FlyBase Allele Report: Dmel\bam[ΔPEST]

General Information

Symbol

Dmel\bam^ΔPEST

Species

D. melanogaster

Name

FlyBase ID

FBal0243774

Feature type

allele

Associated gene

Dmel\bam

Associated Insertion(s)

Carried in Construct

P{bam.ΔPEST}

Key Links

Transgenic product class

frameshift_truncation

(Insco et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

frameshift_truncation

(Insco et al., 2009)

Mutagen

in vitro construct

(Insco et al., 2009)

Progenitor genotype

Carried in construct

P{bam.ΔPEST}

Cytology

Description

Fragment containing bam promoter and coding sequence, encoding a frameshift mutation in the coding region that changes the identity of 7aa and deletes 22 more subsequent amino acids (removing most of the PEST sequence and the rest of the C-terminus).

(Insco et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

bam

Encoded product / tool

bam

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell number

(Insco et al., 2009)

decreased cell number, with bam^Δ86

(Insco et al., 2009)

Phenotype Manifest In

spermatid

(Insco et al., 2009)

spermatocyte

(Insco et al., 2009)

spermatogonial cyst

(Insco et al., 2009)

Detailed Description

Statement

Reference

Mutant males carrying one copy of bam^ΔPEST in a wild-type background show premature spermatocyte differentiation of germline cysts at the 8-cell stage in 9-18% of cysts. 40% of cysts prematurely differentiate at the 8-cell stage in males carrying one copy of bam^ΔPEST in a bam^Δ86/+ background. Males carrying two copies of bam^ΔPEST in a wild-type background show premature differentiation of germline cysts at the 8-cell stage in 68% of cysts. In these cases, the 8-cell cysts have large cells with typical spermatocyte morphology. Post-meiotic cysts are observed with 32 instead of 64 elongating spermatids.

(Insco et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressor of

Statement

Reference

bam^ΔPEST is a suppressor | partially of male germline cyst phenotype of mei-P26^mfs1

(Insco et al., 2012)

bam^ΔPEST is a suppressor of nucleolus phenotype of mei-P26^mfs1

(Insco et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of bam^ΔPEST completely rescues the overproliferation defects seen in the testes of mei-P26^mfs1 males and 43% of the cysts in the spermatocyte region have 16 spermatocytes per cyst. The enlarged nucleolus phenotype seen in mei-P26^mfs1 testes is also suppressed. The spermatocyte and spermatid differentiation defects are not rescued: 30% of cysts from the spermatocyte region have differentiation defects in the rescued males.

(Insco et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

bam^ΔPEST

(Eikenes et al., 2013, Insco et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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