FlyBase Allele Report: Dmel\Debcl[W105]

General Information

Symbol

Dmel\Debcl^W105

Species

D. melanogaster

Name

FlyBase ID

FBal0221240

Feature type

allele

Associated gene

Dmel\Debcl

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

Mutagen

Delta2-3 transposase

(Sevrioukov et al., 2007)

Progenitor genotype

P{EPgy2}Debcl^EY05743

(Sevrioukov et al., 2007)

Cytology

Description

Imprecise excision of the progenitor insertion, resulting in a 1.2kb deletion that removes debcl sequences including the ATG codon and the first intron/exon boundary, while the promoter remains intact. A smaller than normal debcl transcript is made in the mutant, using a cryptic splice site in the first exon, which is spliced to exon 2. The first ATG in this transcript that is in the correct reading frame of debcl lies in the middle of exon 2, in the center of the conserved BH3 domain and has a good Kozak sequence upstream of it, but it is not known whether this protein is stably produced.

(Sevrioukov et al., 2007)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

The apoptotic response to irradiation is significantly decreased in mutant embryos compared to wild type.

(Sevrioukov et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

Debcl[+]/Debcl^W105 is a non-enhancer of visible phenotype of Hsap\BCL2L13^UAS.cNa, Scer\GAL4^GMR.PS

(Nakazawa et al., 2016)

Buffy[+], Debcl[+], Buffy^H37, Debcl^W105 is a non-enhancer of visible phenotype of Hsap\BCL2L13^UAS.cNa, Scer\GAL4^GMR.PS

(Nakazawa et al., 2016)

NOT Suppressor of

Statement

Reference

Debcl[+]/Debcl^W105 is a non-suppressor of visible phenotype of Hsap\BCL2L13^UAS.cNa, Scer\GAL4^GMR.PS

(Nakazawa et al., 2016)

Buffy[+], Debcl[+], Buffy^H37, Debcl^W105 is a non-suppressor of visible phenotype of Hsap\BCL2L13^UAS.cNa, Scer\GAL4^GMR.PS

(Nakazawa et al., 2016)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

Debcl[+]/Debcl^W105 is a non-enhancer of eye phenotype of Hsap\BCL2L13^UAS.cNa, Scer\GAL4^GMR.PS

(Nakazawa et al., 2016)

Buffy[+], Debcl[+], Buffy^H37, Debcl^W105 is a non-enhancer of eye phenotype of Hsap\BCL2L13^UAS.cNa, Scer\GAL4^GMR.PS

(Nakazawa et al., 2016)

NOT Suppressor of

Statement

Reference

Debcl[+]/Debcl^W105 is a non-suppressor of eye phenotype of Hsap\BCL2L13^UAS.cNa, Scer\GAL4^GMR.PS

(Nakazawa et al., 2016)

Buffy[+], Debcl[+], Buffy^H37, Debcl^W105 is a non-suppressor of eye phenotype of Hsap\BCL2L13^UAS.cNa, Scer\GAL4^GMR.PS

(Nakazawa et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

The apoptotic response to irradiation is increased in debcl^W105 Buffy^H37 double mutant embryos compared to wild type.

(Sevrioukov et al., 2007)

Xenogenetic Interactions

Statement

Reference

The rough eye phenotype induced by expression of Hsap\BCL2L13^Scer\UAS.cNa driven by Scer\GAL4^GMR.PS is not significantly enhanced or suppressed by Debcl^W105/+ or Debcl^W105 Buffy^H37/+. Debcl^W105/+ or Debcl^W105 Buffy^H37/+ does not result in significant eye phenotypes in Scer\GAL4^GMR.PS>Hsap\BCL2L13^{ΔTM.Scer\UAS} flies.

(Nakazawa et al., 2016)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

27341

w¹¹¹⁸; Debcl^W105

27339

w¹¹¹⁸; Debcl^W105 Buffy^H37

Notes on Origin

Discoverer

Comments

May be a dominant negative allele.

(Sevrioukov et al., 2007)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Debcl^W105

Drob-1^W105

(Nakazawa et al., 2016)

debcl^W105

(Lindström et al., 2017, Sevrioukov et al., 2007)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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