FB2026_02 , released June 18, 2026
FB2026_02 , released June 18, 2026
Gene: Dmel\Uba1
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General Information
Symbol
Dmel\Uba1
Species
D. melanogaster
Name
Ubiquitin activating enzyme 1
Annotation Symbol
CG1782
Feature Type
protein_coding_gene
FlyBase ID
FBgn0023143
Gene Model Status
Current
Stock Availability
25 publicly available
Enzyme Name (EC)
E1 ubiquitin-activating enzyme (6.2.1.45)
Gene Summary
Ubiquitin activating enzyme 1 (Uba1) encodes an E1 enzyme involved in protein ubiquitylation. It contributes to multiple processes including regulation of Ras-ERK signaling, autophagy, apoptosis and tissue growth. [Date last reviewed: 2019-07-11] (FlyBase Gene Snapshot)
All Summaries
Also Known As

E1, UBA-1, l(2)03405, Uba 1, l(2)05642

Key Links
Genomic Location
Cytogenetic map
46A1-46A1
Sequence location
Recombination map
2-61
RefSeq locus
NT_033778 REGION:9688289..9693328
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (20 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
enables ubiquitin activating enzyme activity
inferred from direct assay
(Chang et al., 2013)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
enables ubiquitin-like modifier activating enzyme activity
inferred from electronic annotation with InterPro:IPR000011, InterPro:IPR035985
(InterPro Project Members, 2004-)
Biological Process (15 terms)
Terms Based on Experimental Evidence (13 terms)
CV Term
Evidence
References
involved_in follicle cell of egg chamber development
inferred from mutant phenotype
(Jia et al., 2015)
involved_in glucose homeostasis
inferred from mutant phenotype
(Ugrankar et al., 2015)
involved_in larval midgut cell programmed cell death
inferred from mutant phenotype
(Chang et al., 2013)
involved_in lipid storage
inferred from direct assay
(Beller et al., 2008)
involved_in macroautophagy
inferred from mutant phenotype
(Chang et al., 2013)
involved_in mitophagy
inferred from mutant phenotype
(Chang et al., 2013)
involved_in mushroom body development
inferred from mutant phenotype
(Schuldiner et al., 2008)
involved_in neuron remodeling
inferred from mutant phenotype
(Watts et al., 2003)
involved_in protein polyubiquitination
inferred from mutant phenotype
(Lee et al., 2014)
involved_in protein ubiquitination
inferred from direct assay
(Chang et al., 2013)
inferred from mutant phenotype
(Yan et al., 2009, Lee et al., 2008)
involved_in regulation of growth
inferred from mutant phenotype
(Lee et al., 2008)
involved_in regulation of programmed cell death
inferred from mutant phenotype
(Lee et al., 2008)
inferred from genetic interaction with FLYBASE:hid; FB:FBgn0003997
(Lee et al., 2008)
involved_in regulation of Ras protein signal transduction
inferred from mutant phenotype
(Yan et al., 2009)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
involved_in DNA damage response
inferred from biological aspect of ancestor with PANTHER:PTN000102404
(GO Reference Genome Project, 2011-)
involved_in ubiquitin-dependent protein catabolic process
inferred from biological aspect of ancestor with PANTHER:PTN000102404
(GO Reference Genome Project, 2011-)
Cellular Component (3 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
located_in dendrite
inferred from mutant phenotype
(Kuo et al., 2005)
located_in plasma membrane
inferred from high throughput direct assay
(Tan et al., 2009)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
is_active_in nucleus
inferred from biological aspect of ancestor with PANTHER:PTN000102404
(GO Reference Genome Project, 2011-)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Catalytic Activity (EC/Rhea)
ubiquitin activating enzyme activity
ATP + ubiquitin + [E1 ubiquitin-activating enzyme]-L-cysteine = AMP + diphosphate + S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine (6.2.1.45)
Summaries
Gene Snapshot
Ubiquitin activating enzyme 1 (Uba1) encodes an E1 enzyme involved in protein ubiquitylation. It contributes to multiple processes including regulation of Ras-ERK signaling, autophagy, apoptosis and tissue growth. [Date last reviewed: 2019-07-11]
Gene Group (FlyBase)
UBIQUITIN ACTIVATING ENZYMES -
Ubiquitin activating enzymes are 'canonical' E1 enzymes that activate ubiquitin for subsequent steps along the ubiquitin conjugation cascade. They perform three distinct chemical reactions: first, they bind ATP, magnesium and ubiquitin to catalyze formation of a high-energy acyl adenylate intermediate with subsequent release of pyrophosphate; second, an E1 catalytic cysteine attacks the ubiquitin-adenylate to catalyze formation of a high-energy thioester bond between the E1 and ubiquitin and release of the AMP product; finally, the E1-ubiquitin catalyzes transthioesterification to an E2 to form a high energy thioester-linked E2-ubiquitin product. (Adapted from PMID:28234446.)
Summary (Interactive Fly)

Ubiquitin-activating enzyme, E1, activates and transfers ubiquitin to ubiquitin-conjugating enzymes, tumor suppressor

Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
2

Please see the JBrowse view of Dmel\Uba1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q8T0L3)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Gene model reviewed during 5.42

Gene model reviewed during 5.49

Low-frequency RNA-Seq exon junction(s) not annotated.

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Uba1-RA
FBtr0088499
NM_057962
4343
1191
Uba1-RC
FBtr0339517
NM_001273902
4343
1008
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
UniProt
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Uba1 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

1.19

Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 3
organism | ubiquitous

Comment: maternally deposited

(Fisher et al., 2012)
embryonic stage 4 -- 6
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 7 -- 8
dorsal ectoderm
(Fisher et al., 2012)
trunk mesoderm
(Fisher et al., 2012)
ventral ectoderm
(Fisher et al., 2012)
embryonic stage 9 -- 10
antennal primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
central brain primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
visual primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
dorsal head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
lateral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
ventral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
head mesoderm
(Fisher et al., 2012)
organism | ubiquitous
(Fisher et al., 2012)
trunk mesoderm
(Fisher et al., 2012)
ventral ectoderm
(Fisher et al., 2012)
ventral nerve cord primordium
(Fisher et al., 2012)
embryonic stage 11 -- 12
central brain primordium
(Fisher et al., 2012)
embryonic central brain glial cell
(Fisher et al., 2012)
embryonic central brain neuron
(Fisher et al., 2012)
hindgut proper primordium
(Fisher et al., 2012)
larval lateral VNC glial cell
(Fisher et al., 2012)
lateral cord neuron
(Fisher et al., 2012)
Malpighian tubule primordium
(Fisher et al., 2012)
organism | ubiquitous
(Fisher et al., 2012)
ventral nerve cord primordium
(Fisher et al., 2012)
embryonic stage 13 -- 16
embryonic brain
(Fisher et al., 2012)
embryonic hindgut
(Fisher et al., 2012)
embryonic/larval midgut
(Fisher et al., 2012)
larval nervous system
(Fisher et al., 2012)
gonad
(Fisher et al., 2012)
larval ventral nerve cord
(Fisher et al., 2012)
late third instar larval stage
gamma Kenyon cell | faint
(Hoopfer et al., 2008)
prepupal stage P1
gamma Kenyon cell
(Hoopfer et al., 2008)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
day 5 of adulthood | male -- day 7 of adulthood | male
spermatozoon
(Wasbrough et al., 2010)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in dendrite
inferred from mutant phenotype
(Kuo et al., 2005)
located_in plasma membrane
inferred from high throughput direct assay
(Tan et al., 2009)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Uba1 in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 4-6
  • Stages(s) 7-8
  • Stages(s) 13-16
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 43 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 13 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Uba1
Transgenic constructs containing regulatory region of Uba1
Aberrations (Deficiencies and Duplications) ( 2 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
cell lethal | somatic clone | heat sensitive
lethal
lethal (with Uba103405)
lethal (with Uba1D6)
lethal (with Uba1H33)
lethal (with Uba1H42)
lethal (with Uba1s3484)
lethal (with Uba1UY3010)
lethal | recessive
lethal - all die before end of P-stage | recessive
lethal - all die before end of pupal stage
partially lethal - majority die
viable, with Scer\GAL4da.G32
viable, with Scer\GAL4ptc.PU
viable, with Scer\GAL4vg.PM
viable (with Uba1s3484), with Scer\GAL4da.G32
viable (with Uba1UY3010), with Scer\GAL4da.G32
Sterility
Allele
sterile
sterile (with Uba1B1)
sterile (with Uba1B2)
Other Phenotypes
Allele
abnormal locomotor behavior
abnormal locomotor behavior (with Uba1B1)
abnormal locomotor behavior (with Uba1B2)
abnormal neuroanatomy | adult stage, with Scer\GAL4GMR71G10, Spyo\Cas9UAS.Tag:NLS(Unk)
abnormal neuroanatomy | larval stage, with Scer\GAL4elav-C155
abnormal neuroanatomy | pupal stage
abnormal neuroanatomy | somatic clone
abnormal size | adult stage
chemical sensitive, with Scer\GAL4elav.PU
decreased cell death | pupal stage | somatic clone
decreased occurrence of cell division | larval stage | somatic clone
increased cell death | pupal stage | somatic clone | heat sensitive
increased cell number | cell non-autonomous | somatic clone
increased cell size | larval stage | somatic clone, with Scer\GAL4Act.PU
increased occurrence of cell division | cell non-autonomous | larval stage | somatic clone
increased occurrence of cell division | larval stage (with Uba1B1)
increased occurrence of cell division | larval stage (with Uba1B2)
increased size | adult stage, with Scer\GAL4GMR.PF
short lived, with Scer\GAL4elav.PU
short lived | dominant
some die during pupal stage
visible (with Uba1B1)
visible (with Uba1B2)
visible | recessive
visible | somatic clone
visible | somatic clone | heat sensitive
Phenotype manifest in
Allele
adult mushroom body | somatic clone
anterior fascicle & synapse, with Scer\GAL4elav-C155
anterior fascicle & synapse | supernumerary, with Scer\GAL4elav-C155
axon | adult stage, with Scer\GAL4GMR71G10, Spyo\Cas9UAS.Tag:NLS(Unk)
axon | adult stage | somatic clone
dendrite | somatic clone
dendritic arborizing neuron | P-stage
dopaminergic neuron | chemical conditional, with Scer\GAL4elav.PU
dorsocentral bristle
embryonic/larval fat body | third instar larval stage, with Scer\GAL4SPARC-MI00329-GAL4
embryonic/larval midgut cell | larval stage | somatic clone, with Scer\GAL4Act.PU
extracellular matrix | third instar larval stage, with Scer\GAL4SPARC-MI00329-GAL4
eye, with Scer\GAL4GMR.PF
eye | cell non-autonomous | somatic clone
eye | somatic clone
eye | somatic clone | heat sensitive
eye-antennal disc | somatic clone | third instar larval stage | heat sensitive
eye disc | cell non-autonomous | somatic clone
eye disc | pupal stage | somatic clone
eye disc | somatic clone
eye disc posterior to the morphogenetic furrow
eye disc posterior to the morphogenetic furrow (with Uba1B1)
eye disc posterior to the morphogenetic furrow (with Uba1B2)
gamma Kenyon cell | adult stage, with Scer\GAL4GMR71G10, Spyo\Cas9UAS.Tag:NLS(Unk)
humeral disc
interommatidial cell | pupal stage | somatic clone
larval intersegmental nerve, with Scer\GAL4elav-C155
leg
macrochaeta
mushroom body & axon | somatic clone
neuroblast | somatic clone
proboscis
retina | P-stage | somatic clone
sternopleural bristle | increased number
wing
wing vein
wing vein (with Uba1B1)
wing vein (with Uba1B2)
wing vein | ectopic
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (11)
Hsap\UBA1
13 of 14
Yes
Yes
Hsap\UBA7
7 of 14
No
Yes
1  
Hsap\UBA6
6 of 14
No
Yes
Hsap\MOCS3
2 of 14
No
No
Hsap\NAE1
2 of 14
No
No
Hsap\SAE1
2 of 14
No
No
Hsap\UBA2
2 of 14
No
No
Hsap\UBA3
2 of 14
No
No
Hsap\UBA5
2 of 14
No
No
18  
Hsap\ATG7
1 of 14
No
No
Hsap\CENPC
1 of 14
No
Yes
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (13)
Rnor\Uba1
13 of 14
Yes
Yes
Rnor\Uba1y
11 of 14
No
Yes
Rnor\Uba6
6 of 14
No
Yes
Rnor\Uba7
6 of 14
No
Yes
Rnor\Mocs3
2 of 14
No
No
Rnor\Sae1
2 of 14
No
No
Rnor\Uba2
2 of 14
No
No
Rnor\Uba3
2 of 14
No
No
Rnor\Uba5
2 of 14
No
No
Rnor\Atg7
1 of 14
No
No
Rnor\Cenpc
1 of 14
No
Yes
Rnor\LOC103694557
1 of 14
No
Yes
Rnor\Nae1
1 of 14
No
No
Mus musculus (laboratory mouse) (12)
Mmus\Uba1
13 of 14
Yes
Yes
Mmus\Uba1y
11 of 14
No
Yes
Mmus\Uba7
7 of 14
No
Yes
Mmus\Uba6
5 of 14
No
Yes
Mmus\Mocs3
2 of 14
No
No
Mmus\Nae1
2 of 14
No
No
Mmus\Sae1
2 of 14
No
No
Mmus\Uba2
2 of 14
No
No
Mmus\Uba3
2 of 14
No
No
Mmus\Uba5
2 of 14
No
No
Mmus\Atg7
1 of 14
No
No
Mmus\Cenpc1
1 of 14
No
Yes
Xenopus tropicalis (Western clawed frog) (8)
Xtro\uba1
6 of 13
Yes
Yes
Xtro\uba6
2 of 13
No
Yes
Xtro\mocs3
1 of 13
No
No
Xtro\nae1
1 of 13
No
No
Xtro\qtrt1
1 of 13
No
No
Xtro\sae1
1 of 13
No
No
Xtro\uba2
1 of 13
No
No
Xtro\uba5
1 of 13
No
No
Danio rerio (Zebrafish) (9)
Drer\uba1
13 of 14
Yes
Yes
Drer\uba7
8 of 14
No
Yes
Drer\uba6
5 of 14
No
Yes
Drer\mocs3
2 of 14
No
No
Drer\nae1
2 of 14
No
No
Drer\sae1
2 of 14
No
No
Drer\uba3
2 of 14
No
No
Drer\uba2
1 of 14
No
No
Drer\uba5
1 of 14
No
No
Caenorhabditis elegans (Nematode, roundworm) (8)
Cele\uba-1
13 of 14
Yes
Yes
Cele\aos-1
2 of 14
No
No
Cele\moc-3
2 of 14
No
No
Cele\rfl-1
2 of 14
No
No
Cele\uba-2
2 of 14
No
No
Cele\uba-5
2 of 14
No
No
Cele\ula-1
2 of 14
No
No
Cele\atg-7
1 of 14
No
No
Anopheles gambiae (African malaria mosquito) (8)
Agam\AgaP_AGAP011872
8 of 12
Yes
Yes
Agam\AgaP_AGAP005102
2 of 12
No
No
Agam\AgaP_AGAP005544
2 of 12
No
No
Agam\AgaP_AGAP008637
2 of 12
No
No
Agam\AgaP_AGAP009012
2 of 12
No
No
Agam\MOCS3_ANOGA
2 of 12
No
No
Agam\AgaP_AGAP004459
1 of 12
No
No
Agam\AgaP_AGAP010303
1 of 12
No
No
Arabidopsis thaliana (thale-cress) (15)
Atha\UBA1
12 of 13
Yes
Yes
Atha\UBA 2
12 of 13
Yes
Yes
Atha\APG7
2 of 13
No
No
Atha\AT1G05350
2 of 13
No
No
Atha\AXL
2 of 13
No
No
Atha\AXR1
2 of 13
No
No
Atha\CNX5
2 of 13
No
No
Atha\ECR1
2 of 13
No
No
Atha\SAE1A
2 of 13
No
No
Atha\SAE1B
2 of 13
No
No
Atha\SAE1B
2 of 13
No
No
Atha\SAE2
2 of 13
No
No
Atha\AT3G25880
1 of 13
No
No
Atha\AT5G19370
1 of 13
No
No
Atha\AT5G37530
1 of 13
No
Yes
Saccharomyces cerevisiae (Brewer's yeast) (9)
Scer\UBA1
13 of 13
Yes
Yes
Scer\AOS1
2 of 13
No
No
Scer\ATG7
2 of 13
No
No
Scer\UBA2
2 of 13
No
No
Scer\UBA3
2 of 13
No
No
Scer\UBA4
2 of 13
No
No
Scer\ULA1
2 of 13
No
No
Scer\TCD1
1 of 13
No
Yes
Scer\TCD2
1 of 13
No
No
Schizosaccharomyces pombe (Fission yeast) (8)
Spom\ptr3
12 of 12
Yes
Yes
Spom\fub2
2 of 12
No
No
Spom\rad31
2 of 12
No
No
Spom\SPAC2G11.10c
2 of 12
No
No
Spom\uba3
2 of 12
No
No
Spom\uba5
2 of 12
No
No
Spom\atg7
1 of 12
No
No
Spom\SPAC1A6.10
1 of 12
No
Yes
Escherichia coli (enterobacterium) (3)
Ecol\moeB
3 of 11
Yes
No
Ecol\thiF
3 of 11
Yes
No
Ecol\tcdA
1 of 11
No
Yes
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:Uba1. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (8)
Aos1
4 of 13
APP-BP1
4 of 13
Mocs3
4 of 13
Uba2
4 of 13
Uba3
4 of 13
Uba5
4 of 13
Atg7
3 of 13
mRpL36
1 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 2 )
    Modifiers Based on Experimental Evidence ( 4 )
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    UBA1; ubiquitin like modifier activating enzyme 1
    13 of 14
    314370
    UBA7; ubiquitin like modifier activating enzyme 7
    7 of 14
    191325
         
        UBA6; ubiquitin like modifier activating enzyme 6
        6 of 14
        611361
            Functional Complementation Data
            Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
            Interactions
            Summary of Physical Interactions
            Interaction Browsers
            View in FlyBase Interactions Browser   View in MIST tool (external link)
            Please see the Physical Interaction reports below for full details
            protein-protein
            Physical Interaction
            Assay
            References
            Uba1 - ben
            experimental knowledge based
            (Guruharsha et al., 2011)
            Summary of Genetic Interactions
            Interaction Browsers
            View in FlyBase Interactions Browser   View in MIST tool (external link)
            Please look at the allele data for full details of the genetic interactions
            Starting gene(s)
            Interaction type
            Interacting gene(s)
            Reference
            Uba1
            suppressible
            Diap1
            (Pfleger et al., 2007)
            Uba1
            suppressible
            N
            (Lee et al., 2008)
            Uba1
            suppressible
            Ras85D
            (Liu and Pfleger, 2013, Yan et al., 2009)
            Uba1
            suppressible
            Stat92E
            (Lee et al., 2008)
            Starting gene(s)
            Interaction type
            Interacting gene(s)
            Reference
            Atg1
            enhanceable
            Uba1
            (Umargamwala et al., 2025)
            Atg1
            suppressible
            Uba1
            (Umargamwala et al., 2025)
            bchs
            enhanceable
            Uba1
            (Simonsen et al., 2007)
            Dcp-1
            enhanceable
            Uba1
            (Kim et al., 2010)
            Debcl
            suppressible
            Uba1
            (Colin et al., 2014)
            egr
            suppressible
            Uba1
            (Pfleger et al., 2007)
            grim::rpr
            enhanceable
            Uba1
            (Wing et al., 2002)
            grim
            suppressible
            Uba1
            (Pfleger et al., 2007)
            hid
            suppressible
            Uba1
            (Christiansen et al., 2013, Lee et al., 2008, Pfleger et al., 2007)
            hpo
            enhanceable
            Uba1
            (Pfleger et al., 2007)
            External Data
            Linkouts
            BioGRID - A database of protein and genetic interactions.
            DroID - A comprehensive database of gene and protein interactions.
            MIST (genetic) - An integrated Molecular Interaction Database
            MIST (protein-protein) - An integrated Molecular Interaction Database
            Pathways
            Signaling Pathways (FlyBase)
            Metabolic Pathways
            FlyBase
            External Links
            FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel
            External Data
            Linkouts
            KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
            Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
            Class of Gene
            Genomic Location and Detailed Mapping Data
            Chromosome (arm)
            2R
            Recombination map
            2-61
            Cytogenetic map
            46A1-46A1
            Sequence location
            FlyBase Computed Cytological Location
            Cytogenetic map
            Evidence for location
            46A1-46A1
            Limits computationally determined from genome sequence between P{PZ}Mmp202353&P{PZ}Uba105642 and P{lacW}l(2)k09221k09221&P{PZ}dap04454
            Experimentally Determined Cytological Location
            Cytogenetic map
            Notes
            References
            46A-46A
            (Zur Lage, 1997.12.15)
            46A1-46A2
            (determined by in situ hybridisation)
            (Spradling et al., 1999)
            46A1-46A2
            46A2--3 46A2--4 46A3--4
            (BDGP Project Members, 1994-1999)
            Experimentally Determined Recombination Data
            Location

            2-61

            (FlyBase, 2016)

            2-59.5

            (Comeron et al., 2012)
            Left of (cM)
            Right of (cM)
            Notes
            Stocks and Reagents
            Stocks (25)
            Genomic Clones (19)
            cDNA Clones (184)
             

            Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

            cDNA clones, fully sequenced
            BDGP DGC clones
            Other clones
            Drosophila Genomics Resource Center cDNA clones

            For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

            cDNA Clones, End Sequenced (ESTs)
            BDGP DGC clones
            Other clones
            RNAi and Array Information
            Linkouts
            DRSC - Results frm RNAi screens
            Antibody Information
            Laboratory Generated Antibodies
             
            Commercially Available Antibodies
             
            Cell Line Information
            Publicly Available Cell Lines
             
              Other Stable Cell Lines
               
                Other Comments

                Uba1 controls apoptosis autonomously and tissue growth non-autonomously.

                (Lee et al., 2008)

                Uba1 mutations that appear to be hypomorphic elicit a mild overrepresentation of mutant tissue in a mosaic eye, while Uba1 mutations that represent strong or null alleles result in a cell-lethal phenotype that also stimulates overgrowth of surrounding wild-type tissue.

                (Pfleger et al., 2007)

                Expression is enriched in embryonic gonads.

                (Shigenobu et al., 2006)

                dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

                (Kiger et al., 2003)

                Uba1 is required for axon pruning during metamorphosis.

                (Watts et al., 2003)

                Mutants isolated in a screen of the second chromosome identifying genes affecting disc morphology.

                (Roch et al., 1998)
                Relationship to Other Genes
                Source for database merge of

                Source for merge of: Uba1 l(2)03405

                (Spradling et al., 1999)

                Source for merge of: Uba1 l(2)05642

                (Misra, 2000.11.18)
                Additional comments
                Nomenclature History
                Source for database identify of

                Source for identity of: Uba1 CG1782

                (Evangelista, 1999.11)
                Nomenclature comments
                Etymology
                Synonyms and Secondary IDs (21)
                Reported As
                Symbol Synonym
                2/31
                (Roch et al., 1998)
                CG1782
                (National Institute of Genetics Fly Stocks, 2022-, Kolkhof et al., 2017, Çiçek et al., 2016, Christis and Munro, 2012, Ni et al., 2011.7.19, Perkins et al., 2009, Perkins et al., 2009.1.15, Puri et al., 2008, Nakahara et al., 2005, Norga et al., 2003, Kraut et al., 2001, Kraut et al., 2001)
                DmUba1
                (Shih et al., 2002)
                E1
                (Liu and Pfleger, 2013, Barry et al., 2011, Bergmann, 2010, Yan et al., 2009, Yan et al., 2008, Canning and Finnegan, 1999)
                FBtr0088499
                (Li et al., 2012)
                UBA-1
                (Kurshakova et al., 2019, Yadav and Srikrishna, 2019, Zur Lage, 1997.12.15)
                UBA1
                (Bergmann, 2010)
                Uba
                (Meltzer et al., 2019)
                Uba 1
                (Kraut et al., 2001, Kraut et al., 2001)
                Uba1
                (Kashio and Miura, 2025, Dellar et al., 2024, Liu et al., 2024, Barbaste et al., 2023, Furusawa et al., 2023, Parkhitko et al., 2021, Zhang and Jiang, 2021, Xu et al., 2020, Levis, 2018.8.30, Ugrankar et al., 2018, Transgenic RNAi Project members, 2017-, Vazquez-Pianzola et al., 2017, Ashton-Beaucage et al., 2016, Kockel et al., 2016, Yadav et al., 2016, Yaniv and Schuldiner, 2016, Dent et al., 2015, Kanoh et al., 2015, Ugrankar et al., 2015, Chen et al., 2014, Colin et al., 2014, Lee et al., 2014, Mukherjee et al., 2014, Beckett et al., 2013, Chang et al., 2013, Kwon et al., 2013, Liu and Pfleger, 2013, Smibert et al., 2013, Li et al., 2012, Du et al., 2011, Franco et al., 2011, Lee et al., 2011, Kim et al., 2010, Saj et al., 2010, Sandu et al., 2010, Wasbrough et al., 2010, Carbone et al., 2009, Tan et al., 2009, Xu et al., 2009, Yan et al., 2009, FlyBase Curators, 2008-, Hoopfer et al., 2008, Lee et al., 2008, Schuldiner et al., 2008, Blard et al., 2007, Latouche et al., 2007, Pfleger et al., 2007, Shigenobu et al., 2006, Watts et al., 2003, Huet et al., 2002)
                l(2)03405
                (Meister and Braun, 1995.10, BDGP Project Members, 1994-1999)
                l(2)05642
                (BDGP Project Members, 1994-1999)
                l(2)s3484
                uba-1
                (Wing et al., 2002)
                uba1
                (Jia et al., 2015, Wong et al., 2013, Wu et al., 2011, Mudiganti et al., 2010, Zhang et al., 2010, Lee et al., 2008, Simonsen et al., 2007, Staudt et al., 2005)
                Name Synonyms
                E1
                (Murphey and Godenschwege, 2002)
                E1 ligase
                (Martin et al., 2014)
                Ubiquitin Activating Enzyme
                (Liu and Pfleger, 2013, Yan et al., 2008)
                Ubiquitin activating enzyme 1
                (Blard et al., 2007, Tadros et al., 2007)
                ubiquitin activating enzyme
                (Barry et al., 2011)
                ubiquitin activating enzyme 1
                (Beller et al., 2008)
                ubiquitin-activating enzyme (E1)
                (Speese et al., 2003)
                Secondary FlyBase IDs
                • FBgn0010538
                • FBgn0010605
                • FBgn0010688
                Datasets (0)
                Study focus (0)
                Experimental Role
                Project
                Project Type
                Title
                Study result (0)
                Result
                Result Type
                Title
                External Crossreferences and Linkouts ( 67 )
                Sequence Crossreferences
                NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
                GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
                GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
                RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
                UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
                UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
                Other crossreferences
                AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
                BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
                DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
                EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
                FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
                FlyMine - An integrated database for Drosophila genomics
                InterPro - A database of protein families, domains and functional sites
                KEGG Genes - Molecular building blocks of life in the genomic space.
                MARRVEL_MODEL - MARRVEL (model organism gene)
                Linkouts
                BioGRID - A database of protein and genetic interactions.
                Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
                DroID - A comprehensive database of gene and protein interactions.
                DRSC - Results frm RNAi screens
                Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
                FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
                FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
                FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel
                Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
                Flygut - An atlas of the Drosophila adult midgut
                FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
                iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
                Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
                KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
                MIST (genetic) - An integrated Molecular Interaction Database
                MIST (protein-protein) - An integrated Molecular Interaction Database
                Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
                References (159)