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General Information
Symbol
Dmel\InRK1409A.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0156359
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-InRDN, UAS-InRK1409A, InRDN, UAS-InRDN, UAS-InR-DN, UAS-dInRDN, InR-DN, InR DN
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of a dominant negative form of InR (carries the amino acid replacement K1409A).

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Somatic clones expressing InRK1409A.UAS under the control of Scer\GAL4Tub.PU contain a smaller overall number of cells and intestinal stem cells in adult flies compared to controls, but maintain a wild-type ratio of stem cells/clone.

Expression of InRK1409A.UAS under the control of Scer\GAL4Su(H).GBE (in combination with tub-Gal80[ts] to restrict expression to the adult stage) leads to a decrease in the cell size of enteroblasts in adult guts when compared to controls.

Individuals expressing InRK1409A.UAS under the control of Scer\GAL4GMR11F02 show significant decreases in wing size and wing cell size and number, as compared to controls.

Posterior dorsocentral bristle neurons expressing InRK1409A.UAS under the control of Scer\GAL4pnr-MD237 show a significantly decreased terminal arbor territory and significantly fewer synapses (Syt1 puncta) on the contralateral collateral branch, as compared to controls.

Adulthood-generated midgut clones expressing InRK1409A.UAS under the control of Scer\GAL4Tub.PU are rare and very small, and do not show significant changes in the proportion of intestinal stem cells, as compared to control clones.

Expression of InRK1409A.UAS under the control of Scer\GAL4Tub.PU in MARCM clones leads to a significant decrease in midgut cell number when compared to control adults.

The expression of InRK1409A.Scer\UAS under the control of Scer\GAL4nub.PU leads to the adult wing showing a severe decrease in size, associated with a decrease in cell number and with a significant decrease in cell size, as compared to controls.

Expression of InRK1409A.Scer\UAS under the control of Scer\GAL4hh.PU results in decreased size of the posterior compartment in third instar larval wing discs.

Pre-starved adult flies expressing InRK1409A.Scer\UAS under the control of Scer\GAL4elav-C155 display an over-feeding behavior (significant increase in food consumption) upon re-feeding compared to controls. It does not however affect food choice of unstarved flies in a two-choice feeding assay: similar to controls, the flies display a preference for tryptone-supplemented (source of amino-acids) food over a sucrose-only food after sucrose pre-feeding.

Expressing InRK1409A.UAS under the control of Scer\GAL4en.PU has no effect on cell death in wing discs.

Expression of InRK1409A.Scer\UAS under the control of Scer\GAL4αTub84B.Switch.PK (limited to the adult stages using RU486) significantly increases lifespan.

Expression of InRK1409A.Scer\UAS under the control of Scer\GAL4Cg.PA does not interfere with normal development of the larval fat body or viability of the flies, and has no major effect on fat storage, but the average size of larval fat body cells is substantially reduced, as compared to controls.

Expression via Scer\GAL4wor.PA and Scer\GAL80ase.PN does not prevent the normal cell cycle exit of pupal neuroblasts.

Flies expressing InRK1409A.Scer\UAS under the control of Scer\GAL4da.G32 show typical circadian rhythmicity under 12:12 hour light:dark (LD) and constant dark (DD) conditions. However, in the mutants day activity is significantly increased, whereas night activity is significantly reduced ,a pattern that is maintained as the flies age. Wakefulness (average activity per awake minute) is not significantly altered, suggesting that they have a greater number of active periods during the day.

At all ages tested, flies expressing InRK1409A.Scer\UAS under the control of Scer\GAL4da.G32 sleep more at night and less by day than controls. In addition, they have fewer waking periods, and hence sleep bouts, during both day and night, and longer night sleep bouts than are seen in controls.

The increase in sleep fragmentation seen in control flies is less severe in flies expressing InRK1409A.Scer\UAS under the control of Scer\GAL4da.G32. Night sleep duration does not change and day sleep duration stays the same. Day and night sleep bouts do not increase with age.

Pupal volume is reduced compared to wild-type in animals expressing InRK1409A.Scer\UAS under the control of Scer\GAL4tub.PU.

Expression of InRK1409A.Scer\UAS under the control of two copies of Scer\GAL4ppk.PG produces dendrite pruning defects in ddaC neurons at 16 hours after puparium formation. No difference is seen in white prepupae.

Flies expressing InRK1409A.Scer\UAS under the control of Scer\GAL4sNPF-R.2.5 show significantly increased food consumption compared to controls.

No obvious photoreceptor phenotypes are seen when InRK1409A.Scer\UAS is expressed under the control of Scer\GAL4GMR.PF.

Expression of InRK1409A.Scer\UAS under the control of Scer\GAL4da.G32 in wild-type flies leads to a 10-15% increase in median lifespan and a 6-10% increase in maximum lifespan.

Expression of InRK1409A.Scer\UAS under the control of Scer\GAL4da.Switch.PT, induced by RU486, increases median lifespan by 24% and maximum lifespan by 5%.

Scer\GAL4da.G32>InRK1409A.Scer\UAS females show reduced egg laying compared with controls.

Flies expressing InRK1409A.Scer\UAS under the control of Scer\GAL4da.G32 display a delay in egg-adult development time by over 24 hours compared to controls. These flies also show a significant reduction in both body weight and wing size.

Flies expressing InRK1409A.Scer\UAS under the control of Scer\GAL4da.G32 all survive for significantly longer on food supplemented with 20mM paraquat compared to controls.

Flies expressing InRK1409A.Scer\UAS under the control of Scer\GAL4da.G32 survive for longer in the presence of DTT, compared to controls.

Expression of InRK1409A.Scer\UAS in the developing eye under the control of Scer\GAL4ey.PH inhibits growth, leading to a smaller eye than in controls.

Central nervous system neuroblasts of fed larvae expressing InRK1409A.Scer\UAS under the control of Scer\GAL4nab show a reduction in EdU incorporation compared to controls.

Female flies expressing InRK1409A.Scer\UAS ubiquitously under the control of Scer\GAL4da.G32 show a significantly reduced re-mating rate 24 hours after mating to wild-type flies, compared to control flies.

Female flies expressing InRK1409A.Scer\UAS ubiquitously post-development under the control of Scer\GAL4Act5C.Switch.PR show a significantly reduced re-mating rate 24 hours after mating to wild-type flies, compared to control flies.

Compared with controls, the age-related dysplasia of the intestinal epithelium is strongly reduced in flies expressing InRK1409A.Scer\UAS under the control of Scer\GAL4esg-NP5130 and Scer\GAL80ts.αTub84B.

Compared with controls, female flies expressing InRK1409A.Scer\UAS under the control of Scer\GAL4bun-GSG5961 show a moderate, but significant, reduction in intestinal cell proliferation at old age. These flies are significantly longer lived when exposed to RU486 than isogenic siblings exposed to mock treatment.

Expression of InRK1409A.Scer\UAS driven by Scer\GAL4ap-md544 in dorsal cells of the wing causes a phenotype in which the wing bends upward.

Mushroom body neuroblasts persist slightly longer than normal in animals expressing InRK1409A.Scer\UAS under the control of Scer\GAL4wor.PA.

Female flies expressing InRK1409A.Scer\UAS under the control of Scer\GAL4da.G32 weigh significantly less, and lay fewer eggs, than sibling controls. Adult fat body morphology is altered in these animals, displaying a misshapen and shrunken appearance. Mutant flies are short-lived. Very few mutant male flies eclose, and those that do have a much reduced life-span.

The affect of expressing InRK1409A.Scer\UAS under the control of Scer\GAL4da.G32 is less pronounced but still significant if the female flies are infected with Wolbachia. Reductions in body weight and the number of eggs laid is observed, however infected female flies display normal adult fat body morphology. Infected mutant flies have a large extension of median and maximum life-span. Infected mutant male flies display a slight reduction in median lifespan.

Female flies infected with Wolbachia expressing InRK1409A.Scer\UAS in the adult fat body under the control of Scer\GAL4Switch1.106 display a normal lifespan. Removal of the Wolbachia from these flies results in an extension of lifespan.

The oenocytes of larvae expressing InRK1409A.Scer\UAS under the control of Scer\GAL4fat contain lipid droplets regardless of whether the larvae are fed a standard diet or are deprived of food (wild-type oenocytes only accumulate lipid droplets when they are starved)

Overexpression of InRK1409A.Scer\UAS under the control of Scer\GAL4796 does not induce any significant effect.

When fed ad libitum, third instar larvae expressing InRK1409A.Scer\UAS, under the control of Scer\GAL4NPFR1.6.6 display hyperactive feeding on solid food, similar to control third instar larvae deprived of food for 40 minutes. These third instar larvae do not exhibit significant changes in the intake rate of the richer liquid food, relative to controls.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
NOT Enhanced by
Suppressed by
Statement
Reference

InRK1409A.UAS, Scer\GAL4da.Switch.PT has long lived | RU486 conditional phenotype, suppressible by foxoΔ94

NOT suppressed by
Enhancer of
NOT Enhancer of
Suppressor of
Statement
Reference
NOT Suppressor of
Other
Phenotype Manifest In
Enhanced by
NOT Enhanced by
Statement
Reference
Suppressed by
Statement
Reference
Enhancer of
NOT Enhancer of
Suppressor of
NOT Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference

Diap14 heterozygosity ameliorates the wing defects induced by the expression of InRK1409A.UAS under the control of Scer\GAL4GMR11F02 (decreased wing size and decreases in wing cell size and number).

The double co-expression of InRK1409A.Scer\UAS and SmoxSDVD.Scer\UAS under the control of Scer\GAL4nub.PU suppresses the wing size and wing cell number defects induced by the single expression conditions. The co-expression of InRK1409A.Scer\UAS does not affect the decreased wing cell size induced by the expression of SmoxSDVD.Scer\UAS under the control of Scer\GAL4nub.PU.

The double co-expression of SmoxNIG.2262R and InRK1409A.Scer\UAS under the control of Scer\GAL4nub.PU (together with Dicer-2, for efficient RNAi) leads to a more severe decrease in wing size compared to all corresponding single expression conditions; the co-expression of InRK1409A.Scer\UAS suppresses the increased wing cell size, but does not affect the decreased wing cell number, induced by the expression of SmoxNIG.2262R under the control of Scer\GAL4nub.PU (together with Dicer-2, for efficient RNAi).

The overfeeding behavior observed in pre-starved adult flies expressing InRK1409A.Scer\UAS under the control of Scer\GAL4elav-C155 upon re-feeding is not suppressed by co-expression of fitScer\UAS.T:Ivir\HA1.

The decrease in preference for tryptone-supplemented food over a sucrose only food characteristic for unstarved adult flies expressing fitScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4elav-C155 in a two-way choice assay is suppressed by co-expression of InRK1409A.Scer\UAS.

Expression of GnmtmiRNA.Scer\UAS partially suppresses the increase in lifespan seen when InRK1409A.Scer\UAS is expressed under the control of Scer\GAL4αTub84B.Switch.PK (limited to the adult stages using RU486).

Co-expression of InRK1409A.Scer\UAS and LpinGD14004 under the control of Scer\GAL4Cg.PA leads to a majority of animals dying during larval development, with none reaching adulthood, and larvae exhibit a severely underdeveloped fat body, with greatly enlarged and rounded fat body cells that have enlarged nuclei and contain very small lipid droplets, as compared to controls.

One copy of foxoΔ94 partially rescues the sleep defects seen in flies expressing InRK1409A.Scer\UAS under the control of Scer\GAL4da.G32, specifically affecting day, but not night time phenotypes. Mutants show strongly reduced day activity and increased day sleep duration without changes in wakefulness (average activity per awake minute), and the number of day sleep bouts does not differ significantly from controls.

Expression of InRK1409A.Scer\UAS dramatically suppresses the dendrite pruning defects seen in ddaC neurons expressing lin19GD9650 under the control of Scer\GAL4ppk.PG at 16 hours after pupariation.

Expression of InRK1409A.Scer\UAS suppresses the dendrite pruning defects seen in ddaC neurons expressing skpAGD9251 under the control of Scer\GAL4ppk.PG at 16 hours after pupariation.

Expression of InRK1409A.Scer\UAS under the control of Scer\GAL4ppk.PG fully strongly suppresses the dendrite pruning defects seen in lin19EX mutant ddaC neuron clones at 16 hours after puparium formation. No difference is seen at the white prepupal stage.

Expression of InRK1409A.Scer\UAS does not affect the R8 axon stopping phenotype seen when gogoDDD.Scer\UAS is expressed under the control of Scer\GAL4GMR.PF.

Expression of InRK1409A.Scer\UAS completely suppresses the R8 photoreceptor phenotype seen when gogoScer\UAS.T1 is expressed under the control of Scer\GAL4GMR.PF. No blob-like structures are seen in the medulla M1 layer.

Expression of InRK1409A.Scer\UAS under the control of Scer\GAL4da.G32 does not affect the viability of foxoΔ94 flies.

Expression of InRK1409A.Scer\UAS under the control of Scer\GAL4da.G32 in foxoΔ94 mutant flies results in an age-related increase in survival compared to controls, but this is less than in wild-type flies expressing InRK1409A.Scer\UAS.

A foxoΔ94 mutant background suppresses the extension in lifespan seen upon RU486-induced expression of InRK1409A.Scer\UAS under the control of Scer\GAL4da.Switch.PT.

Adult onset ubiquitous expression of InRK1409A.Scer\UAS under the control of the RU486-inducible Scer\GAL4da.Switch.PT has no effect on the viability of foxoΔ94 flies.

foxoΔ94 mutant females expressing InRK1409A.Scer\UAS under the control of Scer\GAL4da.G32 show reduced egg laying compared with controls.

foxoΔ94 mutant females expressing InRK1409A.Scer\UAS under the control of Scer\GAL4da.Switch.PT treated with RU486 lay significantly fewer eggs than their uninduced controls.

foxoΔ94 mutant flies expressing InRK1409A.Scer\UAS under the control of Scer\GAL4da.G32 all survive for longer on food supplemented with 20mM paraquat compared to controls (but shorter compared to expression in a wild-type background).

A foxoΔ94 background suppresses the DTT resistance seen in flies expressing InRK1409A.Scer\UAS under the control of Scer\GAL4da.G32.

A foxoΔ94 background fully suppresses the tissue-restricted growth inhibition seen in the eye upon expression of InRK1409A.Scer\UAS under the control of Scer\GAL4ey.PH.

Removal of foxo through a foxoΔ94 background does not suppress the developmental delay or reduced body size seen in flies expressing InRK1409A.Scer\UAS under the control of Scer\GAL4da.G32.

Co-expression of Sesnd04539 with InRK1409A.Scer\UAS driven by Scer\GAL4ap-md544 enhances the wing-bending phenotype of InRK1409A.Scer\UAS.

Sesn8A11 does not enhance the growth suppression resulting from Scer\GAL4ap-md544-driven InRK1409A.Scer\UAS expression in the wing.

Xenogenetic Interactions
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Complementation and Rescue Data
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Mutant
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Stocks (3)
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External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
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    References (49)