seen upon expression of grim^GMR.PU.

Co-expression of Akt1^VDRC.cUa reverses the suppression of the grim^GMR.PU eye phenotype seen when Hsap\LRRK2^Scer\UAS.cCa is expressed under the control of Scer\GAL4^GMR.PU.

Co-expression of Akt1^{Scer\UAS.Exel} enhances the suppression of the grim^GMR.PU eye phenotype seen when Hsap\LRRK2^Scer\UAS.cCa is expressed under the control of Scer\GAL4^GMR.PU.

Co-expression of Pi3K21B^GD9810 does not reverse the suppression of the grim^GMR.PU eye phenotype seen when Hsap\LRRK2^Scer\UAS.cCa is expressed under the control of Scer\GAL4^GMR.PU.

Co-expression of rl^GD12910 does not reverse the suppression of the grim^GMR.PU eye phenotype seen when Hsap\LRRK2^Scer\UAS.cCa is expressed under the control of Scer\GAL4^GMR.PU.

Co-expression of hep^GD1461 does not reverse the suppression of the grim^GMR.PU eye phenotype seen when Hsap\LRRK2^Scer\UAS.cCa is expressed under the control of Scer\GAL4^GMR.PU.

Co-expression of Dsor1^GD9123 does not reverse the suppression of the grim^GMR.PU eye phenotype seen when Hsap\LRRK2^Scer\UAS.cCa is expressed under the control of Scer\GAL4^GMR.PU.

Co-expression of foxo^Scer\UAS.cPa reverses the suppression of the grim^GMR.PU eye phenotype seen when Hsap\LRRK2^Scer\UAS.cCa is expressed under the control of Scer\GAL4^GMR.PU.

Co-expression of foxo^{S259A.Scer\UAS.cUa} reverses the suppression of the grim^GMR.PU eye phenotype seen when Hsap\LRRK2^Scer\UAS.cCa is expressed under the control of Scer\GAL4^GMR.PU.

Co-expression of sgg^{S9A.Scer\UAS.cUa} does not reverse the suppression of the grim^GMR.PU eye phenotype seen when Hsap\LRRK2^Scer\UAS.cCa is expressed under the control of Scer\GAL4^GMR.PU.

Expression of Hsap\LRRK2^{G2019S.Scer\UAS.cLa.T:Zzzz\FLAG} under the control of Scer\GAL4^GMR.PU does not significantly suppress the eye phenotypes seen upon expression of grim^GMR.PU.

Expression of Hsap\LRRK2^{R1441C.Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^GMR.PU does not significantly suppress the eye phenotypes seen upon expression of grim^GMR.PU.

Expression of a constitutively active form of Akt1 (Akt1^{T342D.S505D.GMR}) significantly rescues the eye phenotype seen when grim^GMR.PU is co-expressed with Hsap\LRRK2^{G2019S.Scer\UAS.cLa.T:Zzzz\FLAG} (under the control of Scer\GAL4^GMR.PU).

Expression of a constitutively active form of Akt1 (Akt1^{T342D.S505D.GMR}) significantly rescues the eye phenotype seen when grim^GMR.PU is co-expressed with Hsap\LRRK2^{R1441C.Scer\UAS.T:Zzzz\FLAG} (under the control of Scer\GAL4^GMR.PU).

(Chuang et al., 2014)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

grim^GMR.PU

Name Synonyms

glass multimer reporter construct of Unknown

Secondary FlyBase IDs

References (5)

Report Sections

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