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General Information
Symbol
Dmel\Uba1B2
Species
D. melanogaster
Name
FlyBase ID
FBal0197418
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Nucleotide change:

C9689750T

Amino acid change:

P884L | Uba1-PA; P701L | Uba1-PC

Reported amino acid change:

P884L

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: P884L.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Uba1B2/+ mutant flies display a significant decrease in lifespan as compared to wild type.

Most Uba1B2/Uba1B2 mutants die during late larval or pupal stages. The few escapers are infertile and display , climbing defects and visible abnormalities including unusually thick wing veins.

Uba1B2 homozygous and Uba1B1/Uba1B2 trans-heterozygous eye discs exhibit a significant increase in proliferation (as observed through phosphorylated His3 signal). Ectopic proliferation posterior to the morphogenetic furrow is significant enough that the region of frequent anti-pHis3 staining extends two to three times as far into the region posterior to the morphogenetic furrow.

Homozygous clones in the eye have a growth advantage compared to surrounding wild-type tissue at 18oC. At 30oC, homozygous clones in the eye result in overgrowth of surrounding wild-type tissue.

Homozygous clones in the pupal retina 46 hours after puparium formation contain additional interommatidial cells compared to control retinas (where these extra cells are eliminated by apoptosis during the mid-pupal phase).

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference

Uba1B1/Uba1B2 has lethal phenotype, suppressible by Ras85De1B/Ras85D[+]

Ras85De1B/Ras85D[+], Uba1B1/Uba1B2 has viable phenotype, suppressible by egrUAS.cMa/Scer\GAL4GMR.PF

NOT suppressed by
Statement
Reference

Uba1B1/Uba1B2 has lethal phenotype, non-suppressible by Egfr[+]/Egfrk05115

Uba1B1/Uba1B2 has lethal phenotype, non-suppressible by drkk02401/drk[+]

Uba1B1/Uba1B2 has lethal phenotype, non-suppressible by Sose4G/Sos[+]

Suppressor of
Phenotype Manifest In
Suppressed by
Statement
Reference
Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference

The cell-autonomous overgrowth phenotype found in Uba1B2 homozygous and Uba1B1/Uba1B2 trans-heterozygous eye discs is suppressed in a Ras85De1B heterozygous background.

A Ras85De1B/+ background dramatically suppresses the lethality found in Uba1B1/Uba1B2 homozygotes.

Uba1B1/Uba1B2 eyes in a Ras85De1B heterozygous background show decreased suppression of egrScer\UAS.cMa (under the control of Scer\GAL4GMR.PF) induced cell death.

Overall cell proliferation in Uba1B1/Uba1B2 eye discs is reduced in a Ras85De1B/+ background. Ectopic proliferation posterior to the morphogenetic furrow decreases and is restricted to a thin stripe.

A Egfrk05115/+, drkk02401/+, or Sose4G/+ background does not suppress the ectopic cell divisions found in Uba1B1/Uba1B2 trans-heterozygous eye discs.

The loss of eye tissue that is caused by expression of egrScer\UAS.cMa under the control of Scer\GAL4GMR.PF is partially suppressed if the eye is also homozygous for Uba1B2.

The loss of eye tissue that is caused by WGMR.PG is partially suppressed if the eye is also homozygous for Uba1B2.

The loss of eye tissue that is caused by grimGMR.PC is partially suppressed if the eye is also homozygous for Uba1B2.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (3)