FlyBase Allele Report: Dmel\Usp5[2]

General Information

Symbol

Dmel\Usp5²

Species

D. melanogaster

Name

FlyBase ID

FBal0303913

Feature type

allele

Associated gene

Dmel\Usp5

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Kovacs et al., 2015)

Mutagen

P-element activity

(Kovacs et al., 2015)

Progenitor genotype

P{EPgy2}Usp5^EY23569

(Kovacs et al., 2015)

Cytology

Description

Imprecise excision of P{EPgy2}Usp5^EY20760 has resulted in a 2175bp deletion eliminating the Usp5 gene.

(Kovacs et al., 2015)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3L:3,146,166..3,148,340 [-]

Comment:

Inferred boundaries of a 2175bp deletion resulting from the imprecise excision of P{EPgy2}Usp5^EY23569, which removes most of Usp5.

(Kovacs et al., 2015)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

chemical sensitive

(Kovacs et al., 2015)

increased cell death | larval stage

(Kovacs et al., 2015)

lethal

(Kovacs et al., 2015)

majority die during third instar larval stage

(Kovacs et al., 2015)

Phenotype Manifest In

Detailed Description

Statement

Reference

The brains of Usp5² mutant larvae contain more small rounded and pyknotic cells compared with wild type controls and exhibit a much higher level of apoptosis. Usp5²/+ heterozygotes exhibit dose-dependent sensitivity to cycloheximide relative to wild type.

(Kovacs et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Usp5² has majority die during third instar larval stage phenotype, suppressible by Scer\GAL4^da.PU/Diap1^V85M.UAS

(Kovacs et al., 2015)

Usp5² has increased cell death | larval stage phenotype, suppressible | partially by Scer\GAL4^da.PU/Diap1^V85M.UAS

(Kovacs et al., 2015)

NOT suppressed by

Statement

Reference

Usp5² has lethal phenotype, non-suppressible by Scer\GAL4^da.PU/Diap1^V85M.UAS

(Kovacs et al., 2015)

Other

Statement

Reference

Diap1^V85M.UAS, Scer\GAL4^da.PU, Usp5² has majority die during pupal stage phenotype

(Kovacs et al., 2015)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Diap1^V85M.UAS under the control of Scer\GAL4^da.PU partially suppresses the lethality and apoptosis seen in Usp5² mutants, shifting the third instar larval lethality to the pupal stage. The increased apoptotic index in Usp5² mutant larval brains compared to controls is partially suppressed.

(Kovacs et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

Usp5² is partially rescued by Scer\GAL4^da.PU/Usp5^{UAS.Tag:FLAG.cKa}

(Kovacs et al., 2015)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

CG12082²

(Kovacs et al., 2015)

DmUsp5²

(Kovacs et al., 2015)

Usp5²

(Kovacs et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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