FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Not1, bam
FlyBase ID
FBig0000116449
Interaction Network
Interactions Browser links
Not1 network
bam network
Reported Interactions
FBrf0238143-1.coIP.WB
Reference
Sgromo et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Not1 

NOT1

bait

GFP tag

bam 

Bam

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Sgromo et al., 2018)
FBrf0238143-6.PD.MW
Reference
Sgromo et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
bam 

Bam

bait

MBP tag

bam 
bam 
bam 
bam 
Not1 

NOT1

prey
Not1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
bam 
CBM domain
sufficient binding region
aa 13-36
bam 
CBM domain
necessary binding region
aa 13-36
bam 
CBM domain residue 17
mutation disrupting interaction
aa 17

L17E

bam 
CBM domain residue 24
mutation disrupting interaction
aa 24

M24E

Not1 
CN9BD domain
sufficient binding region
aa 1468-1719

coordinates relative to Not1-PC

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The Not1 CN9BD domain and Rcd-1 ARM domain were co-purified and used together as prey in the pull-down experiment. Mutations in the Rcd-1 ARM domain disrupted the bam-Not1 interaction.

(Sgromo et al., 2018)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; preys produced produced as a recombinant fusion proteins in bacterial system.

(Sgromo et al., 2018)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)