FB2026_02 , released June 18, 2026
FB2026_02 , released June 18, 2026
Allele: Dmel\DarkL46
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General Information
Symbol
Dmel\DarkL46
Species
D. melanogaster
Name
FlyBase ID
FBal0191880
Feature type
allele
Associated gene
Dmel\Dark
Associated Insertion(s)
Carried in Construct
Also Known As
arkL46
Key Links
Genomic Maps

Allele class

loss of function allele

Mutagen
Nature of the Allele
Allele class

loss of function allele

(Srivastava et al., 2007, Arama et al., 2006)
Mutagen
ethyl methanesulfonate
(Srivastava et al., 2007, Arama et al., 2006)
Progenitor genotype
Cytology
Description

Amino acid replacement: C346W.

(Srivastava et al., 2007)

Amino acid replacement: W950term.

(Srivastava et al., 2007)

Amino acid replacement: C346T.

(Arama et al., 2006)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
2R:17,024,768..17,024,768 [+]
Nucleotide change:

G17024768A

Amino acid change:

W950term | Dark-PB

Reported amino acid change:

W950term

Comment:

G to A nucleotide change at the second or third position of the Trp codon leads to a nonsense mutation (exact site of mutation unspecified). Site of nucleotide substitution in mutant inferred by FlyBase base on reported amino acid change.

(Srivastava et al., 2007)
point_mutation
2R:17,022,612..17,022,612 [+]
Nucleotide change:

T17022612G

Amino acid change:

C346W | Dark-PA; C346W | Dark-PB

Reported amino acid change:

C346W

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Srivastava et al., 2007)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Salivary glands are degraded by 24 hours after puparium formation (as occurs in wild-type controls) in ArkN28/ArkL46 mutants that develop normally.

      (Berry and Baehrecke, 2007)

      ArkL46/ArkP46 flies exhibit melanotic tumors and abnormal wings.

      (Srivastava et al., 2007)

      ArkL46 mutants display severe defects during the spermatid individualization process. The cystic bulges are frequently reduced in size or appear flat due to a failure in the appropriate collection of the cytoplasm of the spermatids . The retained cytoplasm is clearly visualized as a trail along the entire length of what was supposed to have been the post-individualized portion of the spermatids. Frequently a large portion of the spermatid cytoplasm is retained in a 'mini' cystic bulge structure, which often contains part of the individualization complex. Waste bags are also reduced in size.

      (Arama et al., 2006)

      Homozygous clones in the eye result in an excess of interommatidial cells in the retina at 42 hours after puparium formation compared to wild type. Mutant clones at the edge of the eye have extra perimeter ommatidial cells that are never eliminated.

      (Mendes et al., 2006)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressor of
      Statement
      Reference

      DarkL46 is a suppressor of eye phenotype of hidGMR.PG

      (Srivastava et al., 2007)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The WGMR.PG eye ablation phenotype is strongly suppressed when eyes carry homozygous clones for ArkL46.

      (Srivastava et al., 2007)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer

      Selected as: a mutant that recessively suppresses the eye ablation phenotype caused by eye-specific overexpression of W.

      (Arama et al., 2006)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (5)
      Reported As
      Symbol Synonym
      ArkL46
      DarkL46
      (Vishal et al., 2018)
      L46
      (Srivastava et al., 2007)
      arkL46
      (Berry and Baehrecke, 2007, Srivastava et al., 2007, Mendes et al., 2006)
      darkL46
      (Suissa et al., 2011)
      Name Synonyms
      Secondary FlyBase IDs
        References (6)