FlyBase Allele Report: Dmel\Dark[CD8]

General Information

Symbol

Dmel\Dark^CD8

Species

D. melanogaster

Name

FlyBase ID

FBal0101816

Feature type

allele

Associated gene

Dmel\Dark

Associated Insertion(s)

P{lacW}Dark^CD8

Carried in Construct

Key Links

Allele class

hypomorphic allele - genetic evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Quinn et al., 2000, Rodriguez et al., 1999)

Mutagen

P-element activity

(Rodriguez et al., 1999)

Progenitor genotype

P{lacW}Dark^k11502

(Rodriguez et al., 1999)

Associated Insertion(s)

P{lacW}Dark^CD8

Cytology

Description

Insertion of a P{lacW} element in the first intron (the P{lacW}Ark^k11502 insertion upstream of the Ark promoter is retained).

(Rodriguez et al., 1999)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased fecundity | recessive

(Rodriguez et al., 1999)

melanotic mass phenotype | recessive

(Rodriguez et al., 1999)

partially lethal

(Rodriguez et al., 1999)

visible | recessive

(Rodriguez et al., 1999)

Phenotype Manifest In

anterior scutellar bristle

(Rodriguez et al., 1999)

macrochaeta

(Rodriguez et al., 1999)

melanotic mass

(Rodriguez et al., 1999)

posterior scutellar bristle

(Rodriguez et al., 1999)

wing

(Rodriguez et al., 1999)

Detailed Description

Statement

Reference

Most homozygotes survive to adulthood, although at a lower frequency than heterozygous siblings. Many homozygotes derived from heterozygous parents have melanotic tumours and abnormalities affecting the wings and/or bristles. Most affected flies have severe wing defects similar to a 'gnarled' or 'wrinkled' phenotype, while others have wing blisters or burnt 'notched' wings. 8% of flies have melanotic tumours inside the body. Among flies with extra bristles, most have one extra anterior scutellar bristle, while a few have two extra anterior scutellar bristles and/or an extra posterior scutellar bristle. Homozygotes derived from homozygous parents have impaired viability and fecundity and have an increased penetrance and expression of similar defects to those seen in homozygotes derived from heterozygous parents.

(Rodriguez et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Dark^CD8 is a suppressor of visible phenotype of Debcl^UAS.Tag:HA, Scer\GAL4^GMR.PF

(Colussi et al., 2000)

Dark^CD8 is a suppressor of visible phenotype of Dronc^UAS.EGFP, Scer\GAL4^GMR.PF

(Quinn et al., 2000)

Phenotype Manifest In

Suppressor of

Statement

Reference

Dark^CD4/Dark^CD8 is a suppressor | partially of border follicle cell phenotype of Rac1^N17.UAS, Scer\GAL4^slbo.2.6

(Geisbrecht and Montell, 2004)

Ark[+]/Dark^CD8 is a suppressor | partially of border follicle cell phenotype of Rac1^N17.UAS, Scer\GAL4^slbo.2.6

(Geisbrecht and Montell, 2004)

Dark^CD8/Dark^CD8 is a suppressor | partially of border follicle cell phenotype of Rac1^N17.UAS, Scer\GAL4^slbo.2.6

(Geisbrecht and Montell, 2004)

Dark^CD8 is a suppressor of eye phenotype of Debcl^UAS.Tag:HA, Scer\GAL4^GMR.PF

(Colussi et al., 2000)

Dark^CD8 is a suppressor of eye phenotype of Dronc^UAS.EGFP, Scer\GAL4^GMR.PF

(Quinn et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

The border follicle cell migration defect seen in egg chambers expressing Rac1^N17.Scer\UAS under the control of Scer\GAL4^slbo.2.6 is partially suppressed by Ark^CD8/+, Ark^CD8/Ark^CD8 or Ark^CD4/Ark^CD8.

(Geisbrecht and Montell, 2004)

Suppresses the eye phenotype of flies expressing debcl^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^GMR.PF.

(Colussi et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Dark^CD4

(Rodriguez et al., 1999)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

23287

y¹ w^*; P{lacW}Dark^CD8/CyO, y⁺

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Ark^CD8

Dark^CD8

dark^CD8

(Wang and Baker, 2015, Cullen and McCall, 2004, Geisbrecht and Montell, 2004, Colussi et al., 2000, Quinn et al., 2000)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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