Sep2
fused to LexA DNA-binding domain
DmUba2
fused to B42 activation domain
coordinates relative to Uba2-PA
Two-hybrid system: yeast LexA-BD/B42-AD
1 Uba2 clone obtained in the Sep2 screen.
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).