FlyBase Allele Report: Dmel\JMJD6[FM1]

General Information

Symbol

Dmel\JMJD6^FM1

Species

D. melanogaster

Name

FlyBase ID

FBal0219088

Feature type

allele

Associated gene

Dmel\JMJD6

Associated Insertion(s)

P{?EP}JMJD6^FM1

Carried in Construct

Also Known As

PSR^FM1

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

Mutagen

Delta2-3 transposase

(Krieser et al., 2007)

Progenitor genotype

P{EP}EP561

(Krieser et al., 2007)

Associated Insertion(s)

P{?EP}JMJD6^FM1

Cytology

Description

Imprecise excision of the P{EP}EP561 insertion (located 101bp 5' of PSR), resulting in a deletion that removes the sequence encoding the first 210 amino acids of PSR. A portion of the insertion remains.

(Krieser et al., 2007)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3R:22,361,666..22,362,635 [-]

Comment:

Deletion resulting from the imprecise excision of the progentor insertion which extends from the insertion site and removes 627 bases of coding sequence from the initiator ATG. Some P-element.sequences remain.

(Krieser et al., 2007)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

enhancer of variegation, with In(1)w^m4

(Shalaby et al., 2017)

fertile

(Krieser et al., 2007)

viable

(Shalaby et al., 2017, Krieser et al., 2007)

Phenotype Manifest In

ommatidium

(Krieser et al., 2007)

Detailed Description

Statement

Reference

PSR^FM1 mutants are viable and fertile and do not display any developmental timing defects. PSR^FM1 enhances the variegated phenotype of the w locus in the In(1)w^m4 background but has no effect on the variegation in the Sb locus when combined with T(2;3)Sb^V.

(Shalaby et al., 2017)

Homozygous adults are viable and fertile with no obvious morphological defects.

Homozygous embryos show a similar number and pattern of clustered apoptotic cells (assayed using acridine orange staining) as wild-type embryos. Macrophages in homozygous embryos contain many engulfed apoptotic nuclei, as in wild-type embryos.

Mutant third larval instar eye discs show no detectable alteration in apoptosis (as detected by TUNEL staining).

At 40 hours after puparium formation (APF), pupal eye development is normal in mutants and a characteristic hexagonal lattice of interommatidial cells is seen. However, by 50 hours APF, a loss of interommatidial cells is seen, which results in ommatidial fusions. Apoptotic cells are detected by TUNEL staining at 49 hours APF in the mutant eye disc, but not in the wild-type controls.

(Krieser et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

PSR[+]/JMJD6^FM1 is an enhancer of visible phenotype of hid^GMR.PG

(Krieser et al., 2007)

PSR[+]/JMJD6^FM1 is an enhancer of visible phenotype of rpr^GMR.PW

(Krieser et al., 2007)

JMJD6^FM1/JMJD6^FM1 is an enhancer of visible phenotype of rpr^GMR.PW

(Krieser et al., 2007)

JMJD6^FM1/JMJD6^FM1 is an enhancer of visible phenotype of hid^GMR.PG

(Krieser et al., 2007)

PSR[+]/JMJD6^FM1 is an enhancer of visible phenotype of grim^GMR.PC

(Krieser et al., 2007)

JMJD6^FM1/JMJD6^FM1 is an enhancer of visible phenotype of grim^GMR.PC

(Krieser et al., 2007)

NOT Enhancer of

Statement

Reference

PSR[+]/JMJD6^FM1 is a non-enhancer of visible | adult stage phenotype of Scer\GAL4^ey.200.Exel, TAF1B^RNAi.UAS

(Shalaby et al., 2017)

PSR[+]/JMJD6^FM1 is a non-enhancer of visible | adult stage phenotype of Scer\GAL4^GMR.PF, yki^{S168A.UAS.EGFP,Tag:HA}

(Shalaby et al., 2017)

PSR[+]/JMJD6^FM1 is a non-enhancer of abnormal size | adult stage phenotype of Scer\GAL4^GMR.PF, yki^{S168A.UAS.EGFP,Tag:HA}

(Shalaby et al., 2017)

PSR[+]/JMJD6^FM1 is a non-enhancer of visible | adult stage phenotype of Scer\GAL4^C-765, smo^{5A.UAS.Tag:FLAG}

(Shalaby et al., 2017)

NOT Suppressor of

Statement

Reference

PSR[+]/JMJD6^FM1 is a non-suppressor of visible | adult stage phenotype of Scer\GAL4^ey.200.Exel, TAF1B^RNAi.UAS

(Shalaby et al., 2017)

PSR[+]/JMJD6^FM1 is a non-suppressor of visible | adult stage phenotype of Scer\GAL4^C-765, smo^{5A.UAS.Tag:FLAG}

(Shalaby et al., 2017)

PSR[+]/JMJD6^FM1 is a non-suppressor of visible | adult stage phenotype of Scer\GAL4^GMR.PF, yki^{S168A.UAS.EGFP,Tag:HA}

(Shalaby et al., 2017)

PSR[+]/JMJD6^FM1 is a non-suppressor of abnormal size | adult stage phenotype of Scer\GAL4^GMR.PF, yki^{S168A.UAS.EGFP,Tag:HA}

(Shalaby et al., 2017)

Phenotype Manifest In

Enhancer of

Statement

Reference

PSR[+]/JMJD6^FM1 is an enhancer of eye phenotype of hid^GMR.PG

(Krieser et al., 2007)

PSR[+]/JMJD6^FM1 is an enhancer of eye phenotype of rpr^GMR.PW

(Krieser et al., 2007)

JMJD6^FM1/JMJD6^FM1 is an enhancer of eye phenotype of rpr^GMR.PW

(Krieser et al., 2007)

JMJD6^FM1/JMJD6^FM1 is an enhancer of eye phenotype of hid^GMR.PG

(Krieser et al., 2007)

PSR[+]/JMJD6^FM1 is an enhancer of eye phenotype of grim^GMR.PC

(Krieser et al., 2007)

JMJD6^FM1/JMJD6^FM1 is an enhancer of eye phenotype of grim^GMR.PC

(Krieser et al., 2007)

NOT Enhancer of

Statement

Reference

PSR[+]/JMJD6^FM1 is a non-enhancer of eye phenotype of Scer\GAL4^ey.200.Exel, TAF1B^RNAi.UAS

(Shalaby et al., 2017)

PSR[+]/JMJD6^FM1 is a non-enhancer of eye phenotype of Scer\GAL4^GMR.PF, yki^{S168A.UAS.EGFP,Tag:HA}

(Shalaby et al., 2017)

PSR[+]/JMJD6^FM1 is a non-enhancer of wing vein phenotype of Scer\GAL4^C-765, smo^{5A.UAS.Tag:FLAG}

(Shalaby et al., 2017)

NOT Suppressor of

Statement

Reference

PSR[+]/JMJD6^FM1 is a non-suppressor of eye phenotype of Scer\GAL4^ey.200.Exel, TAF1B^RNAi.UAS

(Shalaby et al., 2017)

PSR[+]/JMJD6^FM1 is a non-suppressor of wing vein phenotype of Scer\GAL4^C-765, smo^{5A.UAS.Tag:FLAG}

(Shalaby et al., 2017)

PSR[+]/JMJD6^FM1 is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PF, yki^{S168A.UAS.EGFP,Tag:HA}

(Shalaby et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

Combination with a single copy of PSR^FM1 does not modify the frequency of eye tissue to antenna-like malformed structures transformation in adult flies expressing TAF1B^{dsRNA.Scer\UAS} under the control of Scer\GAL4^ey.200.Exel nor the massive eye overgrowth in flies expressing yki^{S168A.Scer\UAS.T:Avic\GFP-EGFP,T:Ivir\HA1} with Scer\GAL4^GMR.PF and while there is tendency towards worsening the disrupted wing vein phenotype of flies expressing smo^{5A.Scer\UAS.T:Zzzz\FLAG} driven by Scer\GAL4^C-765, the effect is not statistically significant.

(Shalaby et al., 2017)

The rough eye phenotype caused by rpr^GMR.PW, grim^GMR.PC or W^GMR.PG is enhanced by one copy of PSR^FM1 and is further enhanced by two copies of PSR^FM1.

(Krieser et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

605591

y¹ w^*; PBac{5HPw⁺}Ote^B279/CyO, y⁺; JMJD6^FM1/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

JMJD6^FM1

PSR^FM1

(Shalaby et al., 2018, Pinzón et al., 2017, Shalaby et al., 2017)

PSR^KO

(Pinzón et al., 2017)

dPSR^FM1

(Krieser et al., 2007)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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