Nowaskie, R.R., Kitch, A., Adams, A., Anandaraj, A., Apawan, E., Bañuelos, L., Betz, C.J., Bogunia, J.M., Buechlein, N., Burns, M.R., Collier, H.A., Collins, Z., Combs, K., Dakarian, V.D., Daniel, A., De Jesus Iii, C.M., Erickson, J.D., Estrada, B., Estrada, K., Fields, S., Gabriel, M., Garcia, R.M., Gitamo, S., Granath, E., Hardin, S.N., Hattling, E., Henriquez, A.V., Hernandez, D., Johnson, L., Kim, A.H., Kolley, L.K., Larue, K.M., Lockwood, E., Longoria, N., Lopez, C., Lopez-Roca Fernandez, R.C., Lozano, S., Manthie, C., May, T., Mehrzad, Z., Mendoza, I., Mohan, S., Mounthachak, C., Muyizere, M., Myers, M.R., Newton, J., Nwawueze, A., Paredes, A.J., Pezdek, M.N., Phat Nguyen, H., Pobuda, N., Sadat, S., Sailor, J.J., Santiago, D., Sbarbaro, M., Schultz Iii, D.E., Senobari, A.N., Shouse, E.M., Snarski, S.M., Solano, E., Solis Campos, N., Stewart, E., Szczepaniak, J., Tejeda, M., Teoli, D.F., Tran, M., Trivedi, N., Uribe Aristizabal, L., Vargas, B.Z., Walker Iii, K.W., Wasiqi, J., Wong, J., Zachrel, A., Shah, H.P., Small, E., Watts, C.T., Croonquist, P., Devergne, O., Jones, A.K., Taylor, E.E., Kagey, J.D., Merkle, J.A. (2023). clifford [B.4.1] , an allele of CG1603 , causes tissue overgrowth in the Drosophila melanogaster eye.  MicroPubl Biol 2023(): .

FBrf0257500

Research paper

Mutant B.4.1 , generated via EMS mutagenesis in Drosophila melanogaster , was studied by undergraduate students participating in the Fly-CURE. After inducing genetically mosaic tissue in the adult eye, B.4.1 mutant tissue displays a robust increase in cell division and a rough appearance. Complementation mapping and sequence analysis identified a nonsense mutation in the gene CG1603 , which we named clifford (cliff) due to observed increases in red-pigmented mutant tissue compared to controls. cliff encodes a zinc finger-containing protein implicated in transcriptional control. RNAi knockdown of cliff similarly results in rough eyes, confirming a role for Cliff in eye development.

PMC10481159 (PMC) (EuropePMC)