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General Information
Symbol
Dmel\grimGMR.PC
Species
D. melanogaster
Name
glass multimer reporter construct of Chen
FlyBase ID
FBal0051534
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
GMR-grim, P[GMR-grim]
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

GMR regulatory sequences drive expression of an EcoRI fragment of grim cDNA.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Flies carrying one copy of grimGMR.PC show depigmentation, mild roughness and slight reduction in the size of adult eyes; in flies carrying two copies of the transgene, only a sliver of an eye remains and is largely depigmented.

Adult flies expressing grimGMR.PC show retinal degeneration phenotype.

One copy of grimGMR.PC has no effect on the eye in an otherwise wild-type background. Flies carrying two copies of grimGMR.PC show a reduction in eye size.

grimGMR.PC eyes are small owing to cell death of a large percentage of the developing cells of the eye.

Increased cell death is seen in grimGMR.PC third instar eye discs.

WGMR.PG eyes are ablated.

Flies expressing grimGMR.PC have a rough eye phenotype.

Flies carrying grimGMR.PC show almost complete ablation of the eye.

Adults expressing grimGMR.PC show a reduction in eye size compared to wild type.

Expression of the grimGMR.PC transgene in postmitotic cells of the eye disc leads to small, rough eyes.

Flies carrying grimGMR.PC have rough and reduced eyes.

Flies expressing grimGMR.PC have small, rough eyes.

grimGMR.PC flies have small, rough eyes. In third instar eye discs of these animals, abnormally high levels of cell death occur posterior to the morphogenetic furrow.

grimGMR.PC results in a dosage dependent eye degeneration phenotype.

grimGMR.PC has little or no effect on eye suize in heterozygotes.

grimGMR.PC has little or no effect on eye size in heterozygotes.

Eye is small and roughened.

grimGMR.PC flies have a mild but easily detectable eye phenotype.

Two copies of grimGMR.PC in an otherwise wild-type background completely eliminate the ommatidia.

Causes a dose-dependent mild eye ablation phenotype.

Induces cell death. This phenotype is not modified by modulating the Ras/MAPK pathway, or by reducing W gene dosage.

Single copy of P{GMR-grim} causes disordering of ommatidia and mechanosensory bristles. Two copies causes a complete elimination of ommatidia with only a few bristles remaining.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference

grimGMR.PC has visible phenotype, enhanceable by PSR[+]/JMJD6FM1

NOT Enhanced by
Statement
Reference
Suppressed by
Statement
Reference

grimGMR.PC has visible | adult stage phenotype, suppressible | partially by Usp15-31KG04149/Usp15-31[+]

grimGMR.PC has abnormal eye color phenotype, suppressible | partially by Usp15-31KG04149/Usp15-31[+]

grimGMR.PC has abnormal size | adult stage phenotype, suppressible | partially by Usp15-31KG04149/Usp15-31[+]

grimGMR.PC has visible phenotype, suppressible by pnut[+]/pnutXP

grimGMR.PC has visible phenotype, suppressible by pnut[+]/pnut1

grimGMR.PC has visible phenotype, suppressible | partially by klu[+]/kluunspecified

grimGMR.PC has visible phenotype, suppressible by DarkCD4

NOT suppressed by
Statement
Reference
Enhancer of
Statement
Reference
Other
Phenotype Manifest In
Enhanced by
Statement
Reference

grimGMR.PC has eye phenotype, enhanceable by PSR[+]/JMJD6FM1

grimGMR.PC has eye phenotype, enhanceable by JMJD6FM1/JMJD6FM1

grimGMR.PC has eye phenotype, enhanceable by Diap15

grimGMR.PC has eye phenotype, enhanceable by Diap181.03

grimGMR.PC has eye phenotype, enhanceable by Diap19

grimGMR.PC has eye phenotype, enhanceable by Diap1109.07

grimGMR.PC has eye phenotype, enhanceable by Diap14

grimGMR.PC has eye phenotype, enhanceable by Diap16B

grimGMR.PC has eye phenotype, enhanceable by Diap17

NOT Enhanced by
Statement
Reference
Suppressed by
Statement
Reference

grimGMR.PC has eye phenotype, suppressible by DarkKK104215/Scer\GAL4GMR.PFa

grimGMR.PC has eye phenotype, suppressible | partially by Usp15-31KG04149/Usp15-31[+]

grimGMR.PC has eye phenotype, suppressible | partially by BuffyH37

grimGMR.PC has eye phenotype, suppressible by pnut[+]/pnutXP

grimGMR.PC has eye phenotype, suppressible by pnut[+]/pnut1

grimGMR.PC has ommatidium phenotype, suppressible | partially by klu[+]/kluunspecified

grimGMR.PC has eye phenotype, suppressible | somatic clone by hpoMGH1

grimGMR.PC has eye phenotype, suppressible by Diap1SL

grimGMR.PC has ommatidium phenotype, suppressible by DarkCD4

grimGMR.PC has eye phenotype, suppressible by DarkCD4

grimGMR.PC has eye phenotype, suppressible by Df(1)su(s)R194

NOT suppressed by
Statement
Reference

grimGMR.PC has eye phenotype, non-suppressible by DebclE26

grimGMR.PC has eye phenotype, non-suppressible by Debcl59

grimGMR.PC has eye phenotype, non-suppressible by TER94[+]/TER9426-8

grimGMR.PC has eye phenotype, non-suppressible by Darkk11502

grimGMR.PC has eye phenotype, non-suppressible by Ras85DN17.sev

grimGMR.PC has eye phenotype, non-suppressible by Ras85DV12.sev

Enhancer of
Statement
Reference
NOT Enhancer of
Statement
Reference

grimGMR.PC is a non-enhancer of phenotype of Dricefl.GMR

Other
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

The eye phenotype (partial depigmentation, rough surface, slight size reduction) characteristic for adult flies carrying one copy of grimGMR.PC is significantly improved by expression of Usp15-31KK101035 and even more strongly by that of DarkKK104215 driven by Scer\GAL4GMR.PFa. Similarly, the even stronger eye phenotype observed in flies carrying two copies of grimGMR.PC is partially suppressed by combination with Usp15-31KG04149 in either homozygous or heterozygous state.

Expression of ManfScer\UAS.cPa under the control of Scer\GAL4Hml.Δ reduces the severity of the retinal degeneration phenotype characteristic for adult flies expressing grimGMR.PC.

Co-expression of grimGMR.PC and DUBAIGD14040 (expressed under the control of Scer\GAL4GMR.PFa) results in a significant loss of pigment cells in the eye.

grimGMR.PC DUBAIKG07439/+ flies show loss of pigment cells in the eye.

Flies carrying one copy of grimGMR.PC and expressing one of either UbpyKK100733 or Usp12-46KK108313 under the control of Scer\GAL4GMR.PFa do not show a mutant eye phenotype.

Flies carrying one copy of grimGMR.PC and also heterozygous for CG5789KG07791 do not show a mutant eye phenotype.

The eye phenotype caused by two copies of grimGMR.PC is enhanced by DUBAIKG07439/DUBAIKG07439.

Ras85DR68Q very mildly suppresses grimGMR.PC-induced cell death although this is suspected to be due to suppression of endogenous W activity and not grim or rpr activity.

The cell death seen in grimGMR.PC eyes is abrogated in a BuffyH37 but not in a debclE26 background.

Co-expression of PSRScer\UAS.cKa under the control of Scer\GAL4GMR.PF suppresses the rough eye phenotype seen in flies expressing grimGMR.PC.

The rough eye phenotype caused by grimGMR.PC is enhanced by one copy of PSRFM1 and is further enhanced by two copies of PSRFM1.

The loss of eye tissue that is caused by grimGMR.PC is partially suppressed if the eye is also homozygous for Uba1B2.

The small, rough eye phenotype of grimGMR.PC flies is partially suppressed by the expression of mir-278KN448.mirvana-1.Scer\UAS under the control of Scer\GAL4GMR.PF and strongly suppressed by mir-278KN447.Scer\UAS under the control of Scer\GAL4GMR.PF.

The eye phenotype of flies expressing grimGMR.PC is not enhanced by a mir-278mirvana-1 or a mir-278mirvana-1/Df(2R)Exel7137 background.

The severity of the grimGMR.PC eye phenotype is suppressed by "dronc[d5]" animals (Df(3L)NcZ in which CG6685 function, but not Nc function, is rescued by P{CG6685+tDa}).

The reduced eye phenotype caused by expression of grimGMR.PC is significantly suppressed by pnut1/+ or pnutXP/+.

The eye phenotype caused by grimGMR.PC is mildly but consistently suppressed by kluunspecified/+.

The small, rough eye phenotype of grimGMR.PC flies is suppressed when somatic clones of hpoMGH1 homozygous cells are generated throughout the eye discs using Scer\FLP1ey.PN with P{neoFRT}42D. In the eye discs from these animals, the abnormal levels of cell death posterior to the morphogenetic furrow seen in grimGMR.PC eye discs are suppressed in the hpoMGH1 mutant tissue.

The addition of th5 to grimGMR.PC heterozygotes leads to an enhancement of retinal apoptosis. The subsequent addition of ArkCD4/ArkCD4 suppresses that enhancement.

The addition of grimGMR.PC to Dcp-1fl.GMR flies show a strong enhancement of the eye phenotype seen in Dcp-1fl.GMR flies. The addition of grimGMR.PC to Icefl.GMR flies did not show a very effective enhancement of the eye phenotype seen in Icefl.GMR flies.

The grimGMR.PC ommatidia phenotype is suppressed if the flies are also homozygous for ArkCD4; the eyes retain a substantial amount of retinal tissue with many surviving ommatidia.

Arkk11502 has little effect on the eye phenotype caused by grimGMR.PC.

The grimGMR.PC eye ablation phenotype is not particularly affected by either Ras85DV12.sev and Ras85DN17.sev.

Cell-killing in the eye induced by grimGMR.PC is suppressed by Df(1)su(s)R194.

Simultaneous expression of P{GMR-grim} and P{GMRP35} restores wild type size and organisation of the eye.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
grimGMR.PC
Name Synonyms
glass multimer reporter construct of Chen
Secondary FlyBase IDs
    References (35)