FlyBase Allele Report: Dmel\Dark[H16]

General Information

Symbol

Dmel\Dark^H16

Species

D. melanogaster

Name

FlyBase ID

FBal0194626

Feature type

allele

Associated gene

Dmel\Dark

Associated Insertion(s)

Carried in Construct

Also Known As

ark^H16

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Srivastava et al., 2007)

loss of function allele

(Herz et al., 2006)

Mutagen

ethyl methanesulfonate

(Srivastava et al., 2007)

Progenitor genotype

Cytology

Description

Amino acid replacement: K42term.

(Srivastava et al., 2007)

Amino acid replacement: ?42term.

(Herz et al., 2006)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:17,021,524..17,021,524 [+]

Nucleotide change:

A17021524T

Amino acid change:

K42term | Dark-PA; K42term | Dark-PB

Reported amino acid change:

K42term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Srivastava et al., 2007)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates carcinoma

modeled by Vps25^N55

(Woodfield et al., 2013, Herz et al., 2006)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell death | embryonic stage | germline clone

(Srivastava et al., 2007)

lethal - all die before end of pupal stage | recessive

(Srivastava et al., 2007)

some die during pupal stage | recessive

(Srivastava et al., 2007)

Phenotype Manifest In

midline glial cell | increased number

(Srivastava et al., 2007)

Detailed Description

Statement

Reference

In Ark^H16 embryos, acridine orange-positive cell death and caspase-3-like activity are significantly reduced, but not completely blocked.

Stage 17 Ark^H16 embryos contain on average 10.7 midline glia cells per segment, compared to 3 such cells in wild type.

(Srivastava et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Dark^H16, Vps25^N55 has abnormal cell growth | somatic clone | cell non-autonomous phenotype

(Herz et al., 2006)

Dark^H16, Scer\GAL4^Tub.PU, Vps25^N55 has increased occurrence of cell division | somatic clone | larval stage phenotype

(Herz et al., 2006)

Dark^H16, Scer\GAL4^Tub.PU, Vps25^N55 has neoplasia | somatic clone | third instar larval stage phenotype

(Herz et al., 2006)

Phenotype Manifest In

Suppressor of

Statement

Reference

Dark^H16 is a suppressor of eye phenotype of hid^GMR.PG

(Srivastava et al., 2007)

Other

Statement

Reference

Dark^H16, Vps25^N55 has eye phenotype

(Herz et al., 2006)

Dark^H16, Scer\GAL4^Tub.PU, Vps25^N55, puc^UAS.cMa has eye phenotype

(Herz et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

The W^GMR.PG eye ablation phenotype is strongly suppressed when eyes carry homozygous clones for Ark^H16.

(Srivastava et al., 2007)

Vps25^N55/Ark^H16 mosaics exhibit non-autonomous cell proliferation. Vps25^N55/Ark^H16 cells undergo apoptosis. Vps25^N55/Ark^H16 clones cannot be recovered in the adult eye. Vps25^N55/Ark^H16 clones occupy a large fraction of the eye discs, suggesting that these clones have no intrinsic growth disadvantage over wild-type tissue if cell death is blocked. The adult eye of Vps25^N55/Ark^H16 mosaics is severely overgrown and folded.

Vps25^N55 Ark^H16/puc^Scer\UAS.cMa mosaic eye discs are severely overgrown.

(Herz et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Ark^H16

Dark^H16

H16

(Srivastava et al., 2007)

ark^H16

(Woodfield et al., 2013, Srivastava et al., 2007, Herz et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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