FlyBase Gene Report: Dmel\dos

General Information

Symbol

Dmel\dos

Species

D. melanogaster

Name

daughter of sevenless

Annotation Symbol

CG1044

Feature Type

protein_coding_gene

FlyBase ID

FBgn0016794

Gene Model Status

Current

Stock Availability

12 publicly available

Gene Summary

Essential component for signaling from various receptor tyrosine kinases such as Sevenless, TORSO and DER. Required for photoreceptor cell and wing development. (UniProt, Q9VZZ9)

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Pathway
UniProtKB
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

62E7-62E7

Sequence location

3L:2,589,414..2,595,590 [+]

Recombination map

3-3

RefSeq locus

NT_037436 REGION:2589414..2595590

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (12 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

enables protein binding

inferred from physical interaction with UniProtKB:Q08012

(Feller et al., 2002)

enables SH2 domain binding

inferred from physical interaction with FLYBASE:csw; FB:FBgn0000382

(Allard et al., 1998)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables signaling adaptor activity

inferred from biological aspect of ancestor with PANTHER:PTN005264347

(GO Reference Genome Project, 2011-)

Biological Process (7 terms)

Terms Based on Experimental Evidence (5 terms)

CV Term

Evidence

References

involved_in photoreceptor cell development

inferred from mutant phenotype

(Herbst et al., 1996)

involved_in R7 cell fate commitment

inferred from genetic interaction with FLYBASE:sev; FB:FBgn0003366

(Raabe et al., 1996)

inferred from mutant phenotype

(Herbst et al., 1996)

involved_in regulation of Ras protein signal transduction

inferred from mutant phenotype

(Feller et al., 2002, Raabe et al., 1996)

involved_in sevenless signaling pathway

inferred from genetic interaction with FLYBASE:sev; FB:FBgn0003366

(Feller et al., 2002, Raabe et al., 1996)

inferred from direct assay

(Herbst et al., 1999)

inferred from mutant phenotype

(Bausenwein et al., 2000, Herbst et al., 1996)

involved_in torso signaling pathway

inferred from genetic interaction with FLYBASE:tor; FB:FBgn0003733

(Luschnig et al., 2000)

inferred from mutant phenotype

(Raabe et al., 1996)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in anterior/posterior axis specification, embryo

traceable author statement

(Baek and Lee, 1999)

involved_in signal transduction

inferred from biological aspect of ancestor with PANTHER:PTN005264347

(GO Reference Genome Project, 2011-)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Bausenwein et al., 2000)

located_in plasma membrane

inferred from direct assay

(Raabe et al., 1996)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN005264347

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

Pathway (FlyBase)

TORSO SIGNALING PATHWAY CORE COMPONENTS

SEVENLESS SIGNALING PATHWAY CORE COMPONENTS

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Pathway (FlyBase)

TORSO SIGNALING PATHWAY CORE COMPONENTS -: The formation of Drosophila embryonic termini is controlled by the localized activation of Torso (tor) receptor tyrosine kinase. The Torso signaling pathway acts via the canonical Ras/Raf/MAP kinase cascade. (Adapted from FBrf0157176.)
SEVENLESS SIGNALING PATHWAY CORE COMPONENTS -: The specification of the R7 photoreceptor cell in each ommatidium of the developing Drosophila eye is dependent on activation of Sevenless receptor tyrosine kinase, which acts via the canonical Ras/Raf/MAP kinase cascade to promote the expression of lz and pros. sev, expressed in presumptive R7 cells, is activated by binding to Bride of Sevenless (boss), a seven-transmembrane protein expressed in R8 cells. (Adapted from FBrf0127283 and FBrf0221727).

Protein Function (UniProtKB)

Essential component for signaling from various receptor tyrosine kinases such as Sevenless, TORSO and DER. Required for photoreceptor cell and wing development.

(UniProt, Q9VZZ9)

Summary (Interactive Fly)

an adaptor protein that facilitates signal transduction as a downstream component of the receptor tyrosine kinases Sevenless, Egf receptor and Torso

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\dos for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VZZ9)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.45

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

dos-RA

FBtr0072926

NM_079166

3254

878

dos-RB

FBtr0072927

NM_167956

4666

798

Additional Transcript Data and Comments

Reported size (kB)

3.2 (northern blot)

(Raabe et al., 1996)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

dos-PA

FBpp0072803

95.5

878

7.47

FBpp0072804

85.6

798

6.80

NP_728757

AAF47663

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

878 (aa); 115 (kD observed); 96 (kD predicted)

(Raabe et al., 1996)

Comments

External Data

Subunit Structure (UniProtKB)

Interacts with DRK.

(UniProt, Q9VZZ9)

Post Translational Modification

Phosphorylated on Tyr-801 and Tyr-854 in response to sevenless activation, which initiates the recruitment of the phosphatase CSW.

(UniProt, Q9VZZ9)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q9VZZ9

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\dos using the Feature Mapper tool.

External Data

Crossreferences

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.04

Transcript Expression

Additional Descriptive Data

dos transcripts are expressed predominantly in early embryos, late larvae, and early pupae.

(Raabe et al., 1996)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

eye disc morphogenetic furrow

(Raabe et al., 1996)

eye-antennal disc

(Raabe et al., 1996)

eye disc posterior to the morphogenetic furrow

(Raabe et al., 1996)

Additional Descriptive Data

dos protein is found at low levels in all cells of the eye imaginal disc. It is detected at higher levels in the membranes of cells in the morphogenetic furrow and in clusters of cells behind the furrow

(Raabe et al., 1996)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Bausenwein et al., 2000)

located_in plasma membrane

inferred from direct assay

(Raabe et al., 1996)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\dos in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\dos

View exonic expression by tissue for Dmel\dos

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0016794

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

38321/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0016794

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0016794

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0016794

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG1044

Flygut - An atlas of the Drosophila adult midgut

FBgn0016794

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 14 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 31 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of dos

Transgenic constructs containing regulatory region of dos

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 2 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal | recessive

partially lethal - majority die (with dos^1.46)

dos^2.42

partially lethal - majority die (with dos^2.42)

dos^1.46

viable

dos^EY03093

Sterility

Allele

fertile

dos^EY03093

Other Phenotypes

Allele

some die during larval stage (with dos^1.46)

dos^2.42

some die during larval stage (with dos^2.42)

dos^1.46

wild-type

Phenotype manifest in

Allele

anterior crossvein, with dos^P115/dos^R31

dos^{R644K.R696K.hs.2sev}

anterior crossvein (with dos^P115), with dos^{R644K.R696K.hs.2sev}

dos^R31

anterior crossvein (with dos^R31), with dos^{R644K.R696K.hs.2sev}

dos^P115

cephalopharyngeal skeleton | germline clone

dos^P115

cone cell

dos^R31

embryonic abdominal segment 8 | germline clone

embryonic head | germline clone

eye

eye, with dos^P115

dos^{R644K.R696K.hs.2sev}

eye, with dos^P115/dos^R31

dos^{R644K.R696K.hs.2sev}

eye, with dos^R31

dos^{R696K.hs.2sev}

eye, with dos^{R644K.R696K.hs.2sev}

dos^P115

eye, with dos^{R696K.hs.2sev}

dos^R31

eye (with dos^P115), with dos^{R644K.R696K.hs.2sev}

dos^R31

eye (with dos^R31), with dos^{R644K.R696K.hs.2sev}

dos^P115

eye | somatic clone

eye photoreceptor cell

eye photoreceptor cell | somatic clone

dos^R31

filzkorper | embryonic stage | germline clone

filzkorper | germline clone

dos^P115

glial cell, with Scer\GAL4^repo, Scer\GAL80^ts.αTub84B

Malpighian tubule | germline clone

dos^P115

muscle cell of ventral acute muscle 2 | precursor

dos^R31

ommatidium

photoreceptor

dos^1.46

photoreceptor | somatic clone

dos^1.46

photoreceptor cell R7

photoreceptor cell R7, with dos^P115

dos^{R644K.R696K.hs.2sev}

photoreceptor cell R7, with dos^P115/dos^R31

dos^{R644K.R696K.hs.2sev}

photoreceptor cell R7, with dos^R31

dos^{R696K.hs.2sev}

photoreceptor cell R7, with dos^{R644K.R696K.hs.2sev}

dos^P115

photoreceptor cell R7, with dos^{R696K.hs.2sev}

dos^R31

photoreceptor cell R7 (with dos^P115), with dos^{R644K.R696K.hs.2sev}

dos^R31

photoreceptor cell R7 (with dos^R31), with dos^{R644K.R696K.hs.2sev}

dos^P115

vesicle, with Scer\GAL4^repo, Scer\GAL80^ts.αTub84B

wing vein

wing vein, with dos^P115

dos^{R644K.R696K.hs.2sev}

wing vein, with dos^P115/dos^R31

dos^{R644K.R696K.hs.2sev}

wing vein, with dos^R31

dos^{R696K.hs.2sev}

wing vein, with dos^{R644K.R696K.hs.2sev}

dos^P115

wing vein, with dos^{R696K.hs.2sev}

dos^R31

wing vein (with dos^P115), with dos^{R644K.R696K.hs.2sev}

dos^R31

wing vein (with dos^R31), with dos^{R644K.R696K.hs.2sev}

dos^P115

wing vein L4, with dos^P115/dos^R31

dos^{R644K.R696K.hs.2sev}

wing vein L4 (with dos^P115), with dos^{R644K.R696K.hs.2sev}

dos^R31

wing vein L4 (with dos^R31), with dos^{R644K.R696K.hs.2sev}

dos^P115

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (13)

Hsap\GAB1

7 of 14

Yes

Hsap\GAB4

7 of 14

Yes

Hsap\GAB2

6 of 14

Yes

Hsap\GAB3

6 of 14

Yes

Hsap\APBB1IP

1 of 14

Hsap\COBLL1

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\RAPH1

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (9)

Rnor\Gab1

7 of 14

Yes

Rnor\Gab2

6 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\Raph1

1 of 14

Mus musculus (laboratory mouse) (13)

Mmus\Gab1

8 of 14

Yes

Mmus\Gab2

7 of 14

Yes

Mmus\Gab3

7 of 14

Yes

Mmus\Apbb1ip

1 of 14

Mmus\Cobll1

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Raph1

1 of 14

Xenopus tropicalis (Western clawed frog) (9)

Xtro\gab2

5 of 13

Yes

Xtro\gab3

5 of 13

Yes

Xtro\gab1

4 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (17)

Drer\gab1

8 of 14

Yes

Drer\gab2

7 of 14

Yes

Drer\apbb1ip

1 of 14

Drer\cobll1a

1 of 14

Yes

Drer\gab3

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\plekhs1.2

1 of 14

Yes

Drer\plekhs1.3

1 of 14

Yes

Drer\plekhs1.4

1 of 14

Yes

Drer\raph1a

1 of 14

Drer\raph1b

1 of 14

Drer\si:ch211-234p6.5

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (2)

Cele\soc-1

2 of 14

Yes

Cele\Y37D8A.4

1 of 14

Anopheles gambiae (African malaria mosquito) (3)

Agam\AgaP_AGAP005826

12 of 12

Yes

Agam\AgaP_AGAP002695

1 of 12

Agam\AgaP_AGAP003638

1 of 12

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:dos. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (2)

CG2841

1 of 13

pico

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

GAB1; GRB2 associated binding protein 1

model of autosomal recessive nonsyndromic deafness 26

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

GAB1; GRB2 associated binding protein 1

7 of 14

604439

DEAFNESS, AUTOSOMAL RECESSIVE 26; DFNB26

autosomal recessive nonsyndromic deafness 26

GAB4; GRB2 associated binding protein family member 4

7 of 14

GAB2; GRB2 associated binding protein 2

6 of 14

606203

GAB3; GRB2 associated binding protein 3

6 of 14

300482

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

dos - 14-3-3ζ

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

dos - Acn

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

dos - Actn

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

dos - Akap200

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

dos - Brr2

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

dos - CG11417

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

dos - CG6686

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

dos - CG7668

experimental knowledge based

(Guruharsha et al., 2011)

dos - CanA-14F

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

dos - Ccs

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

dos - Flo2

experimental knowledge based

(Guruharsha et al., 2011)

dos - HSPC300

experimental knowledge based

(Guruharsha et al., 2011)

dos - Hsc70-1

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

dos - Hsp83

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

dos - Ist1

experimental knowledge based

(Guruharsha et al., 2011)

dos - Mbs

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

dos - Mppa

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

dos - Nup50

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

dos - PH4αEFB

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

dos - RAF2

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

dos - Rbpn-5

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

dos - Rip11

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

dos - RpL17

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

dos - Saf-B

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

dos - Shc

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

dos - Syb

experimental knowledge based

(Guruharsha et al., 2011)

dos - UQCR-C1

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

dos - Vps60

experimental knowledge based

(Guruharsha et al., 2011)

dos - Ythdf

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

dos - ana2

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

dos - caz

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

dos - cher

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

dos - chico

experimental knowledge based

(Guruharsha et al., 2011)

dos - cindr

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

dos - cmb

experimental knowledge based

(Guruharsha et al., 2011)

dos - cpa

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

dos - cpb

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

dos - csw

anti tag coimmunoprecipitation, peptide massfingerprinting, pull down, western blot

(Friedman et al., 2011, Allard et al., 1998)

dos - drk

nuclear magnetic resonance, pull down, western blot, anti tag coimmunoprecipitation, peptide massfingerprinting, anti tag western blot

(Sayeesh et al., 2023, Sayeesh et al., 2022, Friedman et al., 2011, Feller et al., 2002)

dos - eIF3f1

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

dos - glo

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

dos - lds

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

dos - mib2

experimental knowledge based

(Guruharsha et al., 2011)

dos - nonA-l

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

dos - pins

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

dos - qkr58E-2

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

dos - rin

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

dos - sesB

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

dos - vlc

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

dos

suppressible

csw

(Johnson Hamlet and Perkins, 2001)

dos|sev

enhanceable

dos::Hsap\GAB1

(Bausenwein et al., 2000)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

ato

suppressible

dos

(White and Jarman, 2000)

csw

enhanceable

dos

(Firth et al., 2000, Herbst et al., 1999, Herbst et al., 1996)

csw

suppressible

dos

(Herbst et al., 1999, Herbst et al., 1996)

sev

suppressible

dos

(Feller et al., 2002, Bausenwein et al., 2000, Herbst et al., 1999, Herbst et al., 1996, Raabe et al., 1996)

sev

enhanceable

dos

(Herbst et al., 1999)

enhanceable

dos

(Murillo-Maldonado et al., 2011)

suppressible

dos

(Murillo-Maldonado et al., 2011)

tor

suppressible

dos

(Luschnig et al., 2000)

External Data

Subunit Structure (UniProtKB)

Interacts with DRK.

(UniProt, Q9VZZ9 )

Linkouts

BioGRID - A database of protein and genetic interactions.

63826

DroID - A comprehensive database of gene and protein interactions.

FBgn0016794

MIST (genetic) - An integrated Molecular Interaction Database

38321

MIST (protein-protein) - An integrated Molecular Interaction Database

38321

Pathways

Signaling Pathways (FlyBase)

TORSO SIGNALING PATHWAY CORE COMPONENTS
SEVENLESS SIGNALING PATHWAY CORE COMPONENTS

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

MAPK signaling pathway - fly

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q9VZZ9

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-3

Cytogenetic map

62E7-62E7

Sequence location

3L:2,589,414..2,595,590 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

62E7-62E7

Limits computationally determined from genome sequence between P{PZ}Spn⁰⁶⁹¹¹ and P{PZ}l(3)06803⁰⁶⁸⁰³

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-3

(FlyBase, 2016)

3-5.0

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (12)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

85196

w¹¹¹⁸; PBac{WH}dos^f01808

31766

y¹ v¹; P{TRiP.HM04078}attP2

15731

y¹ w^67c23; P{EPgy2}dos^EY04266

19901

y¹ w^67c23; P{EPgy2}dos^EY03093

80716

y¹ sc^* v¹ sev²¹; P{TOE.GS01201}attP40

6845

w^*; P{lacW}dos^P115 Diap1^th-1 cu¹ sr¹/TM6B, Tb¹

Korea

11558

P{EP}dos^G14165

Kyoto

112124

y^* w^*; P{GawB}dos^NP0278 / TM6, P{UAS-lacZ.UW23-1}UW23-1

1044R-2

1044R-3

v330182

v101358

Genomic Clones (19)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (59)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

DPiM_SD02517

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0016794

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0016794

Antibody Information

Laboratory Generated Antibodies

monoclonal

(Raabe et al., 1996)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Gene expression is increased in response to the presence of two copies of Scer\GAL4^hs.PB.

(Liu and Lehmann, 2008)

RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a greater than three-fold increase in AttA activity in response to heat-killed E.coli after ecdysone treatment in S2 cells.

(Kleino et al., 2005)

Both drk binding sites present in dos have an important function in the context of sev and Egfr mediated signalling processes.

(Feller et al., 2002)

Phosphorylation of residues 801 and 854 of dos is required for the recruitment of csw. This appears to be a primary function of for dos.

(Herbst et al., 1999)

The interaction between csw and dos proteins is dependent on csw SH2 domain function.

(Allard et al., 1998)

The protein encoded by dos is a csw substrate and may also be a direct or indirect target for the tyrosine kinase activity of sev. Mutations that inactivate dos dominantly enhance the effects of reduced csw activity and suppress the effects of either excessive sev or csw activity. Results indicate that dos is a crucial component of the sev signalling pathway and strongly suggest that dephosphorylation of dos by csw promotes R7 photoreceptor differentiation.

(Herbst et al., 1996)

dos functions upstream or independent of Ras85D and defines a signalling pathway that is independent of direct binding of the drk SH2/SH3 adaptor protein to the sev receptor tyrosine kinase.

(Raabe et al., 1996)

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: dos CG1044

(Liang, 1999.11)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (16)

Reported As

Symbol Synonym

141511_at

(Kristensen et al., 2005)

CG1044

(Meadows, 2017.1.11, Keleman et al., 2009.8.5, Ni et al., 2008)

DOS

(Davidson and Wood, 2020, Freeman, 2015, Slack et al., 2010, Botham et al., 2008, Borland et al., 2001, Johnson Hamlet and Perkins, 2001, Roche, 2000, Herbst et al., 1999)

Dos

(Sayeesh et al., 2023, Sayeesh et al., 2022, Harnish et al., 2021, Bier and Guichard, 2012, Friedman et al., 2011, Murillo-Maldonado et al., 2011, Hilger et al., 2009, Duchek et al., 2001, Kockel et al., 2001, Li et al., 2000, Baek and Lee, 1999, MacDougall and Waterfield, 1996, Raabe, 1996.4.22)

E(csw)3A

(Kumar and Moses, 2000)

E(csw^CS)3A

(Herbst et al., 1996)

EC3-4

(Baril et al., 2014)

Gab-1

(Settle et al., 2003)

Su(sev)3A

(Kumar and Moses, 2000)

Su(sev^S11)3A

(Raabe et al., 1996)

Name Synonyms

Daughter of Sevenless

(Botham et al., 2008, Wandler et al., 2008)

Daughter of sevenless

(Murillo-Maldonado et al., 2011)

Enhancer of corkscrew-CS

(Herbst et al., 1996)

Suppressor of sevenless-S11

(Raabe et al., 1996)

daughter of sevenless

(Ashton-Beaucage and Therrien, 2017, Young and Marcotte, 2017, Lu et al., 2014, Oishi et al., 2006)

Secondary FlyBase IDs

Datasets (1)

Study focus (1)

Experimental Role

Project

Project Type

Title

bait_protein

RTK-Ras-ERK_interaction_map

interactome

Interaction map generated by purification of receptor tyrosine kinase pathway factors, with identification of copurifying proteins by mass spectrometry.

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 58 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

38321

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VZZ9

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9VZZ9

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VZZ9

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0016794

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

38321/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0016794

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0016794

FlyMine - An integrated database for Drosophila genomics

FBgn0016794

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG1044

MARRVEL_MODEL - MARRVEL (model organism gene)

38321

Linkouts

BioGRID - A database of protein and genetic interactions.

63826

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0016794

DroID - A comprehensive database of gene and protein interactions.

FBgn0016794

DRSC - Results frm RNAi screens

FBgn0016794

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0016794

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0016794

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG1044

Flygut - An atlas of the Drosophila adult midgut

FBgn0016794

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0016794

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC008021

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

MAPK signaling pathway - fly

MIST (genetic) - An integrated Molecular Interaction Database

38321

MIST (protein-protein) - An integrated Molecular Interaction Database

38321

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q9VZZ9

References (110)

The following external sites may use different assemblies or annotations than FlyBase.

-0.04

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