FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000049768
Interaction Network
Interactions Browser links
ksr network
Dsor1 network
Reported Interactions
FBrf0224702-129.coIP.MS
Description
physical association
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Dsor1

bait

TAP tag

ksr 

ksr

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

Overall SAINT probability of interaction = 1.

FBrf0224702-248.coIP.MS
Description
physical association
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ksr 

ksr

bait

TAP tag

Dsor1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

Overall SAINT probability of interaction = 1.

FBrf0260095-11.CH.MW
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ksr 

Ksr

neutral component

His-GST tag

ksr 
ksr 

Ksr

neutral component

His-GST tag

ksr 
ksr 

Ksr

neutral component

His-GST tag

MEK

neutral component

MEK

neutral component

MEK

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ksr 
Kinase domain
sufficient binding region
aa 654-966
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Purified ave-cnk heterodimer was mixed with an equimolar ratio of ksr-Dsor1 heterodimer, which eluted as a higher order complex as series of binary interactions that do not necessarily indicate direct protein-protein contact. Mutations in cnk or ksr interfere with the higher order cnk-ave-ksr-Dsor1 complex.

Interaction in vitro; proteins produced as a recombinant fusion proteins in baculovirus and insect cell system.

FBrf0260095-16.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ksr 

Ksr

bait

V5 tag

ksr 
ksr 

MEK

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ksr 
C-lobe base domain residue 903
mutation decreasing interaction
aa 903

within the context of 654-966; M903D; coordinates relative to ksr-PA

ksr 
C-lobe base domain residue 896
mutation decreasing interaction
aa 896

within the context of 654-966; W896R; coordinates relative to ksr-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Co-expression of cnk and ave increase ksr-Dsor1 interaction.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0260095-19.EM
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ksr 

Ksr

neutral component

His-GST tag

ksr 

Ksr

neutral component

His-GST tag

ksr 
ksr 

Ksr

neutral component

His-GST tag

ksr 

MEK

neutral component

MEK

neutral component

MEK

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ksr 
Kinase domain
sufficient binding region
aa 654-966
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as recombinant fusion proteins in a baculovirus and insect cell system.

cryoEM structure of ave-cnk-ksr-Dsor1 complex captured as a series of binary interactions that are not all direct. cryoEM indicates direct interaction between ave-cnk, ksr-Dsor1, cnk-ksr and cnk-Dsor1.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)