FlyBase Allele Report: Dmel\N[ΔE.UAS]

General Information

Symbol

Dmel\N^ΔE.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0048200

Feature type

allele

Associated gene

Dmel\N

Associated Insertion(s)

Carried in Construct

P{UAS-Delta.ΔE}

Also Known As

UAS-ΔN34a, UAS-Notch^ACT, UAS-N^intra, UAS-N^ACT, UAS-N^ΔE, N^act, UAS-N^ΔB2a2, UAS-NΔecd, N^IC, UAS-actN

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Fuerstenberg and Giniger, 1998, Baker and Schubiger, 1996, Doherty et al., 1996, Larkin et al., 1996)

Progenitor genotype

Carried in construct

P{UAS-Delta.ΔE}

Cytology

Description

UASt regulatory sequences drive expression of a constitutively activated, ligand-independent form of N. The signal sequence and signal cleavage site of Dl (amino acid residues 1-27) are fused to N sequence from amino acid 1742 to the end of the coding sequence. A frameshift mutation in the N coding sequence causes premature termination after amino acid residue 2235 with 4 residues (RPPT) appended to the C terminus.

(Fuerstenberg and Giniger, 1998)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Tagged with

Tag:SS(Delta)

signal sequence

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of central nervous system cancer

CEA

(Voutyraki et al., 2023, Voutyraki et al., 2022)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of central nervous system cancer

is exacerbated by InR^A1325D.UAS

(Voutyraki et al., 2023)

is ameliorated by InR^K1409A.UAS

(Voutyraki et al., 2023)

is ameliorated by Imp^HMS01168

(Voutyraki et al., 2023)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell death, with Scer\GAL4^eg-Mz360

(Lee et al., 2013, Lundell et al., 2003)

abnormal cell shape | somatic clone, with Scer\GAL4^e22c

(Boyle and Berg, 2009)

abnormal mitotic cell cycle, with Scer\GAL4^bbg-C96

(Johnston and Edgar, 1998)

abnormal neuroanatomy, with Scer\GAL4^eg-Mz360

(Lundell et al., 2003)

abnormal neuroanatomy, with Scer\GAL4^elav-C155

(Umesono et al., 2002)

abnormal neuroanatomy | larval stage | somatic clone, with Scer\GAL4^elav.PU

(Wu et al., 2017)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^insc-Mz1407, Scer\GAL80^ase.PZ

(Zhu et al., 2012)

decreased cell number | embryonic stage, with Scer\GAL4^elav.PLu

(Drake et al., 2025)

increased cell number | larval stage | somatic clone, with Scer\GAL4^elav.PU

(Wu et al., 2017)

increased size | adult stage, with Scer\GAL4^GMR.PU

(Nagel et al., 2021)

lethal, with Scer\GAL4^c768

(Wang et al., 2011)

lethal, with Scer\GAL4^C855a

(Wang et al., 2011)

lethal - all die before end of larval stage, with Scer\GAL4^bap.3

(Stute et al., 2004)

lethal - all die before end of pupal stage, with Scer\GAL4^ptc-559.1

(Doherty et al., 1996)

neoplasia | larval stage | somatic clone, with Scer\GAL4^elav.PU

(Wu et al., 2017)

some die during larval stage, with Scer\GAL4^bap.3

(Stute et al., 2004)

some die during pupal stage, with Scer\GAL4^ptc-559.1

(Doherty et al., 1996)

visible, with Scer\GAL4^Act5C.PI

(Rauskolb and Irvine, 1999)

visible, with Scer\GAL4^ptc-559.1

(Rauskolb and Irvine, 1999)

visible | adult stage, with Scer\GAL4^GMR.PU

(Nagel et al., 2021)

Phenotype Manifest In

abdominal dorsal bipolar neuron dbp, with Scer\GAL4^elav-C155

(Umesono et al., 2002)

border follicle cell | somatic clone | increased number, with Scer\GAL4^Act5C.PI

(Grammont and Irvine, 2002)

chordotonal organ precursor cell & ventral thoracic disc, with Scer\GAL4^sca-109-68

(zur Lage and Jarman, 1999)

embryonic/larval optic lobe | temperature conditional, with Scer\GAL4^c768, Scer\GAL80^ts.αTub84B

(Wang et al., 2011)

embryonic/larval optic lobe | temperature conditional, with Scer\GAL4^C855a, Scer\GAL80^ts.αTub84B

(Wang et al., 2011)

embryonic/larval trachea & tracheal tip cell, with Scer\GAL4^btl.PS

(Ikeya and Hayashi, 1999)

embryonic/larval tracheal dorsal trunk, with Scer\GAL4^btl.PS

(Ikeya and Hayashi, 1999)

embryonic/larval tracheal section, with Scer\GAL4^btl.PS

(Ikeya and Hayashi, 1999)

embryonic abdominal segment, with Scer\GAL4^elav.PLu

(Drake et al., 2025)

embryonic GW neuron | ectopic, with Scer\GAL4^eg-Mz360

(Lundell et al., 2003)

embryonic hindgut, with Scer\GAL4^455.2

(Iwaki and Lengyel, 2002)

embryonic hindgut, with Scer\GAL4^byn-Gal4

(Iwaki and Lengyel, 2002, Takashima et al., 2002)

embryonic large intestine, with Scer\GAL4^byn-Gal4

(Takashima et al., 2002)

embryonic segment, with Scer\GAL4^69B

(Fuerstenberg and Giniger, 1998)

epidermis, with Scer\GAL4^twi.PBa

(Baker and Schubiger, 1996)

EW1 neuron, with Scer\GAL4^eg-Mz360

(Lundell et al., 2003)

EW2 neuron, with Scer\GAL4^eg-Mz360

(Lundell et al., 2003)

EW3 neuron, with Scer\GAL4^eg-Mz360

(Lundell et al., 2003)

EW3 neuron | larval stage, with Scer\GAL4^eg-Mz360

(Lee et al., 2013)

external sensory organ precursor cell, with Scer\GAL4^sca-109-68

(zur Lage and Jarman, 1999)

exuvial fluid | pupal stage, with Scer\GAL4¹¹⁵¹

(Chu and Hayashi, 2021)

eye, with Scer\GAL4^GMR.PU

(Nagel et al., 2021)

eye, with Scer\GAL4^sca-109-68

(Li et al., 2003)

eye disc | dorsal, with Scer\GAL4^Act5C.PI

(Papayannopoulos et al., 1998)

intermediate neuronal progenitor | increased number | third instar larval stage, with Scer\GAL4^insc-Mz1407, Scer\GAL80^ase.PZ

(Zhu et al., 2012)

larval brain | larval stage | somatic clone, with Scer\GAL4^elav.PU

(Wu et al., 2017)

larval dorsal multidendritic neuron, with Scer\GAL4^elav-C155

(Umesono et al., 2002)

larval glial cell | ectopic, with Scer\GAL4^elav-C155

(Umesono et al., 2002)

larval outer optic anlage | somatic clone, with Scer\GAL4^Act.PU

(Wang et al., 2011)

larval somatic muscle cell, with Scer\GAL4^112A

(Fuerstenberg and Giniger, 1998)

larval somatic muscle cell, with Scer\GAL4^twi.PBa

(Fuerstenberg and Giniger, 1998)

leg, with Scer\GAL4^Act5C.PI

(Rauskolb and Irvine, 1999)

leg, with Scer\GAL4^ptc-559.1

(Rauskolb and Irvine, 1999)

leg disc, with Scer\GAL4^ptc-559.1

(Hao et al., 2003)

leg joint, with Scer\GAL4^ptc-559.1

(Rauskolb and Irvine, 1999)

male genitalia, with Scer\GAL4^Abd-B-LDN

(Lee et al., 2011)

medulla anlage | temperature conditional, with Scer\GAL4^c768, Scer\GAL80^ts.αTub84B

(Wang et al., 2011)

medulla anlage | temperature conditional, with Scer\GAL4^C855a, Scer\GAL80^ts.αTub84B

(Wang et al., 2011)

microtubule, with Scer\GAL4^109(3)9

(Larkin et al., 1996)

muscle founder cell, with Scer\GAL4^twi.PBa

(Baker and Schubiger, 1996)

neuron, with Scer\GAL4^112A

(Larkin et al., 1996)

neuron, with Scer\GAL4^elav.PLu

(Drake et al., 2025)

neuron, with Scer\GAL4^insc-Mz1407

(Guo et al., 1996)

oocyte | oogenesis stage S10B | somatic clone, with Scer\GAL4^e22c

(Boyle and Berg, 2009)

photoreceptor cell R1, with Scer\GAL4^hs.2sev

(Nagaraj and Banerjee, 2009)

photoreceptor cell R6, with Scer\GAL4^hs.2sev

(Nagaraj and Banerjee, 2009)

photoreceptor cell R8, with Scer\GAL4^sca-109-68

(Li et al., 2003)

polar follicle cell, with Scer\GAL4^hs.PB

(Larkin et al., 1996)

polar follicle cell | increased number, with Scer\GAL4^unspecified

(Grammont and Irvine, 2001)

polar follicle cell | somatic clone | increased number, with Scer\GAL4^Act5C.PI

(Grammont and Irvine, 2002)

presumptive embryonic/larval central nervous system, with Scer\GAL4^69B

(Fuerstenberg and Giniger, 1998)

presumptive embryonic/larval nervous system, with Scer\GAL4^112A

(Fuerstenberg and Giniger, 1998)

presumptive embryonic/larval nervous system, with Scer\GAL4^da.G32

(Giebel et al., 1997)

prothoracic metatarsus, with Scer\GAL4^Act5C.PI

(Rauskolb and Irvine, 1999)

prothoracic tarsal segment 3, with Scer\GAL4^Act5C.PI

(Rauskolb and Irvine, 1999)

sex comb, with Scer\GAL4^Act5C.PI

(Rauskolb and Irvine, 1999)

stalk follicle cell, with Scer\GAL4^hs.PB

(Larkin et al., 1996)

trichogen cell, with Scer\GAL4^sca-109-68

(Guo et al., 1996)

type II neuroblast | increased number | third instar larval stage, with Scer\GAL4^insc-Mz1407, Scer\GAL80^ase.PZ

(Zhu et al., 2012)

type II neuroblast | larval stage | somatic clone | increased number, with Scer\GAL4^elav.PU

(Wu et al., 2017)

unguis, with Scer\GAL4^ptc-559.1

(Rauskolb and Irvine, 1999)

wing disc, with Scer\GAL4^Act5C.PI

(Nakagoshi et al., 2002)

wing disc, with Scer\GAL4^bbg-C96

(Johnston and Edgar, 1998)

wing disc | somatic clone | ventral, with Scer\GAL4^Act5C.PI

(Rauskolb et al., 1999)

wing disc dorsal compartment, with Scer\GAL4^ptc-559.1

(Doherty et al., 1996)

wing disc ventral compartment, with Scer\GAL4^ptc-559.1

(Doherty et al., 1996)

Detailed Description

Statement

Reference

The expression of Dl::N^ΔECN.UAS under the control of Scer\GAL4¹¹⁵¹ results in the exuvial space failing to expand.

(Chu and Hayashi, 2021)

The expression of Dl::N^ΔECN.UAS under the control of Scer\GAL4^GMR.PU induces overgrowths in the eye.

(Nagel et al., 2021)

Larval central brain clones expressing Dl::N^{ΔECN.Scer\UAS} under the control of Scer\GAL4^elav.PU exhibit ectopic formation and increased numbers of type II neuroblasts, as well as tumor-like brain growth, as compared to controls.

(Wu et al., 2017)

Expression of Dl::N^{ΔECN.Scer\UAS} under the control of Scer\GAL4^Crz.PC does not trigger precocious death of vCrz neurons in larvae or during metamorphosis.

Expression of Dl::N^{ΔECN.Scer\UAS} under the control of Scer\GAL4^eg-Mz360 triggers precocious death of vCrz neurons in larvae.

(Lee et al., 2013)

Expression of Dl::N^{ΔECN.Scer\UAS} under the control of both Scer\GAL4^insc-Mz1407 and Scer\GAL80^ase.PZ results in an increase in the number of type II neuroblasts and numerous small non-ase-expressing immature intermediate neural progenitors (INPs) in third larval instar brains.

(Zhu et al., 2012)

100% of males expressing Dl::N^{ΔECN.Scer\UAS} under the control of Scer\GAL4^Abd-B-LDN show incomplete genitalia rotation.

(Lee et al., 2011)

Expression of Dl::N^{ΔECN.Scer\UAS} under the control of either Scer\GAL4^C855a or Scer\GAL4^c768 during the third larval instar stage (using the temperature sensitive Scer\GAL80^ts.αTub84B allele to temporally limit expression) results in an expansion of neuroepithelial cells in the optic lobe while medulla neurogenesis is suppressed compared to wild type.

Expression of Dl::N^{ΔECN.Scer\UAS} under the control of Scer\GAL4^Act.PU in clones in the outer proliferation center of the larval optic lobe results in ectopic neuroepithelial cells which form rosettes separated from the neuroepithelium.

(Wang et al., 2011)

Follicular cells expressing Dl::N^{DeltaECN.Scer\UAS} under the control of Scer\GAL4^e22c have normal cell shape at S9, but constricted cell apices at S10B.

(Boyle and Berg, 2009)

Expression of Dl::N^{ΔECN.Scer\UAS} in cells behind the morphogenetic furrow in the developing eye disc, under the control of Scer\GAL4^hs.2sev causes the loss of R1 and R6 photoreceptor cells.

(Nagaraj and Banerjee, 2009)

Cells in the pretarsal epithelium at the tip of the late third instar leg disc that are expressing Dl::N^{ΔECN.Scer\UAS} under the control of Scer\GAL4^ptc-559.1 appear to invaginate, resulting in an indentation in the epithelium. This is in contrast to control discs, where the pretarsal epithelium forms a flat sheet at the same stage.

(Hao et al., 2003)

When Dl::N^{ΔECN.Scer\UAS} is driven by Scer\GAL4^sca-109-68 R8 photoreceptor cells are often missing. Adult eyes are small and rough.

(Li et al., 2003)

Expression of Dl::N^{ΔECN.Scer\UAS} under the control of Scer\GAL4^eg-Mz360 disrupts the differentiation of the NB7-3 progeny; EW1, EW2 and EW3 are undetectable. Most EW1 cells appear to have been converted to a GW cell fate (as assayed by molecular marker expression). Novel apoptosis within the NB7-3 lineage is seen in the mutant embryos. Some of these animals eclose as adults, but die within several days.

(Lundell et al., 2003)

Follicles containing clones expressing Dl::N^{ΔECN.Scer\UAS} under the control of Scer\GAL4^Act5C.PI can show an increase in the number of polar cells at their normal location at the anterior and posterior ends of the follicle. In each case, mosaic follicles with an increased number of polar cells show an increase in the number of outer border cells. The additional polar cells are surrounded by outer border follicle cells and they migrate normally through the nurse cells. The number of outer border cells that are specified and migrate with each cluster is proportional to the number of additional polar cells.

(Grammont and Irvine, 2002)

Expression of Dl::N^{ΔECN.Scer\UAS} under the control of Scer\GAL4^byn-Gal4 results in embryos with hindguts that are dramatically shorter and wider than wild type. The lumen of the hindgut is wider than normal and there are more cells than normal in the circumference of the hindgut. Expression of Dl::N^{ΔECN.Scer\UAS} under the control of Scer\GAL4^455.2 does not affect hindgut elongation, but ectopic hindgut boundary cells are seen.

(Iwaki and Lengyel, 2002)

Expression of Dl::N^{ΔECN.Scer\UAS} under the control of Scer\GAL4^Act5C.PI in clones in the wing disc can cause prominent outgrowth of the disc.

(Nakagoshi et al., 2002)

Expression of Dl::N^{ΔECN.Scer\UAS} under the control of Scer\GAL4^byn-Gal4 results in the dorsal portion of the large intestine developing into border cells, resulting in a hump-like bulge.

(Takashima et al., 2002)

Expression of Dl::N^{ΔECN.Scer\UAS} under the control of Scer\GAL4^elav-C155 results in the dorsal bipolar dendritic (dbd) neuron being replaced with an extra repo-positive cell which resembles a glial cell in 83% of hemisegments. The dorsal dendritic arbor (dda) neurons are absent in these embryos.

(Umesono et al., 2002)

When expression is driven in clones by Scer\GAL4^unspecified extra polar cells develop at the termini of the egg chamber, though not in the main body follicle cells.

(Grammont and Irvine, 2001)

When Dl::N^{ΔECN.Scer\UAS} is expressed in embryos under the control of Scer\GAL4^btl.PS, all esg-expressing cells disappear from the tip of the fusion branches in the tracheal system and there is no sign of contact between fusion branches. In addition, anterior migration of the dorsal trunk is inhibited and it becomes short and thick. The total number of tracheal cells per metamere is not significantly different from control embryos.

(Ikeya and Hayashi, 1999)

Ectopic joint structures are produced perpendicular to the normal axis in the legs of flies expressing Dl::N^{ΔECN.Scer\UAS} under the control of Scer\GAL4^ptc-559.1. Truncation of the leg and disruption of claw development is also seen. Clones in tarsal segment 1 of the prothoracic leg expressing Dl::N^{ΔECN.Scer\UAS} under the control of Scer\GAL4^Act5C.PI show duplicated sex combs. Clones in the leg expressing Dl::N^{ΔECN.Scer\UAS} under the control of Scer\GAL4^Act5C.PI result in outgrowth of leg tissue. The outgrowth is composed of a mixture of wild-type cells and cells expressing Dl::N^{ΔECN.Scer\UAS} under the control of Scer\GAL4^Act5C.PI. Sex combs, which normally form on tarsal segment 1, are also sometimes seen on tarsal segment 3 of the prothoracic leg.

(Rauskolb and Irvine, 1999)

Flip-out somatic clones in the wing disc, in which there is clonal ectopic expression of Dl::N^{ΔECN.Scer\UAS}, can respect the dorsal-ventral border, and those clones produced early enough to be of mixed dorsal and ventral identity can extend across the border without disrupting compartmentalization.

(Rauskolb et al., 1999)

Mature sensory organ precursors (SOPs) of the femoral chordotonal sense organ are missing in leg discs in which Dl::N^{ΔECN.Scer\UAS} is expressed under the control of Scer\GAL4^sca-109-68. Formation of bristle SOPs is also strongly suppressed in these animals.

(zur Lage and Jarman, 1999)

Embryos expressing Dl::N^{ΔECN.Scer\UAS} in the mesoderm under the control of Scer\GAL4^twi.PBa show disruption of muscle development, although muscle progenitor cells are initially specified normally. Expression of Dl::N^{ΔECN.Scer\UAS} in the ectoderm under the control of Scer\GAL4^112A does not affect overall ectodermal morphology, but causes a striking disruption of muscle patterning; many unfused myoblasts are present and muscle fibres are often inappropriately positioned or formed (thinner than normal). Most hemisegments show some duplication or loss of muscles and some fused syncytia are rounded and unattached to the epidermis. There is some suppression of neurogenesis in these embryos. Expression of Dl::N^{ΔECN.Scer\UAS} in the ectoderm under the control of Scer\GAL4^69B results in some disruption of the central nervous system and some loss of uniformity in segmentation.

(Fuerstenberg and Giniger, 1998)

ZNC is still present in the developing wing of animals expressing Dl::N^{ΔECN.Scer\UAS} under the control of Scer\GAL4^C96, though the cells arrest in G1.

(Johnston and Edgar, 1998)

The dorsal half of the eye disc is enlarged in larvae expressing Dl::N^{ΔECN.Scer\UAS} under the control of Scer\GAL4^Act5C.PI.

(Papayannopoulos et al., 1998)

Scer\GAL4^da.G32-mediated expression generates aneural embryos.

(Giebel et al., 1997)

Presence of Scer\GAL4^twi.PBa causes abnormal epidermal development and a hypertrophy of muscle founder cells.

(Baker and Schubiger, 1996)

Shows an antineurogenic activity when expressed from Scer\GAL4^h-1J3. When driven by Scer\GAL4^ptc-559.1 at 16^oC animals die as early pupae, but third instar wing discs display extreme outgrowths in dorsal and ventral compartments.

(Doherty et al., 1996)

Scer\GAL4¹⁴⁰⁷ induced expression causes transformation of neurons to sheath cells. Scer\GAL4^sca-109-68 induced expression causes 90% of the bristles in the notum to form double sockets, or less frequently triple or four sockets.

(Guo et al., 1996)

Scer\GAL4^112A induced expression causes a neurogenic phenotype. Scer\GAL4^hs.PB induced expression causes long stalk formation in the germarium, a fully formed stalk is not affected. Cellular morphology of the stalk resembles wild type stalks. The severity of the stalk phenotype correlates with the length of the precursor cell stage, culminating in a complete block at the precursor stage. Scer\GAL4^hs.PB induced expression also causes loss of anterior and posterior polar cells of the egg chambers, causing a defect in oocyte anterior-posterior axis. Egg chambers do not develop further and become necrotic. Scer\GAL4^109(3)9 induced expression causes premature ooplasmic streaming in stage 8 oocytes.

(Larkin et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

N^ΔE.UAS, Scer\GAL4^elav.PU has abnormal neuroanatomy | somatic clone | larval stage phenotype, suppressible | somatic clone by Dcr-1^Q1948X

(Wu et al., 2017)

N^ΔE.UAS, Scer\GAL4^elav.PU has neoplasia | somatic clone | larval stage phenotype, suppressible | somatic clone by Dcr-1^Q1948X

(Wu et al., 2017)

N^ΔE.UAS, Scer\GAL4^elav.PU has increased cell number | somatic clone | larval stage phenotype, suppressible | somatic clone by Dcr-1^Q1948X

(Wu et al., 2017)

N^ΔE.UAS, Scer\GAL4^elav.PU has abnormal neuroanatomy | somatic clone | larval stage phenotype, suppressible | somatic clone by Df(3L)mir-ban^Δ1

(Wu et al., 2017)

N^ΔE.UAS, Scer\GAL4^elav.PU has neoplasia | somatic clone | larval stage phenotype, suppressible | somatic clone by Df(3L)mir-ban^Δ1

(Wu et al., 2017)

N^ΔE.UAS, Scer\GAL4^elav.PU has increased cell number | somatic clone | larval stage phenotype, suppressible | somatic clone by Df(3L)mir-ban^Δ1

(Wu et al., 2017)

Suppressor of

Statement

Reference

N^ΔE.UAS, Scer\GAL80^ts.αTub84B, Scer\GAL4^bab1-Gal4 is a suppressor of decreased cell number phenotype of InR^E19/InR³³⁹

(Yang et al., 2013)

NOT Suppressor of

Statement

Reference

N^ΔE.UAS, Scer\GAL4^hh.PU is a non-suppressor of increased cell death | larval stage phenotype of Scer\GAL4^hh.PU, Spyo\Cas9^UAS.P2, wg^{UAS.wg+intergenic-1.pCFD6}

(Ewen-Campen and Perrimon, 2024)

Other

Statement

Reference

Mnr^EP555, N^ΔE.UAS, Scer\GAL4^GMR.PU has lethal - all die before end of pupal stage phenotype

(Nagel et al., 2021)

Phenotype Manifest In

Suppressed by

Statement

Reference

N^ΔE.UAS, Scer\GAL4^elav.PU has type II neuroblast | increased number | somatic clone | larval stage phenotype, suppressible | somatic clone by Dcr-1^Q1948X

(Wu et al., 2017)

N^ΔE.UAS, Scer\GAL4^elav.PU has larval brain | somatic clone | larval stage phenotype, suppressible | somatic clone by Dcr-1^Q1948X

(Wu et al., 2017)

N^ΔE.UAS, Scer\GAL4^elav.PU has type II neuroblast | increased number | somatic clone | larval stage phenotype, suppressible | somatic clone by Df(3L)mir-ban^Δ1

(Wu et al., 2017)

N^ΔE.UAS, Scer\GAL4^elav.PU has larval brain | somatic clone | larval stage phenotype, suppressible | somatic clone by Df(3L)mir-ban^Δ1

(Wu et al., 2017)

NOT suppressed by

Statement

Reference

N^ΔE.UAS, Scer\GAL4^da.G32 has presumptive embryonic/larval nervous system phenotype, non-suppressible by l(1)sc^UAS.cHa/da^UAS.cGa, Scer\GAL4^da.G32

(Giebel et al., 1997)

Suppressor of

Statement

Reference

Scer\GAL80^ts.αTub84B, N^ΔE.UAS, Scer\GAL4^bab1-Gal4 is a suppressor of germarium cap cell | progressive | heat sensitive phenotype of Scer\GAL4^bab1-Gal4, Scer\GAL80^ts.αTub84B, enok^GD4037

(Xin et al., 2013)

Scer\GAL80^ts.αTub84B, N^ΔE.UAS, Scer\GAL4^bab1-Gal4 is a suppressor | partially of female germline stem cell | progressive | heat sensitive phenotype of Scer\GAL4^bab1-Gal4, Scer\GAL80^ts.αTub84B, enok^GD4037

(Xin et al., 2013)

N^ΔE.UAS, Scer\GAL80^ts.αTub84B, Scer\GAL4^bab1-Gal4 is a suppressor of female germline stem cell phenotype of InR^E19/InR³³⁹

(Yang et al., 2013)

N^ΔE.UAS, Scer\GAL80^ts.αTub84B, Scer\GAL4^bab1-Gal4 is a suppressor of germarium cap cell phenotype of InR^E19/InR³³⁹

(Yang et al., 2013)

N^ΔE.UAS/Scer\GAL4^hs.2sev is a suppressor of photoreceptor cell R1 phenotype of phyl^hs.sev

(Nagaraj and Banerjee, 2009)

N^ΔE.UAS/Scer\GAL4^hs.2sev is a suppressor of photoreceptor cell R6 phenotype of phyl^hs.sev

(Nagaraj and Banerjee, 2009)

N^ΔE.UAS, Scer\GAL4^ey.PH is a suppressor of eye disc phenotype of Scer\GAL4^ey.PH, pnr^D4.UAS

(Maurel-Zaffran and Treisman, 2000)

N^ΔE.UAS, Scer\GAL4^ey.PH is a suppressor of photoreceptor neuron phenotype of Scer\GAL4^ey.PH, pnr^D4.UAS

(Maurel-Zaffran and Treisman, 2000)

NOT Suppressor of

Statement

Reference

N^ΔE.UAS, Scer\GAL4^hh.PU is a non-suppressor of wing disc posterior compartment | larval stage phenotype of Scer\GAL4^hh.PU, Spyo\Cas9^UAS.P2, wg^{UAS.wg+intergenic-1.pCFD6}

(Ewen-Campen and Perrimon, 2024)

N^ΔE.UAS/Scer\GAL4^ey.PH is a non-suppressor of eye disc phenotype of kto^T631

(Treisman, 2001)

N^ΔE.UAS/Scer\GAL4^ey.PH is a non-suppressor of photoreceptor neuron phenotype of kto^T631

(Treisman, 2001)

N^ΔE.UAS/Scer\GAL4^ey.PH is a non-suppressor of eye disc phenotype of skd^T13

(Treisman, 2001)

N^ΔE.UAS/Scer\GAL4^ey.PH is a non-suppressor of photoreceptor neuron phenotype of skd^T13

(Treisman, 2001)

N^ΔE.UAS/Scer\GAL4^ey.PH is a non-suppressor of eye disc phenotype of kto^T241

(Treisman, 2001)

N^ΔE.UAS/Scer\GAL4^ey.PH is a non-suppressor of photoreceptor neuron phenotype of kto^T241

(Treisman, 2001)

N^ΔE.UAS/Scer\GAL4^ey.PH is a non-suppressor of eye disc phenotype of skd^T413

(Treisman, 2001)

N^ΔE.UAS/Scer\GAL4^ey.PH is a non-suppressor of photoreceptor neuron phenotype of skd^T413

(Treisman, 2001)

N^ΔE.UAS/Scer\GAL4^ey.PH is a non-suppressor of eye disc phenotype of kto^T555

(Treisman, 2001)

N^ΔE.UAS/Scer\GAL4^ey.PH is a non-suppressor of photoreceptor neuron phenotype of kto^T555

(Treisman, 2001)

N^ΔE.UAS/Scer\GAL4^ey.PH is a non-suppressor of eye disc phenotype of skd^T606

(Treisman, 2001)

N^ΔE.UAS/Scer\GAL4^ey.PH is a non-suppressor of photoreceptor neuron phenotype of skd^T606

(Treisman, 2001)

Other

Statement

Reference

Delta^RevF10, N^ΔE.UAS, Scer\GAL4^byn-Gal4 has embryonic hindgut phenotype

(Takashima et al., 2002)

Delta^RevF10, N^ΔE.UAS, Scer\GAL4^byn-Gal4 has embryonic large intestine phenotype

(Takashima et al., 2002)

N^ΔE.UAS, Scer\GAL4^byn-Gal4, en^UAS.cTa has embryonic hindgut phenotype

(Takashima et al., 2002)

N^ΔE.UAS, Scer\GAL4^byn-Gal4, en^UAS.cTa has embryonic large intestine phenotype

(Takashima et al., 2002)

N^ΔE.UAS, Scer\GAL4^elav-C155, nub^UAS.cNa has larval glial cell | ectopic phenotype

(Umesono et al., 2002)

N^ΔE.UAS, Scer\GAL4^ey.PH, wg^UAS.cAa has eye disc phenotype

(Maurel-Zaffran and Treisman, 2000)

N^ΔE.UAS, Scer\GAL4^ey.PH, wg^UAS.cAa has photoreceptor neuron phenotype

(Maurel-Zaffran and Treisman, 2000)

N^ΔE.UAS, Scer\GAL4^Act5C.PI, fng^UAS.cKa has wing disc | ventral | somatic clone phenotype

(Rauskolb et al., 1999)

N^ΔE.UAS, Scer\GAL4^bbg-C96, wg^unspecified has wing disc phenotype

(Johnston and Edgar, 1998)

Additional Comments

Genetic Interactions

Statement

Reference

The co-expression of Dl::N^ΔECN.UAS and pnut^GD1512 from the second instar larval stage under the combined control of Scer\GAL4^ap-md544 and Gal80[ts] does not leads to the formation of tumors in the third instar larval wing disc.

(Gerlach et al., 2018)

Presence of the Df(3L)ban^Δ1 or Df(3L)ban^Δ1 mutations leads to full suppression of the ectopic neuroblast formation, increased neuroblast numbers and tumorigenesis seen in clones expressing Dl::N^{ΔECN.Scer\UAS} under the control of Scer\GAL4^elav.PU.

(Wu et al., 2017)

The germarium cap cell loss resulting from the expression of enok^GD4037 can be fully rescued by forced expression of Dl::N^{ΔECN.Scer\UAS} under the control of Scer\GAL4^bab1-Gal4 and Scer\GAL80^ts.αTub84B.

The germline stem cell loss resulting from the expression of enok^GD4037 can be partially rescued by forced expression of Dl::N^{ΔECN.Scer\UAS} under the control of Scer\GAL4^bab1-Gal4 and Scer\GAL80^ts.αTub84B.

(Xin et al., 2013)

Expression of Dl::N^{ΔECN.Scer\UAS} under the control of Scer\GAL4^bab1-Gal4 (limited to the adult stages using Scer\GAL80^ts.αTub84B) significantly suppresses the germline stem cell and cap cell loss seen in InR³³⁹/InR^E19 mutant week old females.

(Yang et al., 2013)

The presence of Ras85D^V12.sev in cells expressing Dl::N^{ΔECN.Scer\UAS} under the control of Scer\GAL4^hs.2sev suppresses the loss of R1 and R6 photoreceptor cells.

(Nagaraj and Banerjee, 2009)

When Dl::N^{ΔECN.Scer\UAS} and en^Scer\UAS.cTa are co-expressed under the control of Scer\GAL4^byn-Gal4, the entire large intestine differentiates into boundary cells. Expression of Dl::N^{ΔECN.Scer\UAS} under the control of Scer\GAL4^byn-Gal4 in a Dl^RevF10 mutant background results in boundary cell differentiation throughout the entire large intestine.

(Takashima et al., 2002)

Embryos coexpressing Dl::N^{ΔECN.Scer\UAS} and nub^Scer\UAS.cNa under the control of Scer\GAL4^elav-C155 have a few extra repo-positive glial cells dorsally to the dorsal dendritic arbor (dda) lineage.

(Umesono et al., 2002)

Flies expressing both pnr^D4.Scer\UAS and Dl::N^{ΔECN.Scer\UAS} under the control of Scer\GAL4^ey.PH show restored photoreceptor differentiation in the eye disc. Flies expressing both wg^Scer\UAS.cAa and Dl::N^{ΔECN.Scer\UAS} under the control of Scer\GAL4^ey.PH show photoreceptor differentiation initiating all around the margins in the eye disc.

(Maurel-Zaffran and Treisman, 2000)

Flip-out somatic clones in the wing disc, in which there is clonal ectopic expression of both Dl::N^{ΔECN.Scer\UAS} and fng^Scer\UAS.cKa, show a slight but consistent extension into the dorsal compartment.

(Rauskolb et al., 1999)

Leg discs in which both rho^Scer\UAS.cGa and Dl::N^{ΔECN.Scer\UAS} are expressed under the control of Scer\GAL4^sca-109-68 have a phenotype resembling that of rho^Scer\UAS.cGa expressed under the control of Scer\GAL4^sca-109-68 rather than that of Dl::N^{ΔECN.Scer\UAS} expressed under the control of Scer\GAL4^sca-109-68.

(zur Lage and Jarman, 1999)

When discs expressing Dl::N^{ΔECN.Scer\UAS} under the control of Scer\GAL4^C96 are also mutant for wg, no ZNC forms at all.

(Johnston and Edgar, 1998)

Simultaneous expression of da^Scer\UAS.cGa and l(1)sc^Scer\UAS.cHa does not rescue the aneural phenotype caused by Scer\GAL4^da.G32-mediated expression of Dl::N^{ΔECN.Scer\UAS}.

(Giebel et al., 1997)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (3)

Bloomington

5830

y¹ w^*; P{UAS-Delta.ΔE}B2a2

26674

y¹ w^*; P{UAS-Delta.ΔE}B2a3

Kyoto

108452

y¹ w^*; P{UAS-Delta.ΔE}B2a2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (13)

Reported As

Symbol Synonym

Delta::N^ΔECN.UAS

Dl::N^UAS.cDa

Dl::N^{ΔECN.Scer\UAS}

Dl::N^ΔECN.UAS

N^IC

(Piggott et al., 2019, Zhu et al., 2012)

N^act

(Wu et al., 2017, Vachias et al., 2010, Sakata et al., 2004)

N^int.D.UAS

N^int.UAS

N^ΔE.UAS

N^ΔE

(Johnston and Edgar, 1998)

N^ΔEN.UAS

caN-mild

(Larkin et al., 1996)

Name Synonyms

constitutively active N-mild

(Larkin et al., 1996)

Secondary FlyBase IDs

FBal0048201
FBal0058513
FBal0060602

References (68)

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