FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Gene: Dmel\Uba2
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General Information
Symbol
Dmel\Uba2
Species
D. melanogaster
Name
Ubiquitin-like activating enzyme 2
Annotation Symbol
CG7528
Feature Type
protein_coding_gene
FlyBase ID
FBgn0029113
Gene Model Status
Current
Stock Availability
8 publicly available
Gene Summary
Ubiquitin-like activating enzyme 2 (Uba2) encodes one of two subunits (along with the product of Aos1) of the heterodimeric SUMO activating enzyme. It contains the catalytic cysteine residue that first becomes adenylated using ATP and that then forms a thioester linkage to the C-terminal end of SUMO. [Date last reviewed: 2019-09-26] (FlyBase Gene Snapshot)
All Summaries
Also Known As

SAE2, sip2, DmUba2

Key Links
Genomic Location
Cytogenetic map
66B7-66B7
Sequence location
Recombination map
3-22
RefSeq locus
NT_037436 REGION:8125327..8127818
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (12 terms)
Molecular Function (3 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
contributes_to SUMO activating enzyme activity
inferred from direct assay
(Soory and Ratnaparkhi, 2022)
enables ubiquitin activating enzyme binding
inferred from physical interaction with FLYBASE:Aos1; FB:FBgn0029512
(Shih et al., 2002)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
enables SUMO activating enzyme activity
inferred from electronic annotation with InterPro:IPR030661
(InterPro Project Members, 2004-)
enables ubiquitin-like modifier activating enzyme activity
inferred from electronic annotation with InterPro:IPR035985
(InterPro Project Members, 2004-)
Biological Process (6 terms)
Terms Based on Experimental Evidence (6 terms)
CV Term
Evidence
References
acts_upstream_of negative regulation melanotic encapsulation of foreign target
inferred from mutant phenotype
(Paddibhatla et al., 2010)
involved_in negative regulation of receptor signaling pathway via JAK-STAT
inferred from mutant phenotype
(Vidal et al., 2010)
involved_in positive regulation of antimicrobial peptide production
inferred from mutant phenotype
(Kanoh et al., 2015)
involved_in positive regulation of Toll signaling pathway
inferred from mutant phenotype
(Kanoh et al., 2015)
involved_in protein sumoylation
inferred from mutant phenotype
(Kanakousaki and Gibson, 2012)
inferred from direct assay
(Andreev et al., 2022)
involved_in receptor-mediated endocytosis
inferred from mutant phenotype
(Vidal et al., 2010)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
involved_in protein sumoylation
inferred from electronic annotation with InterPro:IPR030661
(InterPro Project Members, 2004-)
inferred from biological aspect of ancestor with PANTHER:PTN000102239
(GO Reference Genome Project, 2011-)
Cellular Component (3 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
located_in cytoplasm
inferred from direct assay
(Shih et al., 2002)
located_in nucleus
inferred from direct assay
(Smith et al., 2004, Shih et al., 2002)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
is_active_in cytoplasm
inferred from biological aspect of ancestor with PANTHER:PTN000102040
(GO Reference Genome Project, 2011-)
part_of SUMO activating enzyme complex
inferred from biological aspect of ancestor with PANTHER:PTN000102239
(GO Reference Genome Project, 2011-)
Gene Group (FlyBase)
Pathway (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Snapshot
Ubiquitin-like activating enzyme 2 (Uba2) encodes one of two subunits (along with the product of Aos1) of the heterodimeric SUMO activating enzyme. It contains the catalytic cysteine residue that first becomes adenylated using ATP and that then forms a thioester linkage to the C-terminal end of SUMO. [Date last reviewed: 2019-09-26]
Pathway (FlyBase)
POSITIVE REGULATORS OF TOLL-NF-kappaB SIGNALING PATHWAY -
In Drosophila, the canonical Toll signaling pathway is initiated by the binding of a spatzle ligand to Toll (Tl) or a Toll-like receptor leading to the nuclear localization of the NF-κB (dl or Dif) transcription factor. Positive regulators of Toll-NF-κB Signaling lead to the increased nuclear accumulation of the NF-κB proteins and regulated expression of target genes. (Adapted from FBrf0091014 and FBrf0223077).
NEGATIVE REGULATORS OF JAK-STAT SIGNALING PATHWAY -
Negative regulators of JAK-STAT signaling down-regulate the pathway, attenuating transcriptional control by Stat92E.
Gene Group (FlyBase)
SUMO ACTIVATING ENZYMES -
SUMO activating enzymes are 'canonical' E1 enzymes that act as heterodimers to activate the ubiquitin-like protein SUMO for subsequent steps along the SUMO conjugation cascade. They perform three distinct chemical reactions: first, they bind ATP, magnesium and SUMO to catalyze formation of a high-energy acyl adenylate intermediate with subsequent release of pyrophosphate; second, an E1 catalytic cysteine attacks the SUMO-adenylate to catalyze formation of a high-energy thioester bond between the E1 and SUMO and release of the AMP product; finally, the E1-SUMO catalyzes transthioesterification to an E2 to form a high energy thioester-linked E2-SUMO product. (Adapted from PMID:28234446.)
Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\Uba2 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q7KUA4)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Gene model reviewed during 5.45

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Uba2-RA
FBtr0076734
NM_080017
2329
700
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
Uba2-PA
FBpp0076457
77.6
700
4.63
Q7KUA4
NP_524756
AAF50484
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)

700 (aa)

(Bhaskar et al., 2000)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Uba2 using the Feature Mapper tool.

External Data
Crossreferences
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

0.01

Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 3
organism

Comment: maternally deposited

(Fisher et al., 2012)
embryonic stage 4 -- 6
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 5 -- 10
organism | ubiquitous
(Hashiyama et al., 2009)
embryonic stage 7 -- 8
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 9 -- 10
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 11 -- 12
anterior midgut primordium
(Fisher et al., 2012)
central brain primordium
(Fisher et al., 2012)
head mesoderm
(Fisher et al., 2012)
organism | ubiquitous
(Fisher et al., 2012)
posterior midgut primordium
(Fisher et al., 2012)
trunk mesoderm
(Fisher et al., 2012)
ventral nerve cord primordium
(Fisher et al., 2012)
embryonic stage 13 -- 16
embryonic brain
(Fisher et al., 2012)
embryonic/larval lymph gland
(Fisher et al., 2012)
larval nervous system
(Fisher et al., 2012)
gonad
(Fisher et al., 2012)
larval ventral nerve cord
(Fisher et al., 2012)
embryonic stage 14 -- 17
gonad
(Hashiyama et al., 2009)
primordial germ cell
(Hashiyama et al., 2009)
embryonic stage 15
larval central nervous system
(Hashiyama et al., 2009)
oogenesis
germarium
(Hashiyama et al., 2009)
oogenesis stage S1 -- S10
nurse cell
(Hashiyama et al., 2009)
oocyte
(Hashiyama et al., 2009)
spermatogenesis
spermatogonium
(Hashiyama et al., 2009)
spermatocyte
(Hashiyama et al., 2009)
spermatid
(Hashiyama et al., 2009)
Additional Descriptive Data

Maternal Uba2 transcripts are distributed throughout the embryo until stage 10 and then decrease gradually. From stage 14 until the end of embryogenesis, transcripts are observed in the embryonic gonads in the pole cells. Uba2 is also strongly expressed in the CNS. Within ovaries, transcripts are weakly detected in the germarium, are strongly expressed in stage S1-10 nurse cells and are weakly detected in the oocyte up until stage S10. Uba2 transcripts are weakly expressed in spermatogonial cells at the tip region and remain detectable in the spermatocytes and spermatids.

(Hashiyama et al., 2009)
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic cycle 10 -- embryonic cycle 14
organism
• nucleus

Comment: interphase

(Shih et al., 2002)

Comment: mitosis

(Shih et al., 2002)
embryonic stage 6 -- 15
organism

Comment: mitosis

(Shih et al., 2002)
organism
• nucleus

Comment: interphase

(Shih et al., 2002)
adult stage
germarium
• nucleus
(Shih et al., 2002)
oocyte
• nucleus
(Shih et al., 2002)
Additional Descriptive Data

During embryonic cycles 10-14 protein was detected distributes around the cortex during mitotic divisions but concentrated in the nuclei during interphase. In later embryos postcellularization protein was detected in the cytoplasm of mitotic cells and primarily in the nuclei of interphase cells. No substantial colocalization with Septins was observed. During oogenesis Uba2 protein was detected in the nuclei of oocytes and of the follicle cells in the germarium.

(Shih et al., 2002)
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in cytoplasm
inferred from direct assay
(Shih et al., 2002)
located_in nucleus
inferred from direct assay
(Smith et al., 2004, Shih et al., 2002)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Uba2 in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 4-6
  • Stages(s) 7-8
  • Stages(s) 9-10
  • Stages(s) 11-12
  • Stages(s) 13-16
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 5 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 11 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Uba2
Transgenic constructs containing regulatory region of Uba2
Aberrations (Deficiencies and Duplications) ( 1 )
Inferred from experimentation ( 1 )
Gene disrupted in
Df(3L)Exel6112
(Ryder, 2004.4.26)
Inferred from location ( 8 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
cell lethal | somatic clone | third instar larval stage
lethal (with Df(3L)Exel6112)
lethal - all die before end of P-stage | recessive
Sterility
Allele
female fertile, with Scer\GAL4nanos.PG
Other Phenotypes
Allele
abnormal immune response, with Scer\GAL4Cg.PA
abnormal immune response, with Scer\GAL4Hml.PG
abnormal immune response, with Scer\GAL4Lsp2.PH
decreased cell number | somatic clone | third instar larval stage
increased cell death | somatic clone | third instar larval stage
increased cell death | temperature conditional, with Scer\GAL4GMR.PF
some die during P-stage | recessive
visible, with Scer\GAL4ey.PU
visible, with Scer\GAL4GMR.PF
Phenotype manifest in
Allele
adult head, with Scer\GAL4ey.PU
adult optic lobe
embryonic/larval fat body, with Scer\GAL4Cg.PA
embryonic/larval fat body, with Scer\GAL4Hml.PG
embryonic/larval hemocoel, with Scer\GAL4Cg.PA
embryonic/larval hemocoel, with Scer\GAL4Hml.PG
embryonic/larval hemocoel, with Scer\GAL4Lsp2.PH
eye, with Scer\GAL4ey.PU
eye, with Scer\GAL4GMR.PF
eye | temperature conditional, with Scer\GAL4GMR.PF
eye disc, with Scer\GAL4ey.PU
imaginal disc
ommatidium, with Scer\GAL4GMR.PF
ommatidium | temperature conditional, with Scer\GAL4GMR.PF
pigment cell, with Scer\GAL4GMR.PF
wing disc | somatic clone
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (10)
Hsap\UBA2
14 of 14
Yes
Yes
Hsap\UBA3
3 of 14
No
No
Hsap\MOCS3
2 of 14
No
No
Hsap\NAE1
2 of 14
No
No
Hsap\SAE1
2 of 14
No
No
Hsap\UBA1
2 of 14
No
No
Hsap\UBA5
2 of 14
No
No
18  
Hsap\UBA6
2 of 14
No
No
Hsap\UBA7
2 of 14
No
No
1  
Hsap\ATG7
1 of 14
No
No
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (10)
Rnor\Uba2
14 of 14
Yes
Yes
Rnor\Uba3
3 of 14
No
No
Rnor\Mocs3
2 of 14
No
No
Rnor\Sae1
2 of 14
No
No
Rnor\Uba1
2 of 14
No
No
Rnor\Uba5
2 of 14
No
No
Rnor\Uba6
2 of 14
No
No
Rnor\Atg7
1 of 14
No
No
Rnor\Uba1y
1 of 14
No
No
Rnor\Uba7
1 of 14
No
No
Mus musculus (laboratory mouse) (11)
Mmus\Uba2
14 of 14
Yes
Yes
Mmus\Mocs3
2 of 14
No
No
Mmus\Sae1
2 of 14
No
No
Mmus\Uba1
2 of 14
No
No
Mmus\Uba3
2 of 14
No
No
Mmus\Uba5
2 of 14
No
No
Mmus\Uba6
2 of 14
No
No
Mmus\Uba7
2 of 14
No
No
Mmus\Atg7
1 of 14
No
No
Mmus\Nae1
1 of 14
No
No
Mmus\Uba1y
1 of 14
No
No
Xenopus tropicalis (Western clawed frog) (7)
Xtro\uba2
11 of 13
Yes
Yes
Xtro\mocs3
1 of 13
No
No
Xtro\nae1
1 of 13
No
No
Xtro\qtrt1
1 of 13
No
No
Xtro\sae1
1 of 13
No
No
Xtro\uba3
1 of 13
No
No
Xtro\uba5
1 of 13
No
No
Danio rerio (Zebrafish) (9)
Drer\uba2
13 of 14
Yes
Yes
Drer\uba3
3 of 14
No
No
Drer\mocs3
2 of 14
No
No
Drer\sae1
2 of 14
No
No
Drer\uba1
2 of 14
No
No
Drer\nae1
1 of 14
No
No
Drer\uba5
1 of 14
No
No
Drer\uba6
1 of 14
No
No
Drer\uba7
1 of 14
No
No
Caenorhabditis elegans (Nematode, roundworm) (8)
Cele\uba-2
13 of 14
Yes
Yes
Cele\rfl-1
3 of 14
No
No
Cele\aos-1
2 of 14
No
No
Cele\moc-3
2 of 14
No
No
Cele\uba-5
2 of 14
No
No
Cele\ula-1
2 of 14
No
No
Cele\atg-7
1 of 14
No
No
Cele\uba-1
1 of 14
No
No
Anopheles gambiae (African malaria mosquito) (8)
Agam\AgaP_AGAP008637
12 of 12
Yes
Yes
Agam\AgaP_AGAP005102
3 of 12
No
No
Agam\AgaP_AGAP011872
3 of 12
No
No
Agam\AgaP_AGAP005544
2 of 12
No
No
Agam\AgaP_AGAP009012
2 of 12
No
No
Agam\MOCS3_ANOGA
2 of 12
No
No
Agam\AgaP_AGAP004459
1 of 12
No
No
Agam\AgaP_AGAP010303
1 of 12
No
No
Arabidopsis thaliana (thale-cress) (15)
Atha\SAE2
13 of 13
Yes
Yes
Atha\ECR1
3 of 13
No
No
Atha\AT1G05350
2 of 13
No
No
Atha\AXL
2 of 13
No
No
Atha\AXR1
2 of 13
No
No
Atha\CNX5
2 of 13
No
No
Atha\SAE1A
2 of 13
No
No
Atha\SAE1B
2 of 13
No
No
Atha\SAE1B
2 of 13
No
No
Atha\UBA1
2 of 13
No
No
Atha\UBA 2
2 of 13
No
No
Atha\APG7
1 of 13
No
No
Atha\AT3G25880
1 of 13
No
No
Atha\AT5G19370
1 of 13
No
No
Atha\AT5G37530
1 of 13
No
Yes
Saccharomyces cerevisiae (Brewer's yeast) (9)
Scer\UBA2
13 of 13
Yes
Yes
Scer\AOS1
2 of 13
No
No
Scer\UBA1
2 of 13
No
No
Scer\UBA3
2 of 13
No
No
Scer\UBA4
2 of 13
No
No
Scer\ULA1
2 of 13
No
No
Scer\ATG7
1 of 13
No
No
Scer\TCD1
1 of 13
No
Yes
Scer\TCD2
1 of 13
No
No
Schizosaccharomyces pombe (Fission yeast) (8)
Spom\fub2
12 of 12
Yes
Yes
Spom\ptr3
2 of 12
No
No
Spom\SPAC2G11.10c
2 of 12
No
No
Spom\uba3
2 of 12
No
No
Spom\atg7
1 of 12
No
No
Spom\rad31
1 of 12
No
No
Spom\SPAC1A6.10
1 of 12
No
Yes
Spom\uba5
1 of 12
No
No
Escherichia coli (enterobacterium) (3)
Ecol\moeB
3 of 11
Yes
No
Ecol\thiF
3 of 11
Yes
No
Ecol\tcdA
1 of 11
No
Yes
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:Uba2. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (8)
Uba3
5 of 13
Aos1
4 of 13
APP-BP1
4 of 13
Mocs3
4 of 13
Uba1
4 of 13
Uba5
4 of 13
Atg7
3 of 13
mRpL36
1 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Allele
Disease
Evidence
References
Uba2HM05055
model of  hematologic cancer
CEA
(Paddibhatla et al., 2010)
Potential Models Based on Orthology ( 0 )
Human Ortholog
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Allele
Disease
Interaction
References
Uba2C175S.UAS
exacerbates  Kennedy's disease
modeled by Hsap\ARQ112.UAS.Tag:HA
(Chan et al., 2002)
Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Homo sapiens (Human)
Gene name
Score
OMIM
OMIM Phenotype
DO term
Complementation?
Transgene?
UBA2; ubiquitin like modifier activating enzyme 2
14 of 14
613295
    UBA3; ubiquitin like modifier activating enzyme 3
    3 of 14
    603172
        Functional Complementation Data
        Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
        Interactions
        Summary of Physical Interactions
        Summary of Genetic Interactions
        Interaction Browsers
        View in FlyBase Interactions Browser   View in MIST tool (external link)
        Please look at the allele data for full details of the genetic interactions
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        Dcp-1
        suppressible
        Uba2
        (Kim et al., 2010)
        Dcp-1
        enhanceable
        Uba2
        (Kim et al., 2010)
        Hsp60D
        enhanceable
        Uba2
        (Arya et al., 2010)
        External Data
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        DroID - A comprehensive database of gene and protein interactions.
        MIST (genetic) - An integrated Molecular Interaction Database
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Pathways
        Signaling Pathways (FlyBase)
        Metabolic Pathways
        FlyBase
        External Links
        External Data
        Linkouts
        KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
        Class of Gene
        Genomic Location and Detailed Mapping Data
        Chromosome (arm)
        3L
        Recombination map
        3-22
        Cytogenetic map
        66B7-66B7
        Sequence location
        FlyBase Computed Cytological Location
        Cytogenetic map
        Evidence for location
        66B7-66B7
        Limits computationally determined from genome sequence between P{PZ}l(3)0721708223&P{EP}Rac2EP3118 and P{lacW}Nmtj1C7
        Experimentally Determined Cytological Location
        Cytogenetic map
        Notes
        References
        66B6-66B10
        (Shih et al., 2002)
        65B5-65C3
        (determined by in situ hybridisation)
        (Long and Griffith, 2000)
        66B6-66B11
        (Shih, 1999.10.8)
        66B5-66B11
        (Shih, 1998.3.17)
        Cytological location based on P1 blot screening and PCR checking.
        (Shih, 1998.3.17)
        Mapped using a high-density filter of P1 clones.
        (Shih et al., 2002)
        Experimentally Determined Recombination Data
        Location

        3-22

        (FlyBase, 2016)

        3-19.0

        (Comeron et al., 2012)
        Left of (cM)
        Right of (cM)
        Notes
        Stocks and Reagents
        Stocks (8)
        Genomic Clones (17)
         

        Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

        cDNA Clones (155)
         

        Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

        cDNA clones, fully sequenced
        BDGP DGC clones
        Other clones
          Drosophila Genomics Resource Center cDNA clones

          For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

          cDNA Clones, End Sequenced (ESTs)
          BDGP DGC clones
          Other clones
          RNAi and Array Information
          Linkouts
          DRSC - Results frm RNAi screens
          Antibody Information
          Laboratory Generated Antibodies
           

          polyclonal

          (Shih et al., 2002, Donaghue et al., 2001, Long and Griffith, 2000)
          Commercially Available Antibodies
           
          Cell Line Information
          Publicly Available Cell Lines
           
            Other Stable Cell Lines
             
              Other Comments

              Expression is enriched in embryonic gonads.

              (Shigenobu et al., 2006)

              Identification: Yeast two-hybrid screen for proteins that interact with the pnut, Sep1 or Sep2 proteins. Uba2 interacts with Sep1 and Sep2 in the two-hybrid system and does not interact with pnut.

              (Shih et al., 1998)
              Relationship to Other Genes
              Source for database merge of

              Source for merge of: Uba2 SAE2 CG7528

              (Bayraktaroglu, 2000.5.25)
              Additional comments

              FlyBase curator comment: the symbol "sip2" has been used to refer to two genes; both the gene referred to in FlyBase as Uba2 (CG7528, FBgn0029113) - in FBrf0108803, FBrf0102083 and FBrf0101408 and also the gene referred to in FlyBase as sip2 (CG9188, FBgn0031878) - in FBrf0144953.

              (Shih, 2003.6.25)

              FlyBase curator comment: the gene referred to as "sip2" in FBrf0108803 corresponds to the gene referred to in FlyBase as Uba2 (CG7528, FBgn0029113) and does NOT correspond to the gene referred to in FlyBase as sip2 (CG9188, FBgn0031878).

              (Hsin-Pei et al., 1998)

              FlyBase curator comment: the gene referred to as "sip2" in FBrf0101408 corresponds to the gene referred to in FlyBase as Uba2 (CG7528, FBgn0029113) and does NOT correspond to the gene referred to in FlyBase as sip2 (CG9188, FBgn0031878).

              (Shih et al., 1998)

              FlyBase curator comment: the gene referred to as "sip2" in FBrf0102083 corresponds to the gene referred to in FlyBase as Uba2 (CG7528, FBgn0029113) and does NOT correspond to the gene referred to in FlyBase as sip2 (CG9188, FBgn0031878).

              (Shih, 1998.3.17)
              Nomenclature History
              Source for database identify of
              Nomenclature comments
              Etymology
              Synonyms and Secondary IDs (19)
              Reported As
              Symbol Synonym
              CG7528
              (Meadows, 2017.1.11, Neuert et al., 2017, Schwartz et al., 2016, Chang et al., 2013, Christis and Munro, 2012, Ni et al., 2011.7.19, Vidal et al., 2010, Keleman et al., 2009.8.5, Perkins et al., 2009, Talamillo et al., 2008, Dorer et al., 2006, Suzuki et al., 2001, Bayraktaroglu, 2000.5.25)
              DmSAE2
              (Bhaskar et al., 2000)
              DmSae2
              (Smith et al., 2001)
              DmUba2
              (Shih, 2003.6.25, Shih et al., 2002, Long and Griffith, 2000)
              Dmuba2
              (Shih et al., 2000)
              L36
              (Suzuki et al., 2001)
              SAE2
              (Nie et al., 2009, Smith et al., 2004, Bhaskar et al., 2002, Bayraktaroglu, 2000.5.25, Bhaskar, 1999.12.27)
              Sae2
              (Smith et al., 2002)
              UBA2
              (Pardi et al., 2011, Dorer et al., 2006)
              Uba2
              (Ko et al., 2024, Yheskel et al., 2023, Andreev et al., 2022, Cao et al., 2022, Okuda et al., 2022, Pavlidaki et al., 2022, Soory and Ratnaparkhi, 2022, Ninova et al., 2020, Myat et al., 2019, Neuert et al., 2017, Kanoh et al., 2015, Yan et al., 2014, Chang et al., 2013, Czech et al., 2013, Japanese National Institute of Genetics, 2012.5.21, Takács et al., 2012, Du et al., 2011, Arya et al., 2010, Kim et al., 2010, Paddibhatla et al., 2010, Vidal et al., 2010, Zhang et al., 2010, Hashiyama et al., 2009, Jacques et al., 2009, Talamillo et al., 2008, Altenhein et al., 2006, Shigenobu et al., 2006, Chan et al., 2002)
              Ubc2
              (Toba and White, 2003)
              dUba2
              (Donaghue et al., 2001)
              sip2
              (Shih, 2003.6.25, Hsin-Pei et al., 1998, Shih, 1998.3.17, Shih et al., 1998)
              uba2
              (Gao et al., 2024, Muerdter et al., 2013, Kanakousaki and Gibson, 2012, Pardi et al., 2011, Kim et al., 2010, Zhang et al., 2010)
              Name Synonyms
              Smt3 activating enzyme 2
              (Bhaskar, 1999.12.27)
              Smt3-activating enzyme 2
              (Kiss et al., 2005)
              Ubiquitin activating enzyme 2
              Ubiquitin-like activating enzyme 2
              ubiquitin-activating enzyme (E1)
              (Speese et al., 2003)
              Secondary FlyBase IDs
              • FBgn0024190
              • FBgn0035840
              • FBgn0040287
              Datasets (0)
              Study focus (0)
              Experimental Role
              Project
              Project Type
              Title
              Study result (0)
              Result
              Result Type
              Title
              External Crossreferences and Linkouts ( 47 )
              Sequence Crossreferences
              NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
              GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
              GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
              RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
              UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
              UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
              Other crossreferences
              AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
              BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
              DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
              EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
              FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
              FlyMine - An integrated database for Drosophila genomics
              InterPro - A database of protein families, domains and functional sites
              KEGG Genes - Molecular building blocks of life in the genomic space.
              MARRVEL_MODEL - MARRVEL (model organism gene)
              Linkouts
              BioGRID - A database of protein and genetic interactions.
              Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
              DroID - A comprehensive database of gene and protein interactions.
              DRSC - Results frm RNAi screens
              FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
              FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
              Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
              Flygut - An atlas of the Drosophila adult midgut
              iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
              KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
              MIST (genetic) - An integrated Molecular Interaction Database
              MIST (protein-protein) - An integrated Molecular Interaction Database
              References (91)