FlyBase Allele Report: Dmel\InR[339]

General Information

Symbol

Dmel\InR³³⁹

Species

D. melanogaster

Name

FlyBase ID

FBal0122080

Feature type

allele

Associated gene

Dmel\InR

Associated Insertion(s)

Carried in Construct

Also Known As

dinr³³⁹

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Cytology

Description

Amino acid replacement: G1551E.

(Brogiolo et al., 2001)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:21,575,219..21,575,219 [-]

Nucleotide change:

G21575219A

Amino acid change:

G1536D | InR-PA; G1536D | InR-PB; G1536D | InR-PC; G1536D | InR-PD

Reported amino acid change:

G1491D

Comment:

Mutation is in the cytoplasmic region.

Comment:

Site of nucleic acid difference in mutant inferred by FlyBase based on reported amino acid change.

(Brogiolo et al., 2001)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates germ cell cancer

modeled by bam^Δ86

(Zhao et al., 2018)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell number (with InR^E19), with Scer\GAL4^bab1-Gal4

(Yang et al., 2013)

decreased cell number | oogenesis (with InR^E19)

(Su et al., 2018)

decreased cell size | somatic clone

(Lee et al., 2009)

male sterile (with InR^E19)

(Ueishi et al., 2009)

viable | male (with InR^E19)

(Ueishi et al., 2009)

Phenotype Manifest In

egg chamber | germline clone

(LaFever and Drummond-Barbosa, 2005)

escort cell (with InR^E19)

(Su et al., 2018)

female germline stem cell (with InR^E19), with Scer\GAL4^bab1-Gal4

(Yang et al., 2013)

female germline stem cell | germline clone

(Tseng et al., 2014, LaFever and Drummond-Barbosa, 2005)

female germline stem cell | somatic clone

(Kao et al., 2015)

germarium cap cell (with InR^E19), with Scer\GAL4^bab1-Gal4

(Yang et al., 2013)

germline cyst | germline clone

(LaFever and Drummond-Barbosa, 2005)

male germline stem cell (with InR^E19)

(Ueishi et al., 2009)

polar primary spermatocyte (with InR^E19)

(Ueishi et al., 2009)

primary spermatocyte (with InR^E19)

(Ueishi et al., 2009)

rhabdomere | somatic clone

(Lee et al., 2009)

Detailed Description

Statement

Reference

InR^E19/InR³³⁹ transheterozygotes show a significantly decreased number of escort cells in the germarium, as compared to controls.

(Su et al., 2018)

InR³³⁹ mutant germline stem cells are lost more quickly than controls.

(Kao et al., 2015)

InR³³⁹ mutant female germline stem cells divide three times more slowly than wild type.

(Tseng et al., 2014)

InR³³⁹/InR^E19 mutant flies raised at 18[o]C contain fewer germline stem cells and cap cells than the respective controls. The number of GSCs and cap cells reduces further one week after being shifted to 29[o]C at eclosion.

(Yang et al., 2013)

Photoreceptor cell rhabdomeres are on average 30% the size of wild type in homozygous clones in the eye.

(Lee et al., 2009)

The average number of germline stem cells (GSCs) in the testis of InR^E19/InR³³⁹ males within 24 hours of eclosion is similar to that seen in wild-type males at this time. However, the mutant males show an increased rate of reduction in GSC number with age compared to wild-type males, such that 14 days after eclosion, while the wild-type males maintain 90% of the GSC numbers of newly eclosed males, the mutant males maintain only 70% of the average GSC number of newly eclosed males.

InR^E19/InR³³⁹ males that are within 24 hours of eclosion have a reduced number of stage S2b spermatocyte cysts in the testis compared to wild-type males. By 14 days after eclosion, stage S2b spermatocyte cysts are no longer detected in InR^E19/InR³³⁹ males.

The average diameter of stage S6 InR^E19/InR³³⁹ spermatocytes is smaller than that of control spermatocytes at this stage.

(Ueishi et al., 2009)

Homozygous female germline cysts have normal morphology and cell number, but 83% of these cysts are developmentally delayed, showing a markedly decreased size compared to neighbouring wild-type egg chambers. Mutant germline stem cells (GSCs) have a reduced division rate compared to wild-type GSCs. Egg chambers in mosaic ovarioles in which the entire germline is homozygous fail to progress through vitellogenesis and degenerate.

(LaFever and Drummond-Barbosa, 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

InR³³⁹ has decreased cell size | somatic clone phenotype, enhanceable by lqfR^Δ117

(Lee et al., 2009)

Suppressed by

Statement

Reference

InR^E19/InR³³⁹ has decreased cell number | oogenesis phenotype, suppressible by foxo²⁵/foxo²¹

(Su et al., 2018)

InR^E19/InR³³⁹ has decreased cell number phenotype, suppressible by N^ICN.UAS/Scer\GAL80^ts.αTub84B/Scer\GAL4^bab1-Gal4

(Yang et al., 2013)

InR^E19/InR³³⁹ has decreased cell number phenotype, suppressible by fng^GD2763/Scer\GAL80^ts.αTub84B/Scer\GAL4^bab1-Gal4

(Yang et al., 2013)

InR^E19/InR³³⁹ has decreased cell number phenotype, suppressible by N^ΔE.UAS/Scer\GAL80^ts.αTub84B/Scer\GAL4^bab1-Gal4

(Yang et al., 2013)

NOT suppressed by

Statement

Reference

InR^E19/InR³³⁹ has decreased cell number phenotype, non-suppressible by N^UAS.cMa/Scer\GAL80^ts.αTub84B/Scer\GAL4^bab1-Gal4

(Yang et al., 2013)

Enhancer of

Statement

Reference

InR³³⁹ is an enhancer of decreased cell size | somatic clone phenotype of lqfR^Δ117

(Lee et al., 2009)

InR³³⁹ is an enhancer of visible phenotype of Pi3K92E^{D954A.UAS.Tag:MYC}, Scer\GAL4^Bx-MS1096

(Coelho et al., 2005)

Phenotype Manifest In

Enhanced by

Statement

Reference

InR³³⁹ has rhabdomere | somatic clone phenotype, enhanceable by lqfR^Δ117

(Lee et al., 2009)

Suppressed by

Statement

Reference

InR^E19/InR³³⁹ has escort cell phenotype, suppressible by foxo²⁵/foxo²¹

(Su et al., 2018)

InR^E19/InR³³⁹ has female germline stem cell phenotype, suppressible by N^ΔE.UAS/Scer\GAL80^ts.αTub84B/Scer\GAL4^bab1-Gal4

(Yang et al., 2013)

InR^E19/InR³³⁹ has germarium cap cell phenotype, suppressible by N^ΔE.UAS/Scer\GAL80^ts.αTub84B/Scer\GAL4^bab1-Gal4

(Yang et al., 2013)

InR^E19/InR³³⁹ has female germline stem cell phenotype, suppressible by N^ICN.UAS/Scer\GAL80^ts.αTub84B/Scer\GAL4^bab1-Gal4

(Yang et al., 2013)

InR^E19/InR³³⁹ has germarium cap cell phenotype, suppressible by N^ICN.UAS/Scer\GAL80^ts.αTub84B/Scer\GAL4^bab1-Gal4

(Yang et al., 2013)

InR^E19/InR³³⁹ has female germline stem cell phenotype, suppressible by fng^GD2763/Scer\GAL80^ts.αTub84B/Scer\GAL4^bab1-Gal4

(Yang et al., 2013)

InR^E19/InR³³⁹ has germarium cap cell phenotype, suppressible by fng^GD2763/Scer\GAL80^ts.αTub84B/Scer\GAL4^bab1-Gal4

(Yang et al., 2013)

NOT suppressed by

Statement

Reference

InR^E19/InR³³⁹ has female germline stem cell phenotype, non-suppressible by N^UAS.cMa/Scer\GAL4^bab1-Gal4

(Yang et al., 2013)

InR^E19/InR³³⁹ has germarium cap cell phenotype, non-suppressible by N^UAS.cMa/Scer\GAL80^ts.αTub84B/Scer\GAL4^bab1-Gal4

(Yang et al., 2013)

Enhancer of

Statement

Reference

InR³³⁹ is an enhancer of rhabdomere | somatic clone phenotype of lqfR^Δ117

(Lee et al., 2009)

InR³³⁹ is an enhancer of wing phenotype of Pi3K92E^{D954A.UAS.Tag:MYC}, Scer\GAL4^Bx-MS1096

(Coelho et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

The decreased number of escort cells observed in the germarium of InR^E19/InR³³⁹ transheterozygotes is suppressed by additional foxo²¹/foxo²⁵ transheterozygosity.

(Su et al., 2018)

Expression of N^Scer\UAS.cMa under the control of Scer\GAL4^bab1-Gal4 (limited to the adult stages using Scer\GAL80^ts.αTub84B) does not suppress the germline stem cell and cap cell loss seen in InR³³⁹/InR^E19.

Expression of Dl::N^{ΔECN.Scer\UAS} under the control of Scer\GAL4^bab1-Gal4 (limited to the adult stages using Scer\GAL80^ts.αTub84B) significantly suppresses the germline stem cell and cap cell loss seen in InR³³⁹/InR^E19 mutant week old females.

Expression of N^ICN.Scer\UAS under the control of Scer\GAL4^bab1-Gal4 (limited to the adult stages using Scer\GAL80^ts.αTub84B) significantly suppresses the germline stem cell and cap cell loss seen in InR³³⁹/InR^E19 mutant week old females.

Expression of fng^GD2763 under the control of Scer\GAL4^bab1-Gal4 (limited to the adult stages using Scer\GAL80^ts.αTub84B) suppresses the germline stem cell and cap cell loss seen in InR³³⁹/InR^E19 mutant week old females.

(Yang et al., 2013)

Photoreceptor cell rhabdomeres are on average 23% the size of wild type in InR³³⁹ lqfR^Δ117 double mutant clones in the eye (significantly smaller than in either single mutant).

(Lee et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

98101

w^*; P{neoFRT}82B InR³³⁹/TM3, Sb¹ Ser¹

Notes on Origin

Discoverer

Comments

Alleles of InR form an allelic series based on phenotypic strength: InR³³⁹ > InR³¹ > InR²¹¹ > InR^E19 > InR³⁵³.

(Brogiolo et al., 2001)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

InR³³⁹

(Luo et al., 2020, Fernandes et al., 2017, Lee et al., 2009, Ueishi et al., 2009)

Inr³³⁹

(Su et al., 2018)

dinr³³⁹

(Kao et al., 2015, Tseng et al., 2014, Yang et al., 2013, LaFever and Drummond-Barbosa, 2005)

inr³³⁹

(Zhao et al., 2018)

Name Synonyms

Secondary FlyBase IDs

References (12)

Report Sections

Open Close