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Citation
Liu, Y., Lehmann, M. (2008). A genomic response to the yeast transcription factor GAL4 in Drosophila.  Fly 2(2): 92--98.
FlyBase ID
FBrf0205590
Publication Type
Research paper
Abstract

The yeast transcription factor GAL4 is widely used in Drosophila genetics to misexpress genes that are under control of the yeast upstream activator sequence (UAS). Here we show that high levels of GAL4 change the expression of many Drosophila genes in a UAS-independent manner, including genes that encode components of important signaling pathways. We find that at least part of the genomic response to GAL4 appears to be caused by effects of GAL4 on stress and immune response pathways. Finally, using the transcription factor Senseless as an example, we demonstrate how an interaction between GAL4 and a GAL4-driven protein can impede the use of the GAL4/UAS system in experiments aimed at determining the transcriptional response to a misexpressed gene.

PubMed ID
PubMed Central ID
PMC2803330 (PMC) (EuropePMC)
DOI
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
  • FBrf0209687
Language of Publication
English
Additional Languages of Abstract
Parent Publication
Publication Type
Journal
Abbreviation
Fly
Title
Fly
Publication Year
2007-
ISBN/ISSN
1933-6934 1933-6942
Data From Reference