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General Information
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| Term |
iclip |
ID (Ontology) |
MI:2193 (Molecular Interactions) |
| Definition |
iCLIP allows for the stringent purification of UV cross-linked protein-RNA complexes, using immunoprecipitation followed by SDS-PAGE and membrane transfer. The radiolabelled protein-RNA complexes are then excised from the membrane, and treated with proteinase to release the RNA. This leaves one or two amino acids at the RNA cross-link site. The RNA is then reverse transcribed using barcoded primers. Because reverse transcription stops prematurely at the cross-link site, iCLIP allows RNA-protein interaction sites to be identified at high resolution.\nhttps://en.wikipedia.org/wiki/ICLIP[ PubMed:20601959 ] |
| Also Known As |
"iclip" ; "iCLIP" ; "individual-nucleotide resolution cross-linking and immunoprecipitation" |
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Links to External Ontologies
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Annotations
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Records annotated with this term OR any of its CHILD TERMS
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Records annotated with this exact term (annotations to child terms are NOT included)
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No relevant records available
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Full annotation statements including this term (annotations to child terms are NOT included), and relevant FlyBase records
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| Full annotation statements | Relevant FlyBase reports |
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| Interaction Groups |
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iclip (all annotations which use CV term, excluding "NOT" statements) | 43 |
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Relationships