FlyBase Allele Report: Dmel\dos[hs.2sev.GFP]

General Information

Symbol

Dmel\dos^hs.2sev.GFP

Species

D. melanogaster

Name

FlyBase ID

FBal0104641

Feature type

allele

Associated gene

Dmel\dos

Associated Insertion(s)

Carried in Construct

P{hs-dos-GFP}

Key Links

Transgenic product class

cDNA

(Bausenwein et al., 2000)

wild_type

(Bausenwein et al., 2000)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Bausenwein et al., 2000)

wild_type

(Bausenwein et al., 2000)

Mutagen

in vitro construct

(Bausenwein et al., 2000)

Progenitor genotype

Carried in construct

P{hs-dos-GFP}

Cytology

Description

A duplicated sev enhancer and Hsp70 promoter drive expression of a dos cDNA. The coding sequence is tagged at the C-terminal end with GFP.

(Bausenwein et al., 2000)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

sev

Encoded product / tool

dos

Tagged with

GFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

wild-type

(Bausenwein et al., 2000)

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

dos^hs.2sev.GFP rescues dos^P115

(Bausenwein et al., 2000)

dos^hs.2sev.GFP rescues dos^R31

(Bausenwein et al., 2000)

Comments

The addition of dos^{hs.T:Avic\GFP} (but not dos^{ΔPH.hs.T:Avic\GFP}) to dos^R31/sev^{S11.T:Hsap\MYC} flies restores the multiple R7 phenotype suppressed in sev^{S11.T:Hsap\MYC} flies when dos^R31 is added. A single copy of dos^{hs.T:Avic\GFP} rescues the lethality of homozygous dos^R31, dos^P115 or dos^P115/dos^R31 transheterozygotes.

(Bausenwein et al., 2000)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

dos^hs.2sev.GFP

dos^hs.GFP

dos^{hs.T:Avic\GFP}

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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