FlyBase Allele Report: Dmel\csw[UAS.Tag:Myr(Src64B)]

General Information

Symbol

Dmel\csw^{UAS.Tag:Myr(Src64B)}

Species

D. melanogaster

Name

FlyBase ID

FBal0086670

Feature type

allele

Associated gene

Dmel\csw

Associated Insertion(s)

Carried in Construct

P{UAS-csw.src90}

Also Known As

UAS-csw^src90

Key Links

Transgenic product class

gain_of_function_variant

(Johnson Hamlet and Perkins, 2001)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

gain_of_function_variant

(Johnson Hamlet and Perkins, 2001)

Mutagen

in vitro construct

(Johnson Hamlet and Perkins, 2001, Johnson and Perkins, 1998)

Progenitor genotype

Carried in construct

P{UAS-csw.src90}

Cytology

Description

UASt regulatory sequences drive expression of a wild-type csw coding region with a Tag:Myr(Src64B) myristoylation sequence fused to its N-terminus. The myristoylation signal results in the protein being constitutively targetted to the plasma membrane, producing a gain-of-function protein.

(Johnson Hamlet and Perkins, 2001)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

csw

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^71B

(Johnson Hamlet and Perkins, 2001)

Phenotype Manifest In

dorsal appendage, with Scer\GAL4^CY2

(Johnson Hamlet and Perkins, 2001)

dorsal vessel wall cell | embryonic stage, with Scer\GAL4^unspecified

(Johnson and Perkins, 1998)

ectoderm | ventral, with Scer\GAL4^unspecified

(Johnson and Perkins, 1998)

egg chorion, with Scer\GAL4^CY2

(Johnson Hamlet and Perkins, 2001)

embryo, with Scer\GAL4^55B

(Johnson Hamlet and Perkins, 2001)

embryonic myoblast, with Scer\GAL4^unspecified

(Johnson and Perkins, 1998)

larval tracheal system, with Scer\GAL4^btl.PS

(Jarvis et al., 2006)

larval tracheocyte | ectopic | embryonic stage, with Scer\GAL4^unspecified

(Johnson and Perkins, 1998)

mesoderm, with Scer\GAL4^twi.PG

(Johnson Hamlet and Perkins, 2001)

terminal tracheal cell | increased number, with Scer\GAL4^btl.PS

(Jarvis et al., 2006)

tracheal branch primordium, with Scer\GAL4^unspecified

(Johnson and Perkins, 1998)

wing vein | ectopic, with Scer\GAL4^71B

(Johnson Hamlet and Perkins, 2001)

Detailed Description

Statement

Reference

Expression of csw::Src64B^{src90.Scer\UAS} under the control of Scer\GAL4^btl.PS results in the presence of extra terminal cells in the larval tracheal system.

(Jarvis et al., 2006)

Expression of csw::Src64B^{src90.Scer\UAS} under the control of Scer\GAL4^CY2 results in dorsalised eggshells. Expression of csw::Src64B^{src90.Scer\UAS} under the control of Scer\GAL4^55B results in dorsalised embryos. Expression of csw::Src64B^{src90.Scer\UAS} under the control of Scer\GAL4^71B results in ectopic wing veins. Expression of csw::Src64B^{src90.Scer\UAS} under the control of Scer\GAL4^twi.PG results in an increase in the number of eve-positive mesodermal cells in the embryo, most likely due to duplication of the DA1 muscle precursors. Expression of two copies of csw::Src64B^{src90.Scer\UAS} under the control of Scer\GAL4^twi.PG results the formation of eve-positive cells outside of their normal boundary. Embryos expressing csw::Src64B^{src90.Scer\UAS} under the control of Scer\GAL4^twi.PG have the normal number of VA2 muscle precursor cells.

(Johnson Hamlet and Perkins, 2001)

Expression driven by various Scer\GAL4 lines causes expansion of ventral ectodermal cell fates, aberrant tracheal cell migration, ectopic tracheal tissue, and increased numbers of select muscle and heart precursor cells.

(Johnson and Perkins, 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^twi.PG, csw^{UAS.Tag:Myr(Src64B)} has mesoderm phenotype, suppressible by msk[+]/msk⁵

(Johnson Hamlet and Perkins, 2001)

NOT Enhancer of

Statement

Reference

Scer\GAL4^twi.PG/csw^{UAS.Tag:Myr(Src64B)} is a non-enhancer of muscle cell of ventral acute muscle 2 | precursor phenotype of pnt^Δ88

(Johnson Hamlet and Perkins, 2001)

Suppressor of

Statement

Reference

Scer\GAL4^twi.PG/csw^{UAS.Tag:Myr(Src64B)} is a suppressor of muscle cell of ventral acute muscle 2 | precursor phenotype of Raf¹¹

(Johnson Hamlet and Perkins, 2001)

Scer\GAL4^twi.PG/csw^{UAS.Tag:Myr(Src64B)} is a suppressor of muscle cell of ventral acute muscle 2 | precursor phenotype of rho^PΔ38

(Johnson Hamlet and Perkins, 2001)

Scer\GAL4^twi.PG/csw^{UAS.Tag:Myr(Src64B)} is a suppressor of muscle cell of ventral acute muscle 2 | precursor phenotype of spi³

(Johnson Hamlet and Perkins, 2001)

csw^{UAS.Tag:Myr(Src64B)}, Scer\GAL4^twi.PG is a suppressor | partially of muscle cell of ventral acute muscle 2 | precursor phenotype of Egfr^DN.UAS.cBa, Scer\GAL4^twi.PG

(Johnson Hamlet and Perkins, 2001)

Scer\GAL4^twi.PG/csw^{UAS.Tag:Myr(Src64B)} is a suppressor of muscle cell of ventral acute muscle 2 | precursor phenotype of Ras85D^ΔC40B

(Johnson Hamlet and Perkins, 2001)

Scer\GAL4^twi.PG/csw^{UAS.Tag:Myr(Src64B)} is a suppressor of muscle cell of A1-7 dorsal acute muscle 1 phenotype of csw⁵

(Johnson Hamlet and Perkins, 2001)

Scer\GAL4^twi.PG/csw^{UAS.Tag:Myr(Src64B)} is a suppressor of muscle cell of ventral acute muscle 2 | precursor phenotype of csw⁵

(Johnson Hamlet and Perkins, 2001)

Scer\GAL4^twi.PG/csw^{UAS.Tag:Myr(Src64B)} is a suppressor of mesoderm phenotype of csw⁵

(Johnson Hamlet and Perkins, 2001)

Scer\GAL4^twi.PG/csw^{UAS.Tag:Myr(Src64B)} is a suppressor of muscle cell of ventral acute muscle 2 | precursor phenotype of csw^LE120

(Johnson Hamlet and Perkins, 2001)

Scer\GAL4^twi.PG/csw^{UAS.Tag:Myr(Src64B)} is a suppressor of muscle cell of ventral acute muscle 2 | precursor phenotype of dos^R31

(Johnson Hamlet and Perkins, 2001)

Scer\GAL4^twi.PG/csw^{UAS.Tag:Myr(Src64B)} is a suppressor of muscle cell of ventral acute muscle 2 | precursor phenotype of ksr^s628

(Johnson Hamlet and Perkins, 2001)

NOT Suppressor of

Statement

Reference

Scer\GAL4^twi.PG/csw^{UAS.Tag:Myr(Src64B)} is a non-suppressor of muscle cell of ventral acute muscle 2 | precursor phenotype of pnt^Δ88

(Johnson Hamlet and Perkins, 2001)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of csw::Src64B^{src90.Scer\UAS} under the control of Scer\GAL4^twi.PG partially suppresses the loss of VA2 muscle precursor cells seen in csw⁵/Y embryos derived from homozygous female germline clones. The eve-positive muscle progenitors and subsequent pericardial and DA1 precursor cells are recovered in csw⁵/Y embryos that are also expressing csw::Src64B^{src90.Scer\UAS} under the control of Scer\GAL4^twi.PG. Expression of csw::Src64B^{src90.Scer\UAS} under the control of Scer\GAL4^twi.PG nearly completely suppresses the loss of VA2 muscle precursor cells seen in csw^LE120/Y embryos derived from homozygous female germline clones (94% of hemisegments have VA2 cells). Expression of csw::Src64B^{src90.Scer\UAS} under the control of Scer\GAL4^twi.PG significantly suppresses the loss of VA2 muscle precursor cells seen in spi³ embryos (67% of hemisegments have VA2 cells). Expression of csw::Src64B^{src90.Scer\UAS} under the control of Scer\GAL4^twi.PG significantly suppresses the loss of VA2 muscle precursor cells seen in rho^PΔ38 embryos (69% of hemisegments have VA2 cells). Co-expression of csw::Src64B^{src90.Scer\UAS} significantly suppresses the loss of VA2 muscle precursor cells seen in embryos expressing Egfr^{DN.Scer\UAS.cBa} under the control of Scer\GAL4^twi.PG (71% of hemisegments have VA2 cells). Expression of csw::Src64B^{src90.Scer\UAS} under the control of Scer\GAL4^twi.PG significantly suppresses the loss of VA2 muscle precursor cells seen in dos^R31 embryos (41% of hemisegments have VA2 cells). Expression of csw::Src64B^{src90.Scer\UAS} under the control of Scer\GAL4^twi.PG partially suppresses the loss of VA2 muscle precursor cells seen in Ras85D^ΔC40B embryos (21% of hemisegments have VA2 cells). Expression of csw::Src64B^{src90.Scer\UAS} under the control of Scer\GAL4^twi.PG partially suppresses the loss of VA2 muscle precursor cells seen in phl¹¹/Y embryos (10.5% of hemisegments have VA2 cells). Expression of csw::Src64B^{src90.Scer\UAS} under the control of Scer\GAL4^twi.PG partially suppresses the loss of VA2 muscle precursor cells seen in ksr^s628 embryos (14% of hemisegments have VA2 cells). msk⁵ dominantly suppresses the formation of extra eve-positive mesodermal cells seen in embryos expressing csw::Src64B^{src90.Scer\UAS} under the control of Scer\GAL4^twi.PG. Expression of csw::Src64B^{src90.Scer\UAS} under the control of Scer\GAL4^twi.PG does not affect the loss of VA2 muscle precursor cells seen in pnt^Δ88 embryos (82% of hemisegments have VA2 cells).

(Johnson Hamlet and Perkins, 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

23877

y¹ w^*; P{UAS-csw.src90}H2M

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Csw^src90

(Johnson and Perkins, 1998)

csw::Src64B^{src90.Scer\UAS}

csw::Src64B^src90.UAS

csw^{UAS.Tag:Myr(Src64B)}

csw^src90.UAS

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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