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Citation
Colussi, P.A., Quinn, L.M., Huang, D.C.S., Coombe, M., Read, S.H., Richardson, H., Kumar, S. (2000). Debcl, a proapoptotic Bcl-2 homologue, is a component of the Drosophila melanogaster cell death machinery.  J. Cell Biol. 148(4): 703--714.
FlyBase ID
FBrf0125437
Publication Type
Research paper
Abstract

Bcl-2 family of proteins are key regulators of apoptosis. Both proapoptotic and antiapoptotic members of this family are found in mammalian cells, but no such proteins have been described in insects. Here, we report the identification and characterization of Debcl, the first Bcl-2 homologue in Drosophila melanogaster. Structurally, Debcl is similar to Bax-like proapoptotic Bcl-2 family members. Ectopic expression of Debcl in cultured cells and in transgenic flies causes apoptosis, which is inhibited by coexpression of the baculovirus caspase inhibitor P35, indicating that Debcl is a proapoptotic protein that functions in a caspase-dependent manner. debcl expression correlates with developmental cell death in specific Drosophila tissues. We also show that debcl genetically interacts with diap1 and dark, and that debcl-mediated apoptosis is not affected by gene dosage of rpr, hid, and grim. Biochemically, Debcl can interact with several mammalian and viral prosurvival Bcl-2 family members, but not with the proapoptotic members, suggesting that it may regulate apoptosis by antagonizing prosurvival Bcl-2 proteins. RNA interference studies indicate that Debcl is required for developmental apoptosis in Drosophila embryos. These results suggest that the main components of the mammalian apoptosis machinery are conserved in insects.

PubMed ID
PubMed Central ID
PMC2169366 (PMC) (EuropePMC)
Related Publication(s)
Note

Drosophila apoptosis and Bcl-2 genes: Outliers fly in.
Chen and Abrams, 2000, J. Cell Biol. 148(4): 625--628 [FBrf0125406]

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    J. Cell Biol.
    Title
    Journal of Cell Biology
    Publication Year
    1966-
    ISBN/ISSN
    0021-9525
    Data From Reference
    Aberrations (4)
    Alleles (7)
    Genes (8)
    Insertions (3)
    Experimental Tools (2)
    Transgenic Constructs (5)