FlyBase Allele Report: Dmel\dos[R31]

General Information

Symbol

Dmel\dos^R31

Species

D. melanogaster

Name

FlyBase ID

FBal0051662

Feature type

allele

Associated gene

Dmel\dos

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Cytology

Description

Amino acid replacement: ?463term.

(Bausenwein et al., 2000)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:2,593,677..2,593,677 [+]

Nucleotide change:

A2593677T

Amino acid change:

K463term | dos-PA; K383term | dos-PB

Reported amino acid change:

?463term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change

(Bausenwein et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | recessive

(Feller et al., 2002, Raabe et al., 1996)

Phenotype Manifest In

anterior crossvein (with dos^P115), with dos^{R644K.R696K.hs.2sev}

(Feller et al., 2002)

cone cell

(Feller et al., 2002)

embryonic abdominal segment 8 | germline clone

(Raabe et al., 1996)

embryonic head | germline clone

(Raabe et al., 1996)

eye

(Feller et al., 2002)

eye, with dos^{R696K.hs.2sev}

(Feller et al., 2002)

eye (with dos^P115), with dos^{R644K.R696K.hs.2sev}

(Feller et al., 2002)

eye photoreceptor cell

(Raabe et al., 1996)

eye photoreceptor cell | somatic clone

(Bausenwein et al., 2000)

filzkorper | embryonic stage | germline clone

(Raabe et al., 1996)

muscle cell of ventral acute muscle 2 | precursor

(Johnson Hamlet and Perkins, 2001)

photoreceptor cell R7

(Feller et al., 2002)

photoreceptor cell R7, with dos^{R696K.hs.2sev}

(Feller et al., 2002)

photoreceptor cell R7 (with dos^P115), with dos^{R644K.R696K.hs.2sev}

(Feller et al., 2002)

wing vein

(Raabe et al., 1996)

wing vein, with dos^{R696K.hs.2sev}

(Feller et al., 2002)

wing vein (with dos^P115), with dos^{R644K.R696K.hs.2sev}

(Feller et al., 2002)

wing vein L4 (with dos^P115), with dos^{R644K.R696K.hs.2sev}

(Feller et al., 2002)

Detailed Description

Statement

Reference

When mutant somatic clones are made in the border cells no effect is seen.

(Duchek et al., 2001)

Homozygous embryos show loss of VA2 muscle precursor cells (25% of hemisegments contain VA2 precursor cells).

(Johnson Hamlet and Perkins, 2001)

Induction of somatic clones in the developing eye results in the frequent absence of photoreceptor cells of different identities.

(Bausenwein et al., 2000)

Able to induce R7 cell development at a reduced frequency. dos^R31/dos^P115 transheterozygotes are lethal. Repeated heat shock induction of P{sE-dos} during development is sufficient to rescue the lethality of dos^R31 and dos^P115 homozygotes and dos^R31/dos^P115 transheterozygotes. Mitotic clones in the eye rarely differentiate photoreceptor cells. In the wing clonal cells are unable to differentiate vein structures. Embryos derived from germline clones exhibit very distinct defects in the terminal structures, they rarely differentiate filzkorper and the abdominal segment A8 is frequently affected and the head skeleton is reduced in size.

(Raabe et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

dos[+]/dos^R31 is an enhancer of abnormal size | adult stage phenotype of sl²

(Murillo-Maldonado et al., 2011)

Suppressor of

Statement

Reference

dos[+]/dos^R31 is a suppressor of increased cell number | adult stage phenotype of sl²

(Murillo-Maldonado et al., 2011)

Phenotype Manifest In

Enhanced by

Statement

Reference

dos^R31, sev^S11.Tag:MYC has photoreceptor cell R7 phenotype, enhanceable by dos::Hsap\GAB1^hs.2sev.GFP

(Bausenwein et al., 2000)

Suppressed by

Statement

Reference

dos^R31 has muscle cell of ventral acute muscle 2 | precursor phenotype, suppressible by Scer\GAL4^twi.PG/csw^{UAS.Tag:Myr(Src64B)}

(Johnson Hamlet and Perkins, 2001)

Enhancer of

Statement

Reference

dos[+]/dos^R31 is an enhancer of wing blade phenotype of sl²

(Murillo-Maldonado et al., 2011)

dos^R31 is an enhancer of phenotype of csw^lf

(Firth et al., 2000)

Suppressor of

Statement

Reference

dos[+]/dos^R31 is a suppressor of wing vein | ectopic phenotype of sl²

(Murillo-Maldonado et al., 2011)

dos[+]/dos^R31 is a suppressor of photoreceptor cell R7 | adult stage phenotype of sl²

(Murillo-Maldonado et al., 2011)

dos^R31 is a suppressor of cone cell phenotype of sev^S11.Tag:MYC

(Feller et al., 2002)

dos^R31 is a suppressor of photoreceptor cell R7 | ectopic phenotype of sev^S11.Tag:MYC

(Feller et al., 2002)

dos^R31 is a suppressor of eye phenotype of sev^S11.Tag:MYC

(Feller et al., 2002)

dos^R31 is a suppressor of photoreceptor cell R7 phenotype of sev^S11.Tag:MYC

(Bausenwein et al., 2000)

dos^R31 is a suppressor of phenotype of sev^S11.Tag:MYC

(Raabe et al., 1996)

Additional Comments

Genetic Interactions

Statement

Reference

One copy of dos^R31 enhances the reduction in wing blade area seen in homozygous sl² males.

One copy of dos^R31 partially suppresses the ectopic wing vein phenotype seen in sl² homozygotes.

One copy of dos^R31 partially suppresses the percentage of sl² mutant ommatidia that contain extra R7 photoreceptors.

(Murillo-Maldonado et al., 2011)

The addition of dos^R31 almost completely suppresses the rough eye phenotype seen in sev^{S11.T:Hsap\MYC} animals. The addition of both dos^R31 and dos^hs.2sev does not suppress the phenotype. The addition of both dos^R31 and dos^{R644K.R696K.hs.2sev} or dos^{R696K.hs.2sev} continues to suppress the rough eye phenotype. The addition of both dos^R31 and dos^{R644K.hs.2sev} partially the rough eye phenotype.

(Feller et al., 2002)

Expression of csw::Src64B^{src90.Scer\UAS} under the control of Scer\GAL4^twi.PG significantly suppresses the loss of VA2 muscle precursor cells seen in dos^R31 embryos (41% of hemisegments have VA2 cells).

(Johnson Hamlet and Perkins, 2001)

The addition of heterozygous dos^R31 suppresses the rough eye phenotype seen in sev^{S11.T:Hsap\MYC} flies.

(Bausenwein et al., 2000)

Fails to suppress the mutant eye phenotype of Ras85D^V12. Removal of one copy of dos from sev::tor^{13D:Y2546F.hs.sev} individuals causes a significant reduction in ommatidia with multiple R7 cells.

(Raabe et al., 1996)

Xenogenetic Interactions

Statement

Reference

The addition of dos::Hsap\GAB1^{hs.T:Avic\GFP} to dos^R31 homozygotes or dos^P115/dos^R31 flies leads to the recovery of fully viable flies.

(Bausenwein et al., 2000)

Complementation and Rescue Data

Rescued by

dos^R31 is rescued by dos^hs.2sev

(Feller et al., 2002)

dos^R31 is rescued by dos^Y801F.mBa

(Bausenwein et al., 2000)

dos^R31 is rescued by dos^{Y358F.Y537F.mBa}

(Bausenwein et al., 2000)

dos^R31 is rescued by dos^hs.2sev.GFP

(Bausenwein et al., 2000)

dos^R31 is rescued by dos^{Y631F.hs.2sev}

(Bausenwein et al., 2000)

dos^R31 is rescued by dos^{Y316F.hs.2sev}

(Bausenwein et al., 2000)

dos^R31 is rescued by dos^Y537F.mBa

(Bausenwein et al., 2000)

dos^R31 is rescued by dos^{Y358F.Y537F.hs.2sev}

(Bausenwein et al., 2000)

dos^R31 is rescued by dos^+mBa

(Bausenwein et al., 2000)

dos^R31 is rescued by dos^Y631F.mBa

(Bausenwein et al., 2000)

dos^R31 is rescued by dos^Y316F.mBa

(Bausenwein et al., 2000)

Partially rescued by

dos^P115/dos^R31 is partially rescued by dos^{R644K.hs.2sev}

(Feller et al., 2002)

dos^P115/dos^R31 is partially rescued by dos^{R644K.R696K.hs.2sev}

(Feller et al., 2002)

dos^P115/dos^R31 is partially rescued by dos^{R696K.hs.2sev}

(Feller et al., 2002)

dos^R31 is partially rescued by dos^{R696K.hs.2sev}

(Feller et al., 2002)

dos^R31 is partially rescued by dos^{R644K.hs.2sev}

(Feller et al., 2002)

Not rescued by

dos^R31 is not rescued by dos^{R644K.R696K.hs.2sev}

(Feller et al., 2002)

dos^R31 is not rescued by dos^{Y801F.hs.2sev}

(Bausenwein et al., 2000)

dos^R31 is not rescued by dos^{ΔPH.hs.2sev.GFP}

(Bausenwein et al., 2000)

dos^R31 is not rescued by dos^W104A.mBa

(Bausenwein et al., 2000)

dos^R31 is not rescued by dos^{W104A.hs.2sev.GFP}

(Bausenwein et al., 2000)

Comments

The addition of dos^{hs.T:Avic\GFP} to dos^R31 homozygotes or dos^P115/dos^R31 flies leads to the recovery of fully viable flies. The addition of dos^{W104A.hs.T:Avic\GFP} or dos^{ΔPH.hs.T:Avic\GFP} to dos^R31 homozygotes or dos^P115/dos^R31 flies fails to produce viable flies. A single copy of dos^Y316F.hs rescues the eye phenotype seen in the eye when dos^R31 somatic clones are induced. The addition of dos^W104A.mBa fails to rescue this phenotype. The addition of dos^{hs.T:Avic\GFP} (but not dos^{ΔPH.hs.T:Avic\GFP}) to dos^R31/sev^{S11.T:Hsap\MYC} flies restores the multiple R7 phenotype seen in dos^{ΔPH.hs.T:Avic\GFP}/sev^{S11.T:Hsap\MYC} flies.

(Bausenwein et al., 2000)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

DOS^R31

(Johnson Hamlet and Perkins, 2001)

Dos^R31

(Murillo-Maldonado et al., 2011, Duchek et al., 2001, Li et al., 2000)

dos^R31

Name Synonyms

Secondary FlyBase IDs

References (9)

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