FlyBase Insertion Report: Dmel\P{lacW}Dark[k11502]

General Information

Symbol

Dmel\P{lacW}Dark^k11502

Species

D. melanogaster

Name

FlyBase ID

FBti0006473

Feature type

transposable_element_insertion_site

Description

Inserted Element

P{lacW}

LINE ID

l(2)k11502

Affected gene(s)

Dark, Ecol\lacZ

Viability / fertility

viable

Associated allele(s)

Dark^k11502, Ecol\lacZ^Dark-k11502

Stock availability

3 publicly available

Genomic Location

Chromosomal Location

2R ( 53E4 )

Sequence Location

2R:17,020,073..17,020,073 [+]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{lacW}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{lacW}; most lines have a lethal or sterile phenotype. The P{lacW} construct carries a w^+mC mini-white visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue (FBrf0049800).

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

2R:17,020,073..17,020,073 [+]

(BDGP Project Members, 1994-1999)

Orientation

Cytological location (computed by FlyBase)

53E4 (inferred by FlyBase from sequence location)

Cytological location (reported)

53F1-53F2 (in situ hybridization reported)

(BDGP Project Members, 1994-1999)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

AQ025966

(FlyBase, 1992-)

Inserted Element

Construct

P{lacW}

Size (Kb)

10.691

Component Allele(s)

w^+mC

Molecular map

Affected Gene(s)

Insertion may affect gene

Dark

(Kanuka et al., 1999, BDGP Project Members, 1994-1999)

Ecol\lacZ

(Arama et al., 2003, Zhou et al., 1999, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Dark^k11502

Phenotypic Class Phenotype Manifest in Detailed Description

Ecol\lacZ^Dark-k11502

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

organism

(Zhou et al., 1999)

embryonic stage 10 -- 11

procephalic neurogenic region

(Zhou et al., 1999)

head mesoderm

(Zhou et al., 1999)

embryonic stage 11

organism | segmentally repeated

(Zhou et al., 1999)

embryonic stage 11 -- 17

embryonic/larval plasmatocyte

(Zhou et al., 1999)

Additional Information

Statement

Reference

A basal level of expression is observed all over the embryo. Elevated levels are observed in the procephalic region. Later expression is observed in a segmentally repeated pattern and in macrophages.

(Zhou et al., 1999)

Marker for

Reflects expression of

Reporter construct used in assay

Data on Genetic Line

Line ID

l(2)k11502

(Gene Disruption Project members, 2001-)

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (3)

Bloomington

11041

y¹ w^67c23; P{lacW}Dark^k11502/CyO

23285

y¹ w^*; P{lacW}Dark^k11502 Dark⁸²/CyO, y⁺

Kyoto

111308

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{neoFRT}42D P{lacW}Dark^k11502 /CyO y⁺

External Crossreferences and Linkouts ( 0 )

Comments

Location 2R:12534936-12534937 confirmed by FlyBase alignment of dbGSS accession AQ025966 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.

(FlyBase, 2005)

Insertion separable from lethal phenotype induced on same chromosome.

(Rodriguez et al., 1999)

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

P{lacW}Ark^k11502

(FlyBase, 2005, FlyBase, 1992-)

P{lacW}Dark^k11502

P{lacW}k11502

(FlyBase, 1992-)

P{lacW}l(2)k11502^k11502

(BDGP Project Members, 1994-1999, FlyBase, 1992-)

Name Synonyms

Secondary FlyBase IDs

References (17)

Report Sections

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