FlyBase Allele Report: Hsap\MAPT[UAS.2N4R.cSa]

General Information

Symbol

Hsap\MAPT^UAS.2N4R.cSa

Species

H. sapiens

Name

Saccharomyces cerevisiae UAS construct a of Santa-Maria

FlyBase ID

FBal0302694

Feature type

allele

Associated gene

Hsap\MAPT

Associated Insertion(s)

Carried in Construct

P{UAS-MAPT.2N4R.S}

Also Known As

UAS-hTau

Key Links

Transgenic product class

wild_type

(Santa-Maria et al., 2015)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Santa-Maria et al., 2015)

Mutagen

in vitro construct

(Santa-Maria et al., 2015)

Progenitor genotype

Carried in construct

P{UAS-MAPT.2N4R.S}

Cytology

Description

UAS regulatory sequences drive expression of the 2N4R isoform of Hsap\MAPT.

(Santa-Maria et al., 2015)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Hsap\MAPT

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of tauopathy

CEA

(Kim et al., 2023, Asadzadeh et al., 2022, Santa-Maria et al., 2015)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of tauopathy

is exacerbated by Nsun2^HMJ24019

(Kim et al., 2023)

is ameliorated by Nsun2^UAS.cAa

(Kim et al., 2023)

is ameliorated by mir-219^{UAS.DsRed(Unk)}

(Santa-Maria et al., 2015)

is exacerbated by mir-219^{sponge.UAS.EGFP}

(Santa-Maria et al., 2015)

is ameliorated by Dronc^HMS00758

(Asadzadeh et al., 2022)

is exacerbated by Dronc^UAS.EGFP

(Asadzadeh et al., 2022)

is exacerbated by Hsap\VPS26A^UAS.Tag:HA

(Asadzadeh et al., 2022)

is exacerbated by Hsap\VPS29^UAS.cAa

(Asadzadeh et al., 2022)

is exacerbated by Hsap\VPS35^UAS.Tag:HA

(Asadzadeh et al., 2022)

is exacerbated by Rab7^{T22N.UASp.YFP}

(Asadzadeh et al., 2022)

is exacerbated by Vps26^HMS01769

(Asadzadeh et al., 2022)

is exacerbated by Vps29^HMS01877

(Asadzadeh et al., 2022)

is exacerbated by Vps35^HMS01858

(Asadzadeh et al., 2022)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^GMR.PU

(Santa-Maria et al., 2015)

visible | adult stage, with Scer\GAL4^GMR.PU

(Kim et al., 2023)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PU

(Kim et al., 2023, Santa-Maria et al., 2015)

Detailed Description

Statement

Reference

Expression of Hsap\MAPT^{Scer\UAS.2N4R.cSa} under the control of Scer\GAL4^GMR.PU results in a severe rough eye phenotype.

(Santa-Maria et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Hsap\MAPT^UAS.2N4R.cSa, Scer\GAL4^GMR.PU has visible | adult stage phenotype, enhanceable by Nsun2^HMJ24019, Scer\GAL4^GMR.PU

(Kim et al., 2023)

Hsap\MAPT^UAS.2N4R.cSa, Scer\GAL4^GMR.PU has visible phenotype, enhanceable by mir-219^{sponge.UAS.EGFP}, Scer\GAL4^GMR.PU

(Santa-Maria et al., 2015)

NOT Enhanced by

Statement

Reference

Hsap\MAPT^UAS.2N4R.cSa, Scer\GAL4^GMR.PU has visible phenotype, non-enhanceable by Disc\RFP^{mCh.UAS.SCRAMBLE.sponge}, Scer\GAL4^GMR.PU

(Santa-Maria et al., 2015)

Suppressed by

Statement

Reference

Hsap\MAPT^UAS.2N4R.cSa, Scer\GAL4^GMR.PU has visible | adult stage phenotype, suppressible | partially by Nsun2^UAS.cAa, Scer\GAL4^GMR.PU

(Kim et al., 2023)

Hsap\MAPT^UAS.2N4R.cSa, Scer\GAL4^GMR.PU has visible phenotype, suppressible | partially by mir-219^{UAS.DsRed(Unk)}, Scer\GAL4^GMR.PU

(Santa-Maria et al., 2015)

NOT suppressed by

Statement

Reference

Hsap\MAPT^UAS.2N4R.cSa, Scer\GAL4^GMR.PU has visible phenotype, non-suppressible by mir-219^{scrambled.UAS.DsRed(Unk)}, Scer\GAL4^GMR.PU

(Santa-Maria et al., 2015)

Phenotype Manifest In

Enhanced by

Statement

Reference

Hsap\MAPT^UAS.2N4R.cSa, Scer\GAL4^GMR.PU has eye phenotype, enhanceable by Nsun2^HMJ24019, Scer\GAL4^GMR.PU

(Kim et al., 2023)

Hsap\MAPT^UAS.2N4R.cSa, Scer\GAL4^GMR.PU has eye phenotype, enhanceable by mir-219^{sponge.UAS.EGFP}, Scer\GAL4^GMR.PU

(Santa-Maria et al., 2015)

NOT Enhanced by

Statement

Reference

Hsap\MAPT^UAS.2N4R.cSa, Scer\GAL4^GMR.PU has eye phenotype, non-enhanceable by Disc\RFP^{mCh.UAS.SCRAMBLE.sponge}, Scer\GAL4^GMR.PU

(Santa-Maria et al., 2015)

Suppressed by

Statement

Reference

Hsap\MAPT^UAS.2N4R.cSa, Scer\GAL4^GMR.PU has eye phenotype, suppressible | partially by Nsun2^UAS.cAa, Scer\GAL4^GMR.PU

(Kim et al., 2023)

Hsap\MAPT^UAS.2N4R.cSa, Scer\GAL4^GMR.PU has eye phenotype, suppressible | partially by mir-219^{UAS.DsRed(Unk)}, Scer\GAL4^GMR.PU

(Santa-Maria et al., 2015)

NOT suppressed by

Statement

Reference

Hsap\MAPT^UAS.2N4R.cSa, Scer\GAL4^GMR.PU has eye phenotype, non-suppressible by mir-219^{scrambled.UAS.DsRed(Unk)}, Scer\GAL4^GMR.PU

(Santa-Maria et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Expression of mir-219^{Scer\UAS.T:Disc\RFP} partially suppresses the severe rough eye phenotype seen when Hsap\MAPT^{Scer\UAS.2N4R.cSa} is expressed under the control of Scer\GAL4^GMR.PU.

Expression of mir-219^{scrambled.Scer\UAS.T:Disc\RFP} does not suppress the severe rough eye phenotype seen when Hsap\MAPT^{Scer\UAS.2N4R.cSa} is expressed under the control of Scer\GAL4^GMR.PU.

Expression of mir-219^{sponge.Scer\UAS.T:Avic\GFP-EGFP} enhances the severe rough eye phenotype seen when Hsap\MAPT^{Scer\UAS.2N4R.cSa} is expressed under the control of Scer\GAL4^GMR.PU.

Expression of Disc\RFP^{mCh.Scer\UAS.SCRAMBLE.sponge} does not enhance the severe rough eye phenotype seen when Hsap\MAPT^{Scer\UAS.2N4R.cSa} is expressed under the control of Scer\GAL4^GMR.PU.

(Santa-Maria et al., 2015)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Hsap\MAPT^{Scer\UAS.2N4R.cSa}

Hsap\MAPT^UAS.2N4R.cSa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Santa-Maria

Secondary FlyBase IDs

References (3)

Report Sections

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