FlyBase Allele Report: Dmel\dos[R644K.R696K.hs.2sev]

General Information

Symbol

Dmel\dos^{R644K.R696K.hs.2sev}

Species

D. melanogaster

Name

FlyBase ID

FBal0138278

Feature type

allele

Associated gene

Dmel\dos

Associated Insertion(s)

Carried in Construct

P{hsp-dos.R644K.R696K}

Key Links

Transgenic product class

missense_variant

(Feller et al., 2002)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Feller et al., 2002)

Mutagen

in vitro construct

(Feller et al., 2002)

Progenitor genotype

Carried in construct

P{hsp-dos.R644K.R696K}

Cytology

Description

A duplicated sev enhancer and the Hsp70 promoter drive expression of a mutated dos cDNA that carries amino acid replacements R644K and R696K.

(Feller et al., 2002)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

sev

Encoded product / tool

dos

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

anterior crossvein, with dos^P115/dos^R31

(Feller et al., 2002)

eye, with dos^P115

(Feller et al., 2002)

eye, with dos^P115/dos^R31

(Feller et al., 2002)

photoreceptor cell R7, with dos^P115

(Feller et al., 2002)

photoreceptor cell R7, with dos^P115/dos^R31

(Feller et al., 2002)

wing vein, with dos^P115

(Feller et al., 2002)

wing vein, with dos^P115/dos^R31

(Feller et al., 2002)

wing vein L4, with dos^P115/dos^R31

(Feller et al., 2002)

Detailed Description

Statement

Reference

The addition of dos^{R644K.R696K.hs.2sev} to dos^R31/dos^P115 or dos^P115 homozygous animals results in a few flies having greatly reduced eyes with only a few photoreceptors present in most ommatidia. Wing vein formation is also disrupted in the case of dos^{R644K.R696K.hs.2sev}, dos^R31/dos^P115 flies, the L4 vein does not reach the wing margin and the anterior cross vein is completely missing.

(Feller et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

The addition of both dos^R31 and dos^{R644K.R696K.hs.2sev} continues to suppress the rough eye phenotype.

(Feller et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

dos^{R644K.R696K.hs.2sev} partially rescues dos^P115/dos^R31

(Feller et al., 2002)

Fails to rescue

dos^{R644K.R696K.hs.2sev} fails to rescue dos^R31

(Feller et al., 2002)

Comments

While the addition of dos^{R644K.R696K.hs.2sev} to dos^R31 homozygotes results in no rescue, its addition to dos^R31/dos^P115 or dos^P115 homozygous animals results in a few flies having greatly reduced eyes with only a few photoreceptors present in most ommatidia. Wing vein formation is also disrupted.

(Feller et al., 2002)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

dos^{R644K.R696K.hs.2sev}

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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