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General Information
Symbol
P{UAS-Dl::N.ΔECN}
FlyBase ID
FBtp0001306
Feature type
transgenic_transposable_element
Component Allele(s)
Also Known As
UAS-ΔN34a, UAS-NotchACT, UAS-Nintra, UAS-NACT, UAS-NΔE, UAS-NΔB2a2, UAS-actN, UAS-NCA, UAS-NICD
Size
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Location-dependent role
Description
CV term
Qualifiers and info
Reference
Construct components
Product class / Tool use(s)
Marker(s)
Component allele Dl::NΔECN.UAS
Product class / Tool use(s)
Description

UASt regulatory sequences drive expression of a constitutively activated, ligand-independent form of N. The signal sequence and signal cleavage site of Dl (amino acid residues 1-27) are fused to N sequence from amino acid 1742 to the end of the coding sequence. A frameshift mutation in the N coding sequence causes premature termination after amino acid residue 2235 with 4 residues (RPPT) appended to the C terminus.

Component allele w+mC
Product class / Tool use(s)
Regulatory region(s)
Description

4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site.

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Features
Component Alleles
Molecular data

UASt regulatory sequences drive expression of a constitutively activated, ligand-independent form of N. The signal sequence and signal cleavage site of Dl (amino acid residues 1-27) are fused to N sequence from amino acid 1742 to the end of the coding sequence. A frameshift mutation in the N coding sequence causes premature termination after amino acid residue 2235 with 4 residues (RPPT) appended to the C terminus.

Allele
Reference(s)
Molecular data

4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments

construct_comment: Obtained from E. Giniger.

report comment: Notch protein produced is constitutively active.

Stocks (3)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (33)
Reported As
Symbol Synonym
P{UAS-Dl::N.DeltaECN}
P{UAS-Dl::N.act}
P{UAS-Dl::N.ΔECN}
P{w+mC = UAS-Dl::N.ΔECN}
UAS-N-DeltaEN
UAS-NΔ34a
UAS-NΔECN
UAS-Notch+Delta
UAS-Notch-intra
Secondary FlyBase IDs
  • FBtp0001310
References (58)