Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

Overall SAINT probability of interaction = 0.99.

Purified ave-cnk heterodimer was mixed with an equimolar ratio of ksr-Dsor1 heterodimer, which eluted as a higher order complex captured as series of binary interactions that do not necessarily indicate direct protein-protein contact. Mutations in cnk or ksr interfere with the higher order cnk-ave-ksr-Dsor1 complex.

Interaction in vitro; Dsor1 produced as a recombinant fusion protein in baculovirus and insect cell system; cnk produced as a recombinant fusion protein in bacterial cell system.

cryoEM structure of ave-cnk-ksr-Dsor1 complex captured as a series of binary interactions that are not all direct. cryoEM indicates direct interaction between ave-cnk, ksr-Dsor1, cnk-ksr and cnk-Dsor1.

Interaction in vitro; Dsor1 produced as a recombinant fusion protein in a baculovirus and insect system; cnk produced as a recombinant protein in bacterial system.

Co-expression of ave and ksr increase cnk-Dsor1 interaction with cnk aa 2-549 and 2-384 constructs, but not aa 2-209 which lacks the PDZ domain.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.