FB2026_03 , released September 17, 2026
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Term three hybrid ID (Ontology) MI:0588 (Molecular Interactions)
Definition Group of methods based on complementation assay where a third participant is shown to be necessary for the binding of a given bait prey pair. The molecule fused to the DNA binding domain is the bait, that fused to the transcriptional activator is the prey.[ PubMed:14755292 ]
Also Known As"3 hybrid" ; "3-hybrid" ; "tri hybrid" (for all, see Synonyms field below)
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 three hybrid (all annotations which use CV term, excluding "NOT" statements)
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  protein complementation assay
   |__transcriptional complementation assay
       |__three hybrid  54 rec.
           |__protein three hybrid 17 rec.
           |__rna three hybrid 21 rec.
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Is a transcriptional complementation assay
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Synonyms
  • "3 hybrid" EXACT PSI-MI-short
    "3-hybrid" EXACT PSI-MI-alternate
    "tri hybrid" EXACT PSI-MI-alternate
    "tri-hybrid assay" EXACT PSI-MI-alternate
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