FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
bam, nanos
FlyBase ID
FBig0000114900
Interaction Network
Interactions Browser links
bam network
nanos network
Reported Interactions
FBrf0209919-9.coIP.WB
Reference
Kim et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
bam 

Bam

bait

FLAG tag

nanos 

Nos

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Kim et al., 2010)
FBrf0247403-1.coIP.PCR
Reference
Malik et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
bam 

Bam

bait

FLAG tag

nanos 

Nos

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Malik et al., 2020)
FBrf0247403-9.coIP.PCR
Reference
Malik et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
bam 

Bam

bait

FLAG tag

nanos 

Nos

prey
nanos 
nanos 
nanos 
nanos 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
nanos 
3-prime UTR proximal
sufficient binding region
nt 21-100
nanos 
3-prime UTR miRNA binding sites
mutation disrupting interaction
nt 34-40,76-81

GGCAGCU to GGUAGUU and CAUUCC to CAUUUU

nanos 
3-prime UTR distal
sufficient binding region
nt 781-851
nanos 
3-prime UTR Sxl binding site
mutation disrupting interaction
nt 787-800

UUUUUUUAUUUUUU to UUUCUCUAUCUCUU

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Malik et al., 2020)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)