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General Information
Symbol
Dmel\DroncUAS.EGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0122046
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-dronc, UAS-dronc-GFP
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of wild-type Dronc tagged with EGFP.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of NcScer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4Crz.504 results in apoptosis in all vCrz neurons, but not in proto-cerebral corazonergic neurons.

Approximately 95% of larvae expressing Scer\GAL4Adh.PF-driven NcScer\UAS.T:Avic\GFP-EGFP in the fat body die during different larval stages. The remaining animals die at the pupal stage. A significant portion of fat body cells in these mutants display apoptosis at the early wandering third instar larval stage.

Expression of NcScer\UAS.T:Avic\GFP-EGFP, under the control of Scer\GAL4elav-C155, shortens lifespan in flies.

NcScer\UAS.T:Avic\GFP-EGFP; Scer\GAL4hs.PB rescues pupariation of most Nc30, Nc11 or Nc51 homozygous larvae. It also rescues cell death in the larval midgut in response to feeding mid-third instar larvae with ecdysone and the normal pattern of cell death of abdominal neuroblasts in the late third instar.

When NcScer\UAS.T:Avic\GFP-EGFP is driven by Scer\GAL4GMR.PF, animals have small mottled eyes.

Expression of NcScer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4GMR.PF results in massive induction of cell death in the posterior part of the eye imaginal disc. Expression of NcScer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4hs.PB during embryogenesis or larval development using heat shock results in ectopic cell death. Many animals expressing NcScer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4GMR.PF die as pupae. The few adults that survive have severely ablated eyes. Insertions of P{UAS-Nc.EGFP} that result in less lethality when expressed under the control of Scer\GAL4GMR.PF give rise to adult flies with slightly rough and mottled eyes at 25oC. The phenotype is more severe at 29oC.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
NOT Enhanced by
Statement
Reference
Suppressed by
NOT suppressed by
Other
Phenotype Manifest In
Enhanced by
NOT Enhanced by
Suppressed by
NOT suppressed by
Other
Additional Comments
Genetic Interactions
Statement
Reference

Wing discs coexpressing BacA\p35Scer\UAS.cHa and NcScer\UAS.T:Avic\GFP-EGFP under the control of either Scer\GAL4en-e16E or Scer\GAL4ptc-559.1 show increased cell proliferation in the domain of Scer\GAL4 expression.

Xenogenetic Interactions
Statement
Reference

Co-expression of BacA\p35Scer\UAS.cHa with NcScer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4Crz.504 does not prevent the Nc-induced apoptosis in vCrz neurons.

Expression of NcScer\UAS.T:Avic\GFP-EGFP and BacA\p35Scer\UAS.cHa in the wing disc, driven by Scer\GAL4en-e16E, induces a strong overproliferation phenotype.

Complementation and Rescue Data
Comments

NcScer\UAS.T:Avic\GFP-EGFP; Scer\GAL4hs.PB rescues pupariation of most Nc30, Nc11 or Nc51 homozygous larvae. It also rescues cell death in the larval midgut in response to feeding mid-thrd instar larvae with ecdysone and the normal pattern of cell death of abdominal neuroblasts in the late third instar.

Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
DroncScer\UAS.T:Avic\GFP-EGFP
DroncUAS.EGFP
NcScer\UAS.T:Avic\GFP-EGFP
NcScer\UAS.T:Avic\GFP-F64L.S65T
Name Synonyms
Secondary FlyBase IDs
    References (11)