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Sevrioukov, E.A., Burr, J., Huang, E.W., Assi, H.H., Monserrate, J.P., Purves, D.C., Wu, J.N., Song, E.J., Brachmann, C.B. (2007). Drosophila Bcl-2 proteins participate in stress-induced apoptosis, but are not required for normal development.  genesis 45(4): 184--193.
FlyBase ID
FBrf0202360
Publication Type
Research paper
Abstract

Many developing tissues require programmed cell death (PCD) for proper formation. In mice and C. elegans, developmental PCD is regulated by the Bcl-2 family of proteins. Two bcl-2 genes are encoded in the Drosophila genome (debcl/dBorg1/Drob-1/dBok and buffy/dBorg2) and previous RNAi-based studies suggested a requirement for these in embryonic development. However, we report here that, despite the fact that many tissues in fruit flies are shaped by PCD, deletion of the bcl-2 genes does not perturb normal development. We investigated whether the fly bcl-2 genes regulate non-apoptotic processes that require caspases, but found these to be bcl-2 gene-independent. However, irradiation of the mutants demonstrates that DNA damage-induced apoptosis, mediated by Reaper, is blocked by buffy and that debcl is required to inhibit buffy. Our results demonstrate that developmental PCD regulation in the fly does not rely upon the Bcl-2 proteins, but that they provide an added layer of protection in the apoptotic response to stress.

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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    genesis
    Title
    genesis
    Publication Year
    2000-
    ISBN/ISSN
    1526-954X 1526-968X
    Data From Reference
    Aberrations (1)
    Alleles (7)
    Genes (15)
    Natural transposons (1)
    Insertions (3)
    Experimental Tools (1)
    Transgenic Constructs (2)