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General Information
Symbol
Dmel\csw5
Species
D. melanogaster
Name
FlyBase ID
FBal0001924
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
cswVA199
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Glial engulfment activity is normal in csw5 heterozygous flies.

Mutant embryos show a severe loss of eve-positive mesodermal cells. The DA1 muscle is never seen in these embryos later in development. csw5/Y embryos derived from homozygous female germline clones show loss of VA2 muscle precursor cells (only 29% of hemisegments contain VA2 precursor cells).

csw5 germline clone mosaics cause maternal effect lethality. Embryos derived from mothers with germline clones, that exhibit a corkscrew cuticle phenotype (97%) have slight paternal rescue of the germline clone phenotype, embryos that do not receive a wild type copy from their father (3%), null embryos, die with a 'U'-shaped cuticle phenotype. In paternally rescued and null embryos specific terminal structures are entirely deleted and/or malformed. In mutant embryos ventral cells undergo cell fate changes to that of more lateral epidermal cell fates. Null embryos exhibit a severe CNS phenotype: horizontal commissures are collapsed. Paternally rescued embryos exhibit a less severe commissural phenotype. In both embryos the longitudinal tracts are rudimentary and discontinuous. Migration of tracheal precursor cells is defective, the mature trachea is barely recognisable as tracheal tissue. Pupal lethality can be rescued by heat induced expression of P{hs-SHP-2} during larval and pupal stages but not by expression of P{hs-SHP-1}.

Abnormal development of terminal internal structures. Syncytial and cellular blastoderm embryos show reduced posterior tll domain. hb remains as a posterior cap and the seventh ftz stripe expands posteriorly, both due to lack of hkb repressing activity. In germline clones double mutant for phlPB26 and csw5, tll is entirely missing posteriorly.

Lethality occurs during larval and pupal stages. Phenotype of homozygous germ line clones is maternal effect lethal.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference

csw5 has lethal | pupal stage phenotype, suppressible by Hsap\PTPN11hs.PP

NOT suppressed by
Statement
Reference

csw5 has lethal | pupal stage phenotype, non-suppressible by Hsap\PTPN6hs.PP

NOT Enhancer of
Statement
Reference

csw5 is a non-enhancer of visible phenotype of upd1GMR.PB

NOT Suppressor of
Statement
Reference

csw5 is a non-suppressor of visible phenotype of upd1GMR.PB

Phenotype Manifest In
Suppressed by
NOT Enhancer of
Statement
Reference

csw5 is a non-enhancer of eye phenotype of upd1GMR.PB

NOT Suppressor of
Statement
Reference

csw5 is a non-suppressor of eye phenotype of upd1GMR.PB

Other
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

A csw5 heterozygous mutant background completely suppresses the blocked severed axon debris engulfment seen in flies expressing drprII.Scer\UAS in glial cells under the control of Scer\GAL4repo.

Expression of csw::Src64Bsrc90.Scer\UAS under the control of Scer\GAL4twi.PG partially suppresses the loss of VA2 muscle precursor cells seen in csw5/Y embryos derived from homozygous female germline clones. The eve-positive muscle progenitors and subsequent pericardial and DA1 precursor cells are recovered in csw5/Y embryos that are also expressing csw::Src64Bsrc90.Scer\UAS under the control of Scer\GAL4twi.PG.

The extent to which posterior pattern elements are formed directly reflects the magnitude of tor signal. Thus mutants derived from germ line clones can be ordered with respect to increasing posterior abnormalities thus: phl1 (normal posterior patterning) - csw6 - csw6,phl1 - csw5,phlPB26 (deletion of A8).

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Rescued by
Comments

Mutant phenotype can be rescued by cswhs.PP. Fully zygotically rescued adult males are fertile and when crossed to heterozygous females produce both male and female progeny. These females generate eggs with fused dorsal appendages. Examination of rescued adults reveals consistent defects; absence of one or both aristae, lack of one or more of the distal-most leg segments, incomplete formation of the distal portions of wing vein L5, eyes have reduced numbers of disorganised ommatidia and interommatidial bristles.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer

Lefevre.

Comments
Comments

Phenotypic series of csw mutant embryos derived from homozygous germline clones, classified according to cuticular phenotype: csw5 = cswLE120 > csw1 = csw13-87 > cswe13d.3 > csw7 = csw6.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
References (8)