The double co-expression of InRK1409A.Scer\UAS and SmoxSDVD.Scer\UAS under the control of Scer\GAL4nub.PU suppresses the wing size and wing cell number defects induced by the single expression conditions. The co-expression of InRK1409A.Scer\UAS does not affect the decreased wing cell size induced by the expression of SmoxSDVD.Scer\UAS under the control of Scer\GAL4nub.PU.
The double co-expression of SmoxNIG.2262R and InRK1409A.Scer\UAS under the control of Scer\GAL4nub.PU (together with Dicer-2, for efficient RNAi) leads to a more severe decrease in wing size compared to all corresponding single expression conditions; the co-expression of InRK1409A.Scer\UAS suppresses the increased wing cell size, but does not affect the decreased wing cell number, induced by the expression of SmoxNIG.2262R under the control of Scer\GAL4nub.PU (together with Dicer-2, for efficient RNAi).
The overfeeding behavior observed in pre-starved adult flies expressing InRK1409A.Scer\UAS under the control of Scer\GAL4elav-C155 upon re-feeding is not suppressed by co-expression of fitScer\UAS.T:Ivir\HA1.
The decrease in preference for tryptone-supplemented food over a sucrose only food characteristic for unstarved adult flies expressing fitScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4elav-C155 in a two-way choice assay is suppressed by co-expression of InRK1409A.Scer\UAS.
Expression of GnmtmiRNA.Scer\UAS partially suppresses the increase in lifespan seen when InRK1409A.Scer\UAS is expressed under the control of Scer\GAL4αTub84B.Switch.PK (limited to the adult stages using RU486).
Co-expression of InRK1409A.Scer\UAS and LpinGD14004 under the control of Scer\GAL4Cg.PA leads to a majority of animals dying during larval development, with none reaching adulthood, and larvae exhibit a severely underdeveloped fat body, with greatly enlarged and rounded fat body cells that have enlarged nuclei and contain very small lipid droplets, as compared to controls.
One copy of foxoΔ94 partially rescues the sleep defects seen in flies expressing InRK1409A.Scer\UAS under the control of Scer\GAL4da.G32, specifically affecting day, but not night time phenotypes. Mutants show strongly reduced day activity and increased day sleep duration without changes in wakefulness (average activity per awake minute), and the number of day sleep bouts does not differ significantly from controls.
Expression of InRK1409A.Scer\UAS under the control of Scer\GAL4ppk.PG fully strongly suppresses the dendrite pruning defects seen in lin19EX mutant ddaC neuron clones at 16 hours after puparium formation. No difference is seen at the white prepupal stage.
Expression of InRK1409A.Scer\UAS completely suppresses the R8 photoreceptor phenotype seen when gogoScer\UAS.T1 is expressed under the control of Scer\GAL4GMR.PF. No blob-like structures are seen in the medulla M1 layer.
Expression of InRK1409A.Scer\UAS under the control of Scer\GAL4da.G32 in foxoΔ94 mutant flies results in an age-related increase in survival compared to controls, but this is less than in wild-type flies expressing InRK1409A.Scer\UAS.
foxoΔ94 mutant flies expressing InRK1409A.Scer\UAS under the control of Scer\GAL4da.G32 all survive for longer on food supplemented with 20mM paraquat compared to controls (but shorter compared to expression in a wild-type background).