BamC, Bam-C
novel protein involved in oogenesis and spermatogenesis - functions as a translational repressor by interfering with translation initiation - controls the size and organization of the Drosophila hematopoietic niche through interactions with the Insulin-like growth factor pathway and Retinoblastoma-family protein - activates H3K36 trimethylation-mediated epigenetic regulation
Please see the JBrowse view of Dmel\bam for information on other features
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AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Gene model reviewed during 5.45
Gene model reviewed during 5.49
Gene model reviewed during 6.24
2.0 (northern blot)
There is only one protein coding transcript and one polypeptide associated with this gene
442 (aa); 49 (kD)
Interacts (via central region) with ubiquitin (PubMed:28484036). Interacts (via C-terminus) with otu (via OTU domain); the interaction enhances otu aggregation into amyloid-like structures and enhances its deubiquitinase activity (PubMed:28484036, PubMed:30879902). Together with otu interacts with CycA/cyclin-A (via C-terminus); the interaction stabilizes CycA by promoting and enhancing otu dependent deubiquitination of CycA (PubMed:28484036). Together with otu interacts with Traf6 (PubMed:30879902). Part of a complex composed of at least tut, bam and bgcn; complex formation does not require RNA (PubMed:25412508). Interacts (via C-terminus) with bgcn; the interaction is direct and is not disrupted by eIF4A (PubMed:19470484, PubMed:19556547, PubMed:23122292, PubMed:23526974, PubMed:25412508). Interacts with eIF4A (via multiple contacts); the interaction is direct and is not disrupted by bgcn (PubMed:19556547). Interacts (via N-terminus) with tut; the interaction is direct and mediates the interaction between tut and bgcn (PubMed:25412508). As part of the bam-bgcn-tut complex associates with twin; may recruit the CCR4-NOT1 deadenylation complex to mRNA 3'-UTRs to mediate post-transcriptional regulation of expression (PubMed:28190776). Part of a complex composed of at least mei-P26, bam, bgcn and Sxl; this complex is involved in translational repression of nanos mRNA (PubMed:23526974).
Ubiquitinated (C-terminal region).
May possess a C-terminal PEST sequence (rich in Pro, Glu, Ser and Thr) targeting it for degradation and rapid protein turnover.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\bam using the Feature Mapper tool.
The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).
Comment: not expressed at apical tip of testis
bam transcripts are expressed throughout oogenesis and in 0-2hr embryos but are absent by 4hr of development. They are also detected in dissected ovaries and testes. In ovaries, bam transcripts are expressed in cystoblasts and/or very early cystocytes. Later they are detected in stage 10 nurse cells, are transferred to the oocyte at the time of nurse cell breakdown, and persist in the mature egg and the early embryo..
JBrowse - Visual display of RNA-Seq signals
View Dmel\bam in JBrowse3-87
3-85.8
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
polyclonal
monoclonal
Forced premature expression of bam in early male germ cells (stem cells, gonialblasts and spermatogonia) leads to accumulation of male germ cells at the single-cell stage and then death of the early male germ cells.
The down-regulation of bam protein is essential for the initiation of cystocyte differentiation into functional egg chambers.
In a sample of 79 genes with multiple introns, 33 showed significant heterogeneity in G+C content among introns of the same gene and significant positive correspondence between the intron and the third codon position G+C content within genes. These results are consistent with selection adding against preferred codons at the start of genes.
Mosaic analysis demonstrates bam and bgcn act autonomously in the germline to restrict germ cell proliferation during spermatogenesis. Results suggest bam and bgcn regulate progression through the male germline stem cell lineage by cell-intrinsically restricting the proliferation of amplifying germ cells. bgcn function is not required for the proper expression of bam protein.
Mutations in bam disrupt cyst formation producing tumorous egg chambers.
bam is required to promote incomplete cytokinesis and activate fusome growth.
Mutants display germline hyperplastic phenotype.
bam is involved in the initiation of gametogenesis.
reference: Spradling and McKearin, personal communication.
bam is essential for male and female fertility but not for viability.
Named "bag of marbles" for cyst-like clusters of undifferentiated germ cells in mutant testes and ovaries resembling a bag of marbles.
