FB2021_02, released April 13, 2021

Physical Interaction report

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General Information
Interaction Type
physical association
Interacting Genes
p53, Uba2
FlyBase ID
FBig0000096143
Interaction Network
Interactions Browser links
p53 network
Uba2 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0216737-1.Y2H
Reference
Pardi et al., 2011
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
p53 

Dmp53

bait
Uba2 

UBA2

prey
Uba2 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Uba2 
C-terminal region
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryonic cDNA library.

(Pardi et al., 2011)

Two-hybrid system: yeast LexA/VP16.

(Pardi et al., 2011)
FBrf0216737-4.PD.A
Reference
Pardi et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
p53 

Dmp53

bait

GST pull down

Uba2 

UBA2

prey

[3]H label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Cloned reagents; interaction in solution.

(Pardi et al., 2011)
FBrf0216737-8.Y2H
Reference
Pardi et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
p53 

Dmp53

bait
p53 
p53 
Uba2 

UBA2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
p53 
C- terminal region
sufficient binding region
aa 277-385
p53 
K302R
unspecified role
aa 302
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Prey was cloned reagent (previously isolated).

(Pardi et al., 2011)

Two-hybrid system: yeast LexA/VP16.

(Pardi et al., 2011)

Weak interaction observed with smaller p53 C-terminal fragment, basic region C2 (352-385).

(Pardi et al., 2011)

K302R modification of aa 302 (within a canonical SUMO acceptor site) significantly weakens this interaction.

(Pardi et al., 2011)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)