FlyBase Allele Report: Dmel\Buffy[H37]

General Information

Symbol

Dmel\Buffy^H37

Species

D. melanogaster

Name

FlyBase ID

FBal0193428

Feature type

allele

Associated gene

Dmel\Buffy

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

loss of function allele

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

loss of function allele

(Sevrioukov et al., 2007)

Mutagen

Delta2-3 transposase

(Sevrioukov et al., 2007)

Progenitor genotype

P{EPgy2}Buffy^EY11259

(Sevrioukov et al., 2007)

Cytology

Description

Imprecise excision of the progenitor insertion, resulting in a 1.0kb deletion that includes almost the entire Buffy coding region.

(Sevrioukov et al., 2007)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell death

(Wu et al., 2010)

fertile

(Sevrioukov et al., 2007)

viable

(Sevrioukov et al., 2007)

Phenotype Manifest In

glial cell

(Wu et al., 2010)

Detailed Description

Statement

Reference

A significant increase in the survival of glial cells within notal microchaete clusters is seen in Buffy^H37 homozygotes.

(Wu et al., 2010)

Mutants are viable with no overt mutant adult phenotypes.

The distribution and number of apoptotic cells is similar to that of control embryos in mutant stage 11 and late stage 12 embryos.

Stage 16 mutant embryos have normal central and peripheral nervous system patterning and no fusion of commissural axon bundles is seen.

Normal histolysis of the larval midgut occurs during pupation in the mutant animals and larval salivary glands also undergo histolysis. Programmed cell death in the pupal retina also occurs normally to remove a subset of interommatidial cells. Programmed cell death in the arista is also normal in the mutants.

The pattern of mitotic cells in the developing mutant embryo is normal.

The normal random pattern of mitotic cells seen in third larval instar wing imaginal discs is not altered in Buffy^H37 mutants, and cells in these wing discs arrest normally following irradiation.

Mutant adults are not susceptible to infection by E.coli (infection by pricking with a needle dipped in a concentrated pellet of E.coli).

The apoptotic response to irradiation is slightly increased in mutant embryos compared to wild type.

(Sevrioukov et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

Buffy[+]/Buffy^H37 is a non-enhancer of visible phenotype of Hsap\BCL2L13^UAS.cNa, Scer\GAL4^GMR.PS

(Nakazawa et al., 2016)

Buffy[+], Debcl[+], Debcl^E26, Buffy^H37 is a non-enhancer of visible phenotype of Hsap\BCL2L13^UAS.cNa, Scer\GAL4^GMR.PS

(Nakazawa et al., 2016)

Buffy[+], Debcl[+], Buffy^H37, Debcl^W105 is a non-enhancer of visible phenotype of Hsap\BCL2L13^UAS.cNa, Scer\GAL4^GMR.PS

(Nakazawa et al., 2016)

Buffy[+]/Buffy^H37 is a non-enhancer of visible | adult stage phenotype of HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU

(Padash Barmchi et al., 2016)

Suppressor of

Statement

Reference

Buffy^H37 is a suppressor of increased cell death phenotype of grim^GMR.PC

(Wu et al., 2010)

NOT Suppressor of

Statement

Reference

Buffy[+]/Buffy^H37 is a non-suppressor of visible phenotype of Hsap\BCL2L13^UAS.cNa, Scer\GAL4^GMR.PS

(Nakazawa et al., 2016)

Buffy[+], Debcl[+], Debcl^E26, Buffy^H37 is a non-suppressor of visible phenotype of Hsap\BCL2L13^UAS.cNa, Scer\GAL4^GMR.PS

(Nakazawa et al., 2016)

Buffy[+], Debcl[+], Buffy^H37, Debcl^W105 is a non-suppressor of visible phenotype of Hsap\BCL2L13^UAS.cNa, Scer\GAL4^GMR.PS

(Nakazawa et al., 2016)

Other

Statement

Reference

Buffy^H37, Debcl^E26 has viable phenotype

(Sevrioukov et al., 2007)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

Buffy[+]/Buffy^H37 is a non-enhancer of eye phenotype of Hsap\BCL2L13^UAS.cNa, Scer\GAL4^GMR.PS

(Nakazawa et al., 2016)

Buffy[+], Debcl[+], Debcl^E26, Buffy^H37 is a non-enhancer of eye phenotype of Hsap\BCL2L13^UAS.cNa, Scer\GAL4^GMR.PS

(Nakazawa et al., 2016)

Buffy[+], Debcl[+], Buffy^H37, Debcl^W105 is a non-enhancer of eye phenotype of Hsap\BCL2L13^UAS.cNa, Scer\GAL4^GMR.PS

(Nakazawa et al., 2016)

Buffy[+]/Buffy^H37 is a non-enhancer of eye phenotype of HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU

(Padash Barmchi et al., 2016)

Suppressor of

Statement

Reference

Buffy^H37 is a suppressor of eye phenotype of grim^GMR.PC

(Wu et al., 2010)

Buffy^H37 is a suppressor of eye disc | third instar larval stage phenotype of grim^GMR.PC

(Wu et al., 2010)

NOT Suppressor of

Statement

Reference

Buffy[+]/Buffy^H37 is a non-suppressor of eye phenotype of Hsap\BCL2L13^UAS.cNa, Scer\GAL4^GMR.PS

(Nakazawa et al., 2016)

Buffy[+], Debcl[+], Debcl^E26, Buffy^H37 is a non-suppressor of eye phenotype of Hsap\BCL2L13^UAS.cNa, Scer\GAL4^GMR.PS

(Nakazawa et al., 2016)

Buffy[+], Debcl[+], Buffy^H37, Debcl^W105 is a non-suppressor of eye phenotype of Hsap\BCL2L13^UAS.cNa, Scer\GAL4^GMR.PS

(Nakazawa et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

The cell death seen in grim^GMR.PC eyes is abrogated in a Buffy^H37 background.

(Wu et al., 2010)

debcl^E26 Buffy^H37 double mutants are viable with no overt mutant adult phenotypes.

The distribution and number of apoptotic cells is similar to that of control embryos in debcl^E26 Buffy^H37 double mutant stage 11 and late stage 12 embryos. Stage 16 double mutant embryos have normal central and peripheral nervous system patterning and no fusion of commissural axon bundles is seen. Normal histolysis of the larval midgut occurs during pupation in the double mutant animals and larval salivary glands also undergo histolysis. Programmed cell death in the pupal retina also occurs normally to remove a subset of interommatidial cells. Programmed cell death in the arista is also normal in the double mutants.

The pattern of mitotic cells in the developing debcl^E26 Buffy^H37 double mutant embryo is normal.

debcl^E26 Buffy^H37 double adults are not susceptible to infection by E.coli (infection by pricking with a needle dipped in a concentrated pellet of E.coli).

The apoptotic response to irradiation is increased in debcl^E26 Buffy^H37 and debcl^W105 Buffy^H37 double mutant embryos compared to wild type.

(Sevrioukov et al., 2007)

Xenogenetic Interactions

Statement

Reference

The rough eye phenotype induced by expression of Hsap\BCL2L13^Scer\UAS.cNa driven by Scer\GAL4^GMR.PS is not significantly enhanced or suppressed by Debcl^E26 Buffy^H37/+, Debcl^W105 Buffy^H37/+ or Buffy^H37/+ mutations. Debcl^E26 Buffy^H37/+, Debcl^W105 Buffy^H37/+ or Buffy^H37/+ in Scer\GAL4^GMR.PS>Hsap\BCL2L13^{ΔTM.Scer\UAS} flies does not result in significant eye phenotypes.

(Nakazawa et al., 2016)

Heterozygosity for Buffy^H37 does not modify the fully penetrant rough eye phenotype resulting from the co-expression of HPV18\E6^{Scer\UAS.T:Hsap\MYC} and Hsap\UBE3A^Scer\UAS.cRa under the control of Scer\GAL4^GMR.PU, leading to severe eye necrosis.

(Padash Barmchi et al., 2016)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (3)

Bloomington

27340

w¹¹¹⁸; Buffy^H37

27338

w¹¹¹⁸; Debcl^E26 Buffy^H37