FlyBase Allele Report: Dmel\Diap1[4]

General Information

Symbol

Dmel\Diap1⁴

Species

D. melanogaster

Name

FlyBase ID

FBal0040009

Feature type

allele

Associated gene

Dmel\Diap1

Associated Insertion(s)

Carried in Construct

Also Known As

th⁴

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

loss of function allele

Mutagen

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Choi et al., 2006)

loss of function allele

(Lisi et al., 2000)

Mutagen

Progenitor genotype

Cytology

Description

Amino acid replacement: H283Y.

(Wilson et al., 2002)

Amino acid replacement: H283Y. This mutation is within the second BIR domain.

(Goyal et al., 2000)

Amino acid replacement: H283Y. H283 falls in the BIR domain.

(Lisi et al., 2000)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:16,039,142..16,039,142 [-]

Nucleotide change:

C16039142T

Amino acid change:

Reported amino acid change:

H283Y

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Goyal et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Huntington's disease

modeled by Hsap\HTT^{128Q.1-336.UAS}

(Branco et al., 2008)

exacerbates olivopontocerebellar atrophy

modeled by Hsap\ATXN1^82Q.UAS

(Branco et al., 2008)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

border follicle cell (with Diap1^21-2s)

(Geisbrecht and Montell, 2004)

border follicle cell | somatic clone

(Geisbrecht and Montell, 2004)

neuron

(Kuo et al., 2006)

wing

(Shinoda et al., 2019)

Detailed Description

Statement

Reference

Diap1⁴ heterozygotes show slight but significant increases in wing size and wing cell numbers, despite of no changes in cell numbers, as compared to controls.

(Shinoda et al., 2019)

As in wild type, th¹/th⁴ mutant bursCCAP neurons undergo programmed cell death shortly after eclosion.

(Lee et al., 2013)

th⁴ heterozygotes do not exhibit defects in dendrite pruning.

(Rumpf et al., 2011)

No retinal phenotypes are seen in th⁴ mutant flies.

(Branco et al., 2008)

Mutants heterozygous for th⁴ exhibit normal scheduled death of Crz-expressing neurons.

(Choi et al., 2006)

th⁴ mutant C4da neuron clones exhibit an increase in cell death and die prior to metamorphosis.

(Kuo et al., 2006)

13% of th⁴/th^21-2s egg chambers have border follicle cell migration defects. Homozygous follicle cells clones result in border follicle cell migration defects.

(Geisbrecht and Montell, 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Diap1⁴ has abnormal size | adult stage phenotype, suppressible by InR^K1409A.UAS/Scer\GAL4^GMR11F02

(Shinoda et al., 2019)

Diap1⁴ has increased cell number | dominant | adult stage phenotype, suppressible by InR^K1409A.UAS/Scer\GAL4^GMR11F02

(Shinoda et al., 2019)

Enhancer of

Statement

Reference

Diap1[+]/Diap1⁴ is an enhancer of increased cell death | larval stage phenotype of RpS3^Plac92

(Wada et al., 2021)

Diap1[+]/Diap1⁴ is an enhancer of visible | adult stage phenotype of Dronc^UAS.cSa, Scer\GAL4^GMR.PFa

(Sinenko, 2017)

Diap1[+]/Diap1⁴ is an enhancer of abnormal eye color phenotype of Dronc^UAS.cSa, Scer\GAL4^GMR.PFa

(Sinenko, 2017)

Diap1[+]/Diap1⁴ is an enhancer of visible | adult stage phenotype of Dronc^{S130A.UAS.cSa}, Scer\GAL4^GMR.PFa

(Sinenko, 2017)

Diap1[+]/Diap1⁴ is an enhancer of abnormal neuroanatomy | adult stage | progressive phenotype of ebi^1-334.GMR

(Lim et al., 2012)

Diap1⁴/th[+] is an enhancer of lethal | heat sensitive phenotype of Scer\GAL4^GMR.PF, scny^GS13668

(Ribaya et al., 2009)

Diap1⁴/th[+] is an enhancer of visible | heat sensitive phenotype of Scer\GAL4^GMR.PF, scny^GS13668

(Ribaya et al., 2009)

Diap1⁴/th[+] is an enhancer of visible phenotype of Scer\GAL4^sev.PU, Zzzz\CTG^i480.UAS.cGa

(Garcia-Lopez et al., 2008)

Diap1⁴ is an enhancer of increased cell death phenotype of shtd¹

(Tanaka-Matakatsu et al., 2007)

Diap1⁴ is an enhancer of visible phenotype of H^UAS.cMa, Scer\GAL4^GMR.PF

(Müller et al., 2005)

Diap1⁴ is an enhancer of visible phenotype of Dronc^pro.UAS, Scer\GAL4^GMR.PF

(Wilson et al., 2002)

Diap1⁴ is an enhancer of abnormal cell death phenotype of rpr^GMR.PW

(Lisi et al., 2000)

Diap1⁴ is an enhancer of abnormal cell death phenotype of hid^GMR.PG

(Lisi et al., 2000)

Diap1⁴ is an enhancer of abnormal cell death phenotype of grim^GMR.PC

(Lisi et al., 2000)

Suppressor of

Statement

Reference

Diap1[+]/Diap1⁴ is a suppressor | partially of abnormal size | adult stage phenotype of InR^K1409A.UAS, Scer\GAL4^GMR11F02

(Shinoda et al., 2019)

Diap1[+]/Diap1⁴ is a suppressor | partially of decreased cell size | adult stage phenotype of InR^K1409A.UAS, Scer\GAL4^GMR11F02

(Shinoda et al., 2019)

Diap1[+]/Diap1⁴ is a suppressor | partially of decreased cell number | adult stage phenotype of InR^K1409A.UAS, Scer\GAL4^GMR11F02

(Shinoda et al., 2019)

Diap1⁴/th[+] is a suppressor of decreased cell death | pupal stage phenotype of Scer\GAL4^19-12, TER94^{E375Q.E575Q.UAS}

(Rumpf et al., 2011)

Diap1⁴/th[+] is a suppressor | partially of abnormal neuroanatomy phenotype of Scer\GAL4^ppk.PG, TER94^{E375Q.E575Q.UAS}

(Rumpf et al., 2011)

Diap1⁴ is a suppressor of increased cell death phenotype of Prx4^AKP.Ub.GMR

(Tenev et al., 2002)

Other

Statement

Reference

Diap1⁴, RpS3^Plac92 has visible | adult stage phenotype

(Wada et al., 2021)

Diap1⁴, RpS3^Plac92 has decreased size | adult stage phenotype

(Wada et al., 2021)

Phenotype Manifest In

Suppressed by

Statement

Reference

Diap1⁴ has wing phenotype, suppressible by InR^K1409A.UAS/Scer\GAL4^GMR11F02

(Shinoda et al., 2019)

Enhancer of

Statement

Reference

Diap1[+]/Diap1⁴ is an enhancer of wing disc | larval stage phenotype of RpS3^Plac92

(Wada et al., 2021)

Diap1[+]/Diap1⁴ is an enhancer of eye phenotype of Dronc^UAS.cSa, Scer\GAL4^GMR.PFa

(Sinenko, 2017)

Diap1[+]/Diap1⁴ is an enhancer of retina | progressive phenotype of ebi^1-334.GMR

(Lim et al., 2012)

Diap1⁴/th[+] is an enhancer of eye | heat sensitive phenotype of Scer\GAL4^GMR.PF, scny^GS13668

(Ribaya et al., 2009)

Diap1⁴/th[+] is an enhancer of retina phenotype of Hsap\HTT^{128Q.1-336.UAS}, Scer\GAL4^GMR.PU

(Branco et al., 2008)

Diap1⁴/th[+] is an enhancer of retina phenotype of Hsap\ATXN1^82Q.UAS, Scer\GAL4^GMR.PU

(Branco et al., 2008)

Diap1⁴/th[+] is an enhancer of eye phenotype of Scer\GAL4^sev.PU, Zzzz\CTG^i480.UAS.cGa

(Garcia-Lopez et al., 2008)

Diap1⁴ is an enhancer of eye phenotype of Scer\GAL4^hs.2sev, mbl^C.UAS

(Vicente-Crespo et al., 2008)

Diap1⁴ is an enhancer of eye phenotype of shtd¹

(Tanaka-Matakatsu et al., 2007)

Diap1⁴ is an enhancer of ommatidium phenotype of shtd¹

(Tanaka-Matakatsu et al., 2007)

Diap1⁴ is an enhancer of eye phenotype of H^UAS.cMa, Scer\GAL4^GMR.PF

(Müller et al., 2005)

Diap1⁴/th[+] is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, egr^GS9830

(Igaki et al., 2002)

Diap1⁴ is an enhancer of eye phenotype of rpr^AVA.Ub.GMR

(Tenev et al., 2002)

Diap1⁴ is an enhancer of eye phenotype of rpr^AKP.Ub.GMR

(Tenev et al., 2002)

Diap1⁴ is an enhancer of eye phenotype of Dronc^pro.UAS, Scer\GAL4^GMR.PF

(Wilson et al., 2002)

Diap1⁴ is an enhancer of phenotype of hid^GMR.PG

(Goyal et al., 2000)

Diap1⁴ is an enhancer of phenotype of rpr^GMR.PW

(Goyal et al., 2000)

Diap1⁴ is an enhancer of eye phenotype of rpr^GMR.PW

(Lisi et al., 2000)

Diap1⁴ is an enhancer of eye phenotype of hid^GMR.PG

(Lisi et al., 2000, Hay et al., 1995)

Diap1⁴ is an enhancer of eye phenotype of grim^GMR.PC

(Lisi et al., 2000)

Diap1⁴ is an enhancer of eye phenotype of rpr^GMR.PH

(Hay et al., 1995)

Suppressor of

Statement

Reference

Diap1[+]/Diap1⁴ is a suppressor | partially of wing phenotype of InR^K1409A.UAS, Scer\GAL4^GMR11F02

(Shinoda et al., 2019)

Diap1⁴/th[+] is a suppressor of larval multidendritic class IV neuron phenotype of Scer\GAL4^ppk.PG, TER94^{E375Q.E575Q.UAS}

(Rumpf et al., 2011)

Diap1⁴/th[+] is a suppressor of dendrite phenotype of Scer\GAL4^ppk.PG, TER94^{E375Q.E575Q.UAS}

(Rumpf et al., 2011)

Diap1⁴ is a suppressor of eye phenotype of Prx4^AKP.Ub.GMR

(Tenev et al., 2002)

Other

Statement

Reference

Diap1⁴, RpS3^Plac92 has wing phenotype

(Wada et al., 2021)

Diap1[+]/Diap1⁴, Dronc^{S130A.UAS.cSa}, Scer\GAL4^GMR.PFa has eye phenotype

(Sinenko, 2017)

Additional Comments

Genetic Interactions

Statement

Reference

Diap1⁴ heterozygosity ameliorates the wing defects induced by the expression of InR^K1409A.UAS under the control of Scer\GAL4^GMR11F02 (decreased wing size and decreases in wing cell size and number).

(Shinoda et al., 2019)

The photoreceptor depigmentation phenotype characteristic for adult flies expressing Dronc^UAS.cSa under the control of Scer\GAL4^GMR.PFa is strongly exacerbated by combination with a single copy of Diap1⁴.

(Sinenko, 2017)

Diap1⁴/+ enhances the late-onset retinal degeneration phenotype of ebi^1-334.GMR/+ flies.

(Lim et al., 2012)

A th⁴ heterozygous mutant background suppresses the class IV pruning defects seen upon expression of TER94^{E375Q.E575Q.Scer\UAS} under the control of Scer\GAL4^ppk.PG. Lowering th levels also sensitises cells to stress-induced apoptosis, and a high proportion of TER94^{E375Q.E575Q.Scer\UAS}-expressing neurons in a th⁴ heterozygous background undergo apoptosis during early metamorphosis (approximately 60% at 15 hours after pupal formation).

(Rumpf et al., 2011)

The semi-lethality seen at 22[o]C in animals expressing scny^GS13668 under the control of Scer\GAL4^GMR.PF is enhanced by th⁴/+, so that none of the double mutant animals eclose.

th⁴/+ enhances the severity of the rough eye phenotype caused by expression of scny^GS13668 under the control of Scer\GAL4^GMR.PF at 18[o]C.

(Ribaya et al., 2009)

A th⁴ mutant background enhances the moderately rough eye phenotype observed upon expression of mbl^C.Scer\UAS under the control of Scer\GAL4^hs.2sev.

(Vicente-Crespo et al., 2008)

The addition of th⁴/+ to egr^GS9830, Scer\GAL4^GMR.PF animals leads to a completely ablated eye phenotype, an enhancement of the egr^GS9830, Scer\GAL4^GMR.PF phenotype.

(Igaki et al., 2002)

Addition of this allele strongly enhances the phenotype seen in rpr^GMR.PW and W^GMR.PG flies.

(Goyal et al., 2000)

Xenogenetic Interactions

Statement

Reference

One copy of th⁴ enhances the retinal phenotype seen when Hsap\HTT^{128Q.1-336.Scer\UAS} is expressed under the control of Scer\GAL4^GMR.PU.

One copy of th⁴ enhances the retinal phenotype seen when Hsap\ATXN1^82Q.Scer\UAS is expressed under the control of Scer\GAL4^GMR.PU.

(Branco et al., 2008)

Complementation and Rescue Data

Fails to complement

Diap1^I1

(Kennison, 2008.10.30)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

5053

Diap1⁴/TM6C, cu¹ Sb¹ ca¹

Kyoto

108011

Diap1⁴/TM6C, cu¹ Sb¹ ca¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

DIAP1⁴

(Sinenko, 2017)

DIAP1^LOF

(Branco et al., 2008)

Diap1⁴

(Yamada et al., 2023, Wang and Baker, 2019)

Th4

(Silke and Vaux, 2001)

diap1⁴

(Wang and Baker, 2015)

th⁴

(Wada et al., 2021, Shinoda et al., 2019, Lee et al., 2013, Lim et al., 2012, Rumpf et al., 2011, Kondo et al., 2009, Ribaya et al., 2009, Branco et al., 2008, Garcia-Lopez et al., 2008, Kennison, 2008.10.30, Vicente-Crespo et al., 2008, Wang et al., 2008, Tanaka-Matakatsu et al., 2007, Choi et al., 2006, Kuo et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (31)

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