FlyBase Allele Report: Dmel\Raf[PB26]

General Information

Symbol

Dmel\Raf^PB26

Species

D. melanogaster

Name

FlyBase ID

FBal0024634

Feature type

allele

Associated gene

Dmel\Raf

Associated Insertion(s)

Carried in Construct

Also Known As

D-raf^PB26

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Radke et al., 1997, Melnick et al., 1993, Perkins et al., 1992)

Mutagen

ethyl methanesulfonate

(Melnick et al., 1993)

Progenitor genotype

Cytology

Description

Encodes a truncated protein lacking C-terminal kinase residues.

(Radke et al., 1997)

Amino acid replacement: W634term.

(Melnick et al., 1993)

Mutation is within the CR3 domain.

(Melnick et al., 1993)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:2,342,983..2,342,983 [+]

Nucleotide change:

G2342983A

Amino acid change:

W591term | Raf-PA; W591term | Raf-PE

Reported amino acid change:

W634term

Comment:

G to A nucleotide change at the second or third position of the wild type Trp codon leads to a nonsense mutation (exact site of mutation unspecified). The mutation was annotated at the second base of the codon.

(Melnick et al., 1993)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die before end of pupal stage | recessive

(Melnick et al., 1993)

some die during pupal stage | recessive

(Melnick et al., 1993)

Phenotype Manifest In

embryo | anterior

(Radke et al., 1997)

Detailed Description

Statement

Reference

The extent to which posterior pattern elements are formed directly reflects the magnitude of tor signal. Thus mutants derived from germ line clones can be ordered with respect to increasing posterior abnormalities thus: phl¹ (normal posterior patterning) - csw⁶ - csw⁶,phl¹ - csw⁵,phl^PB26 (deletion of A8).

(Ghiglione et al., 1999)

Homozygous embryos exhibit consistently missing head structures and variable tail defects.

(Radke et al., 1997)

Class 2 allele: Unrescued embryos (lacking maternal and zygotic phl activity) differentiate more cuticular elements than class 1 embryos. Rescued embryos (carrying wild type phl from their father) develop more terminal cuticular elements than class 1 embryos: posterior spiracles are present and the A8 denticle band frequently observed and well differentiated. 35% of class 2 rescued embryos are twisted, like csw embryos. fkh expression indicates that hindgut is variably present, though always malformed, and Malpighian tubules are missing. tll and hb expression at the posterior termini of cellular blastoderm embryos is reduced, indicating blastoderm fate map deleted from 0% EL to 10--15%.

(Melnick et al., 1993)

tll expression severely reduced posteriorly. In germline clones double mutant for phl^PB26 and csw⁵, tll is entirely missing posteriorly.

(Perkins et al., 1992)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

Raf^PB26 is a non-enhancer of visible phenotype of Ret^MEN2B.GMR

(Read et al., 2005)

NOT Suppressor of

Statement

Reference

Raf^PB26 is a non-suppressor of visible phenotype of Ret^MEN2B.GMR

(Read et al., 2005)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

Raf^PB26 is a non-enhancer of eye phenotype of Ret^MEN2B.GMR

(Read et al., 2005)

NOT Suppressor of

Statement

Reference

Raf^PB26 is a non-suppressor of eye phenotype of Ret^MEN2B.GMR

(Read et al., 2005)

Other

Statement

Reference

Raf^PB26, csw⁵ has embryonic epidermis | posterior phenotype

(Ghiglione et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Anterior injection of wild type RNA rescues the head structures.

(Radke et al., 1997)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Allele designation of hypomorph based on observation that phl^PB26 homozygotes die as pupae but phl^PB26 heterozygotes with Df(1)64c18 die at larval-pupal interface. Isolated in a screen described in Perrimon et al., Dev. Biol.110: 480.

(Melnick et al., 1993)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

D-raf^PB26

(Ghiglione et al., 1999, Radke et al., 1997, Lu et al., 1993, Melnick et al., 1993)

Draf^PB26

(Li et al., 2002)

Raf^PB26

phl^PB26

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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