FlyBase Allele Report: Dmel\Diap1[V85M.UAS]

General Information

Symbol

Dmel\Diap1^V85M.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0103506

Feature type

allele

Associated gene

Dmel\Diap1

Associated Insertion(s)

Carried in Construct

P{UAS-Diap1.V85M}

Key Links

Transgenic product class

missense_variant

(Lisi et al., 2000)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Lisi et al., 2000)

Mutagen

in vitro construct

(Lisi et al., 2000)

Progenitor genotype

Diap1^SL

(Lisi et al., 2000)

Carried in construct

P{UAS-Diap1.V85M}

Cytology

Description

The Diap1 coding region carrying the Diap1^SL mutation (amino acid replacement V85M) is expressed under the control of UASt regulatory sequences.

(Lisi et al., 2000)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Diap1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^da.PU/Diap1^V85M.UAS is a suppressor of majority die during third instar larval stage phenotype of Usp5²

(Kovacs et al., 2015)

Scer\GAL4^da.PU/Diap1^V85M.UAS is a suppressor | partially of increased cell death | larval stage phenotype of Usp5²

(Kovacs et al., 2015)

Diap1^V85M.UAS/Scer\GAL4^GMR.PU is a suppressor of abnormal cell death phenotype of rpr^GMR.PW

(Lisi et al., 2000)

Diap1^V85M.UAS/Scer\GAL4^GMR.PU is a suppressor of abnormal cell death phenotype of grim^GMR.PC

(Lisi et al., 2000)

Diap1^V85M.UAS/Scer\GAL4^GMR.PU is a suppressor | partially of abnormal cell death phenotype of hid^GMR.PG

(Lisi et al., 2000)

NOT Suppressor of

Statement

Reference

Scer\GAL4^da.PU/Diap1^V85M.UAS is a non-suppressor of lethal phenotype of Usp5²

(Kovacs et al., 2015)

Other

Statement

Reference

Diap1^V85M.UAS, Scer\GAL4^da.PU, Usp5² has majority die during pupal stage phenotype

(Kovacs et al., 2015)

Phenotype Manifest In

Suppressor of

Statement

Reference

Scer\GAL4^Tub.PU/Diap1^V85M.UAS is a suppressor of wing disc | somatic clone | cell autonomous phenotype of yki^B5

(Neto-Silva et al., 2010)

Diap1^V85M.UAS/Scer\GAL4^GMR.PU is a suppressor of eye phenotype of grim^GMR.PC

(Lisi et al., 2000)

Diap1^V85M.UAS/Scer\GAL4^GMR.PU is a suppressor | partially of eye phenotype of hid^GMR.PG

(Lisi et al., 2000)

Diap1^V85M.UAS/Scer\GAL4^GMR.PU is a suppressor of eye phenotype of rpr^GMR.PW

(Lisi et al., 2000)

NOT Suppressor of

Statement

Reference

Myc^UAS.cZa, Scer\GAL4^Tub.PU, Diap1^V85M.UAS is a non-suppressor of wing disc | somatic clone | cell autonomous phenotype of yki^B5

(Neto-Silva et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Diap1^{V85M.Scer\UAS} under the control of Scer\GAL4^da.PU partially suppresses the lethality and apoptosis seen in Usp5² mutants, shifting the third instar larval lethality to the pupal stage. The increased apoptotic index in Usp5² mutant larval brains compared to controls is partially suppressed.

(Kovacs et al., 2015)

Expression of th^{V85M.Scer\UAS} in wing disc clones (under the control of Scer\GAL4^tub.PU) in yki^B5 mutant animals increases clone size compared to yki^B5 alone. Co-expression of dm^Scer\UAS.cZa in this background fails to rescue the growth defect of yki^B5 mutant clones, and growth rates are similar to when th^{V85M.Scer\UAS} is expressed alone in yki^B5 mutant discs.

(Neto-Silva et al., 2010)

Suppression of rpr^GMR.PW by th^{V85M.Scer\UAS}, when driven by Scer\GAL4^GMR.PF is complete and stronger than that caused by th^Scer\UAS.cLa. Suppression of grim^GMR.PC by th^{V85M.Scer\UAS} (or th^Scer\UAS.cLa), when driven by Scer\GAL4^GMR.PF is also complete. Suppression of W^GMR.PG by th^Scer\UAS.cLa, when driven by Scer\GAL4^GMR.PF is stronger than that caused by th^{V85M.Scer\UAS}, though in both cases the suppression is incomplete.

(Lisi et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Diap1^{V85M.Scer\UAS}

Diap1^V85M.UAS

th^{V85M.Scer\UAS}

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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