ark, hac-1, dapaf-1, Apaf-1, Apaf-1 related killer
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.50
None of the polypeptides share 100% sequence identity.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Dark using the Feature Mapper tool.
The longer form of Dark transcript appears to be the predominantly expressed form. It is detected in embyros, larvae, pupae, and S2 cells on northern blots. It is expressed ubiquitously in embryos and in larval eye and wing discs.
The short form of Dark transcript appears to be the minor form which is detected in embryos.
Dark transcripts are detected at all stages assayed on northern blots. Levels are elevated in times associated with histolysis of larval tissues (third instar larvae and early pupae). Levels are lowest in embryos and adults.
Dark transcripts are broadly distributed in preblastoderm embryos. By stage 7, transcripts are predominantly located in the ventral neurogenic region and around several invagination furrows. In stages 10-11, the highest Dark transcript levels are found in the ectoderm and mesoderm of the procephalic region. Later, expression is observed in a segmentally repeated pattern. Dark expression is induced by X-ray or UV irradiation.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Dark in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: Dark Ark
Gene expression is increased in response to the presence of two copies of Scer\GAL4hs.PB.
Ark mutant hemocytes are completely resistant to a Smac mimetic (which antagonises inhibitor of apoptosis proteins) or cycloheximide.
Expression of Ark in the post-embryonic central nervous system is necessary to reverse organismal lethality and to produce a viable adult.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
Loss of function mutations of Ark show reduced cell death and caspase activities in embryos.
Loss of function mutations in Ark attenuate programmed cell deaths during development, resulting in hyperplasia of the central nervous system and other abnormalities including melanotic tumours and defective wings.